The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing
Open accessAbstract
SUMMARY: Real-time PCR has revolutionized the way clinical microbiology laboratories diagnose many human microbial infections. This testing method combines PCR chemistry with fluorescent probe detection of amplified product in the same reaction vessel. In general, both PCR and amplified product detection are completed in an hour or less, which is considerably faster than conventional PCR detection methods. Real-time PCR assays provide sensitivity and specificity equivalent to that of conventional PCR combined with Southern blot analysis, and since amplification and detection steps are performed in the same closed vessel, the risk of releasing amplified nucleic acids into the environment is negligible. The combination of excellent sensitivity and specificity, low contamination risk, and speed has made real-time PCR technology an appealing alternative to culture- or immunoassay-based testing methods for diagnosing many infectious diseases. This review focuses on the application of real-time PCR in the clinical microbiology laboratory.
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Lanciotti, R. S., Kerst, A. J., Nasci, R. S., Godsey, M. S., Mitchell, C. J., Savage, H. M., Komar, N., Panella, N. A., Allen, B. C., Volpe, K. E., et al. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.11.4066-4071.2000 · Cited by 1,087
Perandin, F., Manca, N., Calderaro, A., Piccolo, G., Galati, L., Ricci, L., Medici, M. C., Arcangeletti, M. C., Snounou, G., Dettori, G., et al. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.3.1214-1219.2004 · Cited by 354
Ramers, C. (2000) · JAMA · doi:10.1001/jama.283.20.2680 · Cited by 291
National disease burden of respiratory viruses detected in children by polymerase chain reaction
HENRICKSON, K. J., HOOVER, S., KEHL, K. S., & HUA, W. (2004) · Pediatric Infectious Disease Journal · doi:10.1097/01.inf.0000108188.37237.48 · Cited by 250
Teramura, T., Tabata, Y., Yagi, T., Morimoto, A., Hibi, S., & Imashuku, S. (2002) · Leukemia & Lymphoma · doi:10.1080/10428190210176 · Cited by 61
Cited by
Dominy, S. S., Lynch, C., Ermini, F., Benedyk, M., Marczyk, A., Konradi, A., Nguyen, M., Haditsch, U., Raha, D., Griffin, C., et al. (2019) · Science Advances · doi:10.1126/sciadv.aau3333 · Cited by 1,966
Musso, D., & Gubler, D. J. (2016) · Clinical Microbiology Reviews · doi:10.1128/cmr.00072-15 · Cited by 1,469
Validation of Laboratory-Developed Molecular Assays for Infectious Diseases
Burd, E. M. (2010) · Clinical Microbiology Reviews · doi:10.1128/cmr.00074-09 · Cited by 550
Methods for the detection and identification of pathogenic bacteria: past, present, and future
Váradi, L., Luo, J. L., Hibbs, D. E., Perry, J. D., Anderson, R. J., Orenga, S., & Groundwater, P. W. (2017) · Chemical Society Reviews · doi:10.1039/c6cs00693k · Cited by 561
Stadhouders, R., Pas, S. D., Anber, J., Voermans, J., Mes, T. H. M., & Schutten, M. (2010) · The Journal of Molecular Diagnostics · doi:10.2353/jmoldx.2010.090035 · Cited by 399
Metadata
- Authors
- Espy, M. J., Uhl, J. R., Sloan, L. M., Buckwalter, S. P., Jones, M. F., Vetter, E. A., Yao, J. D. C., Wengenack, N. L., Rosenblatt, J. E., Cockerill, F. R., & Smith, T. F.
- Venue
- Clinical Microbiology Reviews
- Publisher
- American Society for Microbiology
- Published
- 2006-01
- Volume
- 19
- Issue
- 1
- Pages
- 165-256
- ISSN
- 0893-8512 (ISSN-L)
- 1098-6618
- OpenAlex
- W2158894328
- Cited by
- 1,318
- References
- 5
- FWCI
- 47.50
- Type
- journal article
- Language
- en
- Open access
- green
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- Espy, M. J., Uhl, J. R., Sloan, L. M., Buckwalter, S. P., Jones, M. F., Vetter, E. A., Yao, J. D. C., Wengenack, N. L., Rosenblatt, J. E., Cockerill, F. R., & Smith, T. F. (2006). Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing. Clinical Microbiology Reviews. https://doi.org/10.1128/cmr.19.1.165-256.2006
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
