The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Development of an Immunochromatographic Lateral-Flow Device for Rapid Serodiagnosis of Invasive Aspergillosis
Open accessAbstract
Aspergillus fumigatus is a cosmopolitan saprotrophic fungus that is second only to Candida species as a cause of invasive fungal infections in immunocompromised humans. Current immunodiagnostic tests for invasive aspergillosis (IA) are based on the detection of circulating galactomannan (GM) in a patient's serum by using a rat monoclonal antibody (MAb), EB-A2, that binds to tetra (1→5)-β- d -galactofuranoside, the immunodominant epitope in GM. The potential cross-reactivity of MAb EB-A2 with non- Aspergillus fungi, with contaminating GM in β-lactam antibiotics and foodstuffs, and with bacterial lipoteichoic acids has prompted efforts to discover non-GM antigens that can act as surrogate markers for the diagnosis of IA. This paper describes the development of a mouse MAb, JF5, that binds to a protein epitope present on an extracellular glycoprotein antigen secreted constitutively during the active growth of A. fumigatus . The MAb was used to develop an immunochromatographic lateral-flow device (LFD) for the rapid (15-min) detection of Aspergillus antigens in human serum. The test is highly specific, reacting with antigens from Aspergillus species but not with antigens from a large number of clinically important fungi, including Candida species, Cryptococcus neoformans , Fusarium solani , Penicillium marneffei , Pseudallescheria boydii , and Rhizopus oryzae . The LFD was able to detect circulating antigen in serum samples from patients suspected of having or shown to have IA on the basis of their clinical symptoms and results from tests for GM and fungal (1→3)-β- d -glucan. The ease of use of the LFD provides a diagnostic platform for the routine testing of vulnerable patients who have an elevated risk of IA.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Another MAb recognizing a galactofuranose-rich peptidoglycan molecule that circulates in serum and other biological fluids of patients with IA has been selected (124, 125).”
From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [124]
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Aspergillus fumigatus and Aspergillosis
Latgé, J.-P. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.2.310 · Cited by 2,307
Ribes, J. A., Vanover-Sams, C. L., & Baker, D. J. (2000) · Clinical Microbiology Reviews · doi:10.1128/cmr.13.2.236-301.2000 · Cited by 1,702
Denning, D. W. (1998) · Clinical Infectious Diseases · doi:10.1086/513943 · Cited by 1,493
Aspergillus fumigatus and Aspergillosis in 2019
Latgé, J.-P., & Chamilos, G. (2019) · Clinical Microbiology Reviews · doi:10.1128/cmr.00140-18 · Cited by 1,400
Webster, J., & Weber, R. (1971) · Cambridge University Press eBooks · doi:10.1017/cbo9780511809026 · Cited by 804
Trends in Mortality Due to Invasive Mycotic Diseases in the United States, 1980–1997
McNeil, M. M., Nash, S. L., Hajjeh, R. A., Phelan, M. A., Conn, L. A., Plikaytis, B. D., & Warnock, D. W. (2001) · Clinical Infectious Diseases · doi:10.1086/322606 · Cited by 657
Infections due to emerging and uncommon medically important fungal pathogens
Walsh, T. J., Groll, A. H., Hiemenz, J., Fleming, R. V., Roilides, E., & Anaissie, E. J. (2004) · Clinical Microbiology and Infection · doi:10.1111/j.1470-9465.2004.00839.x · Cited by 600
Pazos, C., Pontón, J., & del Palacio, A. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.1.299-305.2005 · Cited by 335
Stynen, D., Goris, A., Sarfati, J., & Latgé, J. P. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.2.497-500.1995 · Cited by 330
Research works printed but not linked: 1
- 10.1128/CMR.13.2.236 · DOI
Cited by
Sorted by citations
Aspergillus fumigatus and Aspergillosis in 2019
Latgé, J.-P., & Chamilos, G. (2019) · Clinical Microbiology Reviews · doi:10.1128/cmr.00140-18 · Cited by 1,400
Koehler, P. A., Bassetti, M., Chakrabarti, A., Chen, S. C. ‐., Colombo, A. L., Hoenigl, M., Климко, Н. Н., Lass‐Flörl, C., Oladele, R. O., Vinh, D. C., et al. (2020) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(20)30847-1 · Cited by 956
Diagnosis of invasive fungal infections: challenges and recent developments
Fang, W., Wu, J., Cheng, M., Zhu, X., Du, M., Chen, C., Liao, W., Zhi, K., & Pan, W. (2023) · Journal of Biomedical Science · doi:10.1186/s12929-023-00926-2 · Cited by 413
Molecular and Nonmolecular Diagnostic Methods for Invasive Fungal Infections
Arvanitis, M., Anagnostou, T., Fuchs, B. B., Caliendo, A. M., & Mylonakis, E. E. (2014) · Clinical Microbiology Reviews · doi:10.1128/cmr.00091-13 · Cited by 367
Hoenigl, M., Prattes, J., Spiess, B., Wagner, J., Prueller, F., Raggam, R. B., Posch, V., Duettmann, W., Hoenigl, K., Wölfler, A., et al. (2014) · Journal of Clinical Microbiology · doi:10.1128/jcm.00467-14 · Cited by 233
Self-citation
Metadata
- Authors
- Thornton, C. R.
- Affiliations
- University of Exeter (GB)
- Corresponding author
- Thornton, Christopher R.
- Venue
- Clinical and Vaccine Immunology
- Publisher
- American Society for Microbiology
- Published
- 2008-07
- Volume
- 15
- Issue
- 7
- Pages
- 1095-1105
- ISSN
- 1556-679X (ISSN-L)
- 1556-6811
- PMC
- PMC2446636
- OpenAlex
- W2131814154
- Cited by
- 226
- References
- 49
- Citations by year
- 2026: 10 · 2025: 8 · 2024: 9 · 2023: 21 · 2022: 14 · 2021: 13 · 2020: 14 · 2019: 20 · 2018: 21 · 2017: 16 · 2016: 20 · 2015: 22 · 2014: 14 · 2013: 11 · 2012: 6
- FWCI
- 1.55
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Clinical and Vaccine Immunology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
- Keywords
- invasive aspergillosis, Aspergillus fumigatus, serological diagnosis, rapid antigen detection, immunocompromised patients, galactomannan, β-glucan, diagnostic specificity
- MeSH terms
- Antibodies, Fungal, Antibodies, Monoclonal, Antibody Specificity, Epitopes, Antigens, Fungal, Aspergillosis, Aspergillus fumigatus, Cross Reactions, Humans, Periodic Acid, Pronase, Sensitivity and Specificity, Serologic Tests, Trypsin, Microscopy, Immunoelectron
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
