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Cited by 226Biosensors and Analytical DetectionAntifungal resistance and susceptibilitySARS-CoV-2 detection and testing

The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.

Development of an Immunochromatographic Lateral-Flow Device for Rapid Serodiagnosis of Invasive Aspergillosis

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Thornton, Christopher R.·2008·Clinical and Vaccine Immunology
DOI 10.1128/cvi.00068-08PMC PMC2446636
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Abstract

Aspergillus fumigatus is a cosmopolitan saprotrophic fungus that is second only to Candida species as a cause of invasive fungal infections in immunocompromised humans. Current immunodiagnostic tests for invasive aspergillosis (IA) are based on the detection of circulating galactomannan (GM) in a patient's serum by using a rat monoclonal antibody (MAb), EB-A2, that binds to tetra (1→5)-β- d -galactofuranoside, the immunodominant epitope in GM. The potential cross-reactivity of MAb EB-A2 with non- Aspergillus fungi, with contaminating GM in β-lactam antibiotics and foodstuffs, and with bacterial lipoteichoic acids has prompted efforts to discover non-GM antigens that can act as surrogate markers for the diagnosis of IA. This paper describes the development of a mouse MAb, JF5, that binds to a protein epitope present on an extracellular glycoprotein antigen secreted constitutively during the active growth of A. fumigatus . The MAb was used to develop an immunochromatographic lateral-flow device (LFD) for the rapid (15-min) detection of Aspergillus antigens in human serum. The test is highly specific, reacting with antigens from Aspergillus species but not with antigens from a large number of clinically important fungi, including Candida species, Cryptococcus neoformans , Fusarium solani , Penicillium marneffei , Pseudallescheria boydii , and Rhizopus oryzae . The LFD was able to detect circulating antigen in serum samples from patients suspected of having or shown to have IA on the basis of their clinical symptoms and results from tests for GM and fungal (1→3)-β- d -glucan. The ease of use of the LFD provides a diagnostic platform for the routine testing of vulnerable patients who have an elevated risk of IA.

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  1. 10.1128/CMR.13.2.236 · DOI

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Metadata

Authors
Thornton, C. R.
Affiliations
University of Exeter (GB)
Corresponding author
Thornton, Christopher R.
ORCID
Thornton, Christopher R.
Venue
Clinical and Vaccine Immunology
Publisher
American Society for Microbiology
Published
2008-07
Volume
15
Issue
7
Pages
1095-1105
ISSN
  • 1556-679X (ISSN-L)
  • 1556-6811
DOI
10.1128/cvi.00068-08
PMC
PMC2446636
OpenAlex
W2131814154
Cited by
226
References
49
Citations by year
2026: 10 · 2025: 8 · 2024: 9 · 2023: 21 · 2022: 14 · 2021: 13 · 2020: 14 · 2019: 20 · 2018: 21 · 2017: 16 · 2016: 20 · 2015: 22 · 2014: 14 · 2013: 11 · 2012: 6
FWCI
1.55
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Clinical and Vaccine Immunology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
Keywords
invasive aspergillosis, Aspergillus fumigatus, serological diagnosis, rapid antigen detection, immunocompromised patients, galactomannan, β-glucan, diagnostic specificity
MeSH terms
Antibodies, Fungal, Antibodies, Monoclonal, Antibody Specificity, Epitopes, Antigens, Fungal, Aspergillosis, Aspergillus fumigatus, Cross Reactions, Humans, Periodic Acid, Pronase, Sensitivity and Specificity, Serologic Tests, Trypsin, Microscopy, Immunoelectron

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