The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Discovery of the Novel Candidate Phylum “ Poribacteria ” in Marine Sponges
Open accessAbstract
Marine sponges ( Porifera ) harbor large amounts of commensal microbial communities within the sponge mesohyl. We employed 16S rRNA gene library construction using specific PCR primers to provide insights into the phylogenetic identity of an abundant sponge-associated bacterium that is morphologically characterized by the presence of a membrane-bound nucleoid. In this study, we report the presence of a previously unrecognized evolutionary lineage branching deeply in the domain Bacteria that is moderately related to the Planctomycetes , Verrucomicrobia , and Chlamydia lines of decent. Because members of this lineage showed <75% 16S rRNA gene sequence similarity to known bacterial phyla, we suggest the status of a new candidate phylum, named “ Poribacteria ”, to acknowledge the affiliation of the new bacterium with sponges. The affiliation of the morphologically conspicuous sponge bacterium with the novel phylogenetic lineage was confirmed by fluorescence in situ hybridization with newly designed probes targeting different sites of the poribacterial 16S rRNA. Consistent with electron microscopic observations of cell compartmentalization, the fluorescence signals appeared in a ring-shaped manner. PCR screening with “ Poribacteria ”-specific primers gave positive results for several other sponge species, while samples taken from the environment (seawater, sediments, and a filter-feeding tunicate) were PCR negative. In addition to a report for Planctomycetes , this is the second report of cell compartmentalization, a feature that was considered exclusive to the eukaryotic domain, in prokaryotes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Phylogenetic identification and in situ detection of individual microbial cells without cultivation
Amann, R., Ludwig, W., & SCHLEIFER, K. H. (1995) · Microbiological Reviews · doi:10.1128/mmbr.59.1.143-169.1995 · Cited by 9,093
Woese, C. R. (1987) · Microbiological Reviews · doi:10.1128/mr.51.2.221-271.1987 · Cited by 5,042
Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity
Hugenholtz, P., Goebel, B. M., & Pace, N. R. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.18.4765-4774.1998 · Cited by 2,428
Daims, H., Brühl, A., Amann, R., Schleifer, K.-H., & Wagner, M. (1999) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(99)80053-8 · Cited by 2,274
Self-citationCole, J. R. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg039 · Cited by 1,429
Systema Porifera. A Guide to the Classification of Sponges
Hooper, J. N. A., & Van Soest, R. W. M. (2002) · Systema Porifera · doi:10.1007/978-1-4615-0747-5_1 · Cited by 1,188
Novel Division Level Bacterial Diversity in a Yellowstone Hot Spring
Hugenholtz, P., Pitulle, C., Hershberger, K. L., & Pace, N. R. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.2.366-376.1998 · Cited by 895
Dojka, M. A., Hugenholtz, P., Haack, S. K., & Pace, N. R. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.10.3869-3877.1998 · Cited by 762
Molecular Evidence for a Uniform Microbial Community in Sponges from Different Oceans
Hentschel, U., Hopke, J., Horn, M., Friedrich, A. B., Wagner, M., Hacker, J., & Moore, B. S. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.9.4431-4440.2002 · Cited by 703
Self-citationSchmid, M., Twachtmann, U., Klein, M., Strous, M., Juretschko, S., Jetten, M., Metzger, J. W., Schleifer, K.-H., & Wagner, M. (2000) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(00)80050-8 · Cited by 687
Self-citation
Cited by
Bhalerao, S., & Kadam, P. (2010) · International Journal of Ayurveda Research · doi:10.4103/0974-7788.59946 · Cited by 798
Genomic insights into the marine sponge microbiome
Hentschel, U., Piel, J., Degnan, S. M., & Taylor, M. W. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2839 · Cited by 658
Self-citationDo associated microbial abundances impact marine demosponge pumping rates and tissue densities?
Weisz, J. B., Lindquist, N., & Martens, C. S. (2008) · Oecologia · doi:10.1007/s00442-007-0910-0 · Cited by 309
Siegl, A., Kamke, J., Hochmuth, T., Piel, J., Richter, M., Liang, C., Dandekar, T., & Hentschel, U. (2011) · The ISME Journal · doi:10.1038/ismej.2010.95 · Cited by 276
Self-citationGlansdorff, N., Xu, Y., & Labedan, B. (2008) · Biology Direct · doi:10.1186/1745-6150-3-29 · Cited by 264
Metadata
- Authors
- Fieseler, L., Horn, M., Wagner, M., & Hentschel, U.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-06
- Volume
- 70
- Issue
- 6
- Pages
- 3724-3732
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1985898172
- Cited by
- 301
- References
- 45
- FWCI
- 12.22
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Discovery of the Novel Candidate Phylum “ Poribacteria ” in Marine Sponges. https://app.cochat.ai/papers/10.1128/aem.70.6.3724-3732.2004
- The paper
- Fieseler, L., Horn, M., Wagner, M., & Hentschel, U. (2004). Discovery of the Novel Candidate Phylum “ Poribacteria ” in Marine Sponges. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.70.6.3724-3732.2004
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
