The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains
Open accessAbstract
The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7 - 10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7 - 10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7 - 10 mutants increases the accumulation of lipids on a cellular basis during nitrogen deprivation relative to that in the CC124 control as determined by conversion to fatty acid methyl esters. However, this increased level of lipid accumulation is energetically insufficient to completely offset the loss of cellular starch that is synthesized by CC124 during nitrogen deprivation. We therefore investigated acetate utilization and O 2 evolution to obtain further insights into the physiological adjustments utilized by the two starchless mutants in the absence of starch synthesis. The results demonstrate that both starchless mutants metabolize less acetate and have more severely attenuated levels of photosynthetic O 2 evolution than CC124, indicating that a decrease in overall anabolic processes is a significant physiological response in the starchless mutants during nitrogen deprivation. Interestingly, two independent sta7 - 10 : STA7 complemented strains exhibited significantly greater quantities of cellular starch and lipid than CC124 during acclimation to nitrogen deprivation. Moreover, the complemented strains synthesized significant quantities of starch even when cultured in nutrient-replete medium.
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Cites
Genetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
Self-citationDirect photosynthetic recycling of carbon dioxide to isobutyraldehyde
Atsumi, S., Higashide, W., & Liao, J. C. (2009) · Nature Biotechnology · doi:10.1038/nbt.1586 · Cited by 870
Aquatic phototrophs: efficient alternatives to land-based crops for biofuels
Dismukes, G. C., Carrieri, D., Bennette, N., Ananyev, G. M., & Posewitz, M. C. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.05.007 · Cited by 733 · cited 1×
“Microalgae are able to efficiently convert sunlight, water, and CO2 into a variety of products suitable for renewable energy applications, including H2, carbohydrates, and lipids (11, 12, 16, 21, 38, 41, 44).”
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Microalgae as a future food source
Torres-Tiji, Y., Fields, F. J., & Mayfield, S. P. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107536 · Cited by 559
Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology
Daboussi, F., Leduc, S., Maréchal, A., Dubois, G., Guyot, V., Perez-Michaut, C., Amato, A., Falciatore, A., Juillerat, A., Beurdeley, M., et al. (2014) · Nature Communications · doi:10.1038/ncomms4831 · Cited by 455
Li, J., Han, D., Wang, D., Ning, K., Jia, J., Wei, L., Jing, X., Huang, S., Chen, J., Li, Y., et al. (2014) · The Plant Cell · doi:10.1105/tpc.113.121418 · Cited by 412
Li, X., Moellering, E. R., Liu, B., Johnny, C., Fedewa, M., Sears, B. B., Kuo, M.-H., & Benning, C. (2012) · The Plant Cell · doi:10.1105/tpc.112.105106 · Cited by 320
Metabolism of acyl‐lipids in Chlamydomonas reinhardtii
Li‐Beisson, Y., Beisson, F., & Riekhof, W. (2015) · The Plant Journal · doi:10.1111/tpj.12787 · Cited by 310
Metadata
- Authors
- Work, V. H., Radakovits, R., Jinkerson, R. E., Meuser, J. E., Elliott, L. G., Vinyard, D. J., Laurens, L. M. L., Dismukes, G. C., & Posewitz, M. C.
- Venue
- Eukaryotic Cell
- Publisher
- American Society for Microbiology
- Published
- 2010-08
- Volume
- 9
- Issue
- 8
- Pages
- 1251-1261
- ISSN
- 1535-9778 (ISSN-L)
- 1535-9786
- OpenAlex
- W2153983368
- Cited by
- 353
- References
- 3
- FWCI
- 11.86
- Type
- journal article
- Language
- en
- Open access
- diamond
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- Work, V. H., Radakovits, R., Jinkerson, R. E., Meuser, J. E., Elliott, L. G., Vinyard, D. J., Laurens, L. M. L., Dismukes, G. C., & Posewitz, M. C. (2010). Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains. Eukaryotic Cell. https://doi.org/10.1128/ec.00075-10
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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