Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology
Open accessAbstract
Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.
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By genome editing, disruption of the gene UDP-glucose pyrophosphorylase resulted in a 45-fold TAG accumulation in P. tricornutum.
“By genome editing, the gene UDP-glucose pyrophosphorylase was disrupted and a 45-fold of TAG accumulation was found in P. tricornutum [223].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [223]
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“By genome editing, the gene UDP-glucose pyrophosphorylase was disrupted and a 45-fold of TAG accumulation was found in P. tricornutum [223].”
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- Authors
- Daboussi, F., Leduc, S., Maréchal, A., Dubois, G., Guyot, V., Perez-Michaut, C., Amato, A., Falciatore, A., Juillerat, A., Beurdeley, M., Voytas, D. F., Cavarec, L., & Duchateau, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Cellectis (United States) (US)
- Corresponding author
- Daboussi, Fayza
- ORCID
- Venue
- Nature Communications
- Published
- 2014-05-29
- Volume
- 5
- Issue
- 1
- Pages
- 3831-3831
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2160586543
- Cited by
- 455
- References
- 45
- Citations by year
- 2026: 23 · 2025: 34 · 2024: 28 · 2023: 43 · 2022: 43 · 2021: 40 · 2020: 38 · 2019: 48 · 2018: 43 · 2017: 41 · 2016: 42 · 2015: 25 · 2014: 6
- FWCI
- 12.54
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- Phaeodactylum tricornutum, diatoms, genome editing, meganucleases, TALE nucleases, targeted mutagenesis, triglyceride accumulation, lipid production, biofuel production, UDP-glucose pyrophosphorylase, lipid metabolism genes, selectable marker
- MeSH terms
- Base Sequence, Biotechnology, Chromatography, High Pressure Liquid, Endonucleases, Flow Cytometry, Genetic Engineering, Genotype, Lipids, Molecular Sequence Data, Triglycerides, Trans-Activators, Mutagenesis, Genome, Diatoms, Tandem Mass Spectrometry, DNA Breaks, Double-Stranded, Mutation Rate
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