Multiplex PCR: Optimization and Application in Diagnostic Virology

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The elimination of primer-dimer accumulation in PCR

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Multiplex detection of four pathogenic retroviruses using molecular beacons

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Multiplex PCR for Typing and Subtyping Influenza and Respiratory Syncytial Viruses

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Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers

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Betaine can eliminate the base pair composition dependence of DNA melting

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Surveys of Gene Families Using Polymerase Chain Reaction: PCR Selection and PCR Drift

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Optimization and troubleshooting in PCR.Preprint

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Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single-Tube Multiplex Reverse Transcription-PCR: Feasibility Study

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Specificity, efficiency, and fidelity of PCR.Preprint

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MULTIPLEX PCR FOR THE DIAGNOSIS OF DUCHENNE MUSCULAR DYSTROPHY

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Rhinoviruses replicate effectively at lower airway temperatures

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Rapid Simultaneous Diagnosis of Infections with Respiratory Syncytial Viruses A and B, Influenza Viruses A and B, and Human Parainfluenza Virus Types 1, 2, and 3 by Multiplex Quantitative Reverse Transcription–Polymerase Chain Reaction–Enzyme Hybridization Assay (Hexaplex)

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Laboratory Diagnosis of Common Viral Infections of the Central Nervous System by Using a Single Multiplex PCR Screening Assay

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PCR bias in amplification of androgen receptor alleles, a trinucleotide repeat marker used in clonality studies

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The Effect of Temperature and Oligonucleotide Primer Length on the Specificity and Efficiency of Amplification by the Polymerase Chain Reaction

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Use of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions

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Multiplex reverse transcription-PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996

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Advances in nucleic acid-based detection methods

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Single-Step Multiplex PCR Assay for Characterization of New World Leishmania Complexes

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Simplified hot start PCR

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Reactivation of herpes simplex virus type 1 in patients with Bell's palsy

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Comparison of Clinical Diagnosis and Standard Laboratory and Molecular Methods for the Diagnosis of Genital Ulcer Disease in Lesotho: Association with Human Immunodeficiency Virus Infection

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Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory

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Amplification of Nucleic Acids by Polymerase Chain Reaction (PCR) and Other Methods and their Applications

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The clinical diagnosis of genital ulcer disease in men.

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Detection and identification of vaccine-related polioviruses by the polymerase chain reaction

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Direct Detection of Respiratory Syncytial Virus, Parainfluenza Virus, and Adenovirus in Clinical Respiratory Specimens by a Multiplex Reverse Transcription-PCR Assay

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Simultaneous Detection and Identification of Human Parainfluenza Viruses 1, 2, and 3 from Clinical Samples by Multiplex PCR

Echevarría, J. E., Erdman, D. D., Swierkosz, E. M., et al. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.5.1388-1391.1998 · Cited by 136 · see citing works

Simplified polymerase chain reaction detection of new world Leishmania in clinical specimens of cutaneous leishmaniasis.

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Rapid and sensitive polymerase chain reaction based detection and typing of foot-and-mouth disease virus in clinical samples and cell culture isolates, combined with a simultaneous differentiation with other genomically and/or symptomatically related viruses

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A commentary on the practical applications of competitive PCR.Preprint

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A simplified procedure for developing multiplex PCRs.Preprint

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Genetic Reassortment among Viruses Causing Hantavirus Pulmonary Syndrome

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A practical approach to genetic screening for influenza virus variants

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Efficient 12-mutation testing in the CFTR gene: a general model for complex mutation analysis

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Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses

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Detection of enteroviral RNA and specific DNA of herpesviruses by multiplex genome amplification

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Detection and typing of human herpesviruses by multiplex polymerase chain reaction

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Molecular Methods for the Diagnosis of Genital Ulcer Disease in a Sexually Transmitted Disease Clinic Population in Northern Thailand: Predominance of Herpes Simplex Virus Infection

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Erythrocyte 3-O-methyl-D-glucose uptake assay for diagnosis of glucose-transporter-protein syndrome

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Sensitive and Rapid Detection of Viable Giardia Cysts and Cryptosporidium parvum Oocysts in Large-Volume Water Samples with Wound Fiberglass Cartridge Filters and Reverse Transcription-PCR

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Detection of hog cholera virus and differentiation from other pestiviruses by polymerase chain reaction

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Differentiation ofCampylobacter jejuniandCampylobacter coliby polymerase chain reaction

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Viral serologies in patients with chronic fatigue and chronic fatigue syndrome

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Multiplex PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Genitourinary specimens

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Detection and typing of lymphotropic herpesviruses by multiplex polymerase chain reaction

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Advantages of a New Taq DNA Polymerase in Multiplex PCR and Time-Release PCR

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Herpes Simplex Virus Infections of the Central Nervous System in Human Immunodeficiency Virus–Infected Patients: Clinical Management by Polymerase Chain Reaction Assay of Cerebrospinal Fluid

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Genotyping by multiplex polymerase chain reaction for detection of endemic hepatitis B virus transmission

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Amplification of the Six Major Human Herpesviruses from Cerebrospinal Fluid by a Single PCR

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Fast multiplex polymerase chain reaction on boiled clinical samples for rapid viral diagnosis

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Assessment of diagnostic quantitative fluorescent multiplex polymerase chain reaction assays performed on single cells

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Diagnosis of equine gammaherpesvirus 2 and 5 infections by polymerase chain reaction

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Multiplex PCR for diagnosis of AIDS-related central nervous system lymphoma and toxoplasmosis

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Diagnosis of viral and chlamydial keratoconjunctivitis: which laboratory test?

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Nested and multiplex polymerase chain reactions for the identification of bluetongue virus infection in the biting midge, Culicoides variipennis

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Optimization of Multiplex PCRs

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Optimizing multiplex and LA-PCR with betaine

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Technical report: Part 2. Basic requirements for designing optimal PCR primers

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Rapid detection of Actinobacillus actinomycetemcomitans, Prevotella intermedia and Porphyromona gingivalis by multiplex PCR

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Multiplex polymerase chain reaction for adenovirus and herpes simplex virus in eye swabs

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Conducting polymers on microelectronic devices as tools for biological analyses

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Detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Ureaplasma urealyticum, and Mycoplasma genitalium in first-void urine specimens by multiplex polymerase chain reaction

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Detection of parvovirus B19 by dot-blot and polymerase chain reaction

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Combining Multiplex and Touchdown PCR to Screen Murine Microsatellite Polymorphisms

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The design of strain-specific polymerase chain reactions for discrimination of the racoon rabies virus strain fron indigenous rabies viruses of Ontario

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Identification of New Influenza B Virus Variants by Multiplex Reverse Transcription-PCR and the Heteroduplex Mobility Assay

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Detection of human herpes virus 6 in AIDS‐associated retinitis by means of in situ hybridization, polymerase chain reaction and immunohistochemistry

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Development of a multiplex PCR assay for the simultaneous detection and discrimination of HIV-1, HIV-2, HTLV-I and HTLV-II

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Quantitative Multiple Competitive PCR of HIV-1 DNA in a Single Reaction Tube

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Direct Detection of Sabin Poliovirus Vaccine Strains in Stool Specimens of First-Dose Vaccinees by a Sensitive Reverse Transcription-PCR Method

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Transcription of the human cytomegalovirus natural killer decoy gene, UL18, in vitro and in vivo.

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Multicenter International Work Flow Study of an Automated Polymerase Chain Reaction Instrument

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