Structure and Function of Glycosylated Tandem Repeats from Candida albicans Als Adhesins
Open accessAbstract
Tandem repeat (TR) regions are common in yeast adhesins, but their structures are unknown, and their activities are poorly understood. TR regions in Candida albicans Als proteins are conserved glycosylated 36-residue sequences with cell-cell aggregation activity (J. M. Rauceo, R. De Armond, H. Otoo, P. C. Kahn, S. A. Klotz, N. K. Gaur, and P. N. Lipke, Eukaryot. Cell 5:1664–1673, 2006). Ab initio modeling with either Rosetta or LINUS generated consistent structures of three-stranded antiparallel β-sheet domains, whereas randomly shuffled sequences with the same composition generated various structures with consistently higher energies. O- and N-glycosylation patterns showed that each TR domain had exposed hydrophobic surfaces surrounded by glycosylation sites. These structures are consistent with domain dimensions and stability measurements by atomic force microscopy (D. Alsteen, V. Dupres, S. A. Klotz, N. K. Gaur, P. N. Lipke, and Y. F. Dufrene, ACS Nano 3: 1677–1682, 2009) and with circular dichroism determination of secondary structure and thermal stability. Functional assays showed that the hydrophobic surfaces of TR domains supported binding to polystyrene surfaces and other TR domains, leading to nonsaturable homophilic binding. The domain structures are like “classic” subunit interaction surfaces and can explain previously observed patterns of promiscuous interactions between TR domains in any Als proteins or between TR domains and surfaces of other proteins. Together, the modeling techniques and the supporting data lead to an approach that relates structure and function in many kinds of repeat domains in fungal adhesins.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Previous studies show that variations in tandem repeat copy numbers within fungal adhesin proteins can affect their surface hydrophobicity, cell surface display, and protein shedding, ultimately influencing their functions.
“This agrees with previous studies showing that variations in tandem repeat copy numbers within fungal adhesin proteins can affect their surface hydrophobicity, cell surface display, and protein shedding, ultimately influencing their functions52,53.”
From Adhesin Als4112 promotes Candida auris skin colonization through interactions with keratinocytes and extracellular matrix proteins (2025) · cites this paper as [53]
Cites
VMD: visual molecular dynamics
Humphrey, W., Dalke, A., & Schulten, K. (1996) · Journal of Molecular Graphics · doi:10.1016/0263-7855(96)00018-5 · Cited by 66,952 · see citing works
The Anatomy and Taxonomy of Protein Structure
Richardson, J. S. (1981) · Advances in protein chemistry · doi:10.1016/s0065-3233(08)60520-3 · Cited by 3,378 · see citing works
Sreerama, N., & Woody, R. W. (2000) · Analytical Biochemistry · doi:10.1006/abio.2000.4880 · Cited by 2,997 · see citing works
Prediction of the Secondary Structure of Proteins from their Amino Acid Sequence
Chou, P. Y., & Fasman, G. D. (1979) · Advances in enzymology and related areas of molecular biology/Advances in enzymology and related subjects · doi:10.1002/9780470122921.ch2 · Cited by 2,917 · see citing works
GLYCAM06: A generalizable biomolecular force field. Carbohydrates
Kirschner, K. N., Yongye, A. B., Tschampel, S. M., González‐Outeiriño, J., Daniels, C. R., Foley, B. L., & Woods, R. J. (2007) · Journal of Computational Chemistry · doi:10.1002/jcc.20820 · Cited by 2,253 · see citing works
A Hot Spot of Binding Energy in a Hormone-Receptor Interface
Clackson, T., & Wells, J. A. (1995) · Science · doi:10.1126/science.7529940 · Cited by 1,987 · see citing works
Protein Structure Prediction Using Rosetta
Rohl, C. A., Strauss, C. E. M., Misura, K. M. S., & Baker, D. A. (2004) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(04)83004-0 · Cited by 1,711 · see citing works
[32] GOR method for predicting protein secondary structure from amino acid sequence
Garnier, J., Gibrat, J.-F., & Robson, B. (1996) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(96)66034-0 · Cited by 1,260 · see citing works
Evolution of pathogenicity and sexual reproduction in eight Candida genomes
Butler, G., Rasmussen, M. D., Lin, M. F., Santos, M. A. S., Sakthikumar, S., Munro, C. A., Rheinbay, E., Grabherr, M., Forche, A., Reedy, J. L., et al. (2009) · Nature · doi:10.1038/nature08064 · Cited by 1,065 · see citing works
JPred: a consensus secondary structure prediction server.
Cuff, J. A., Clamp, M. E., Siddiqui, A. S., Finlay, M., & Barton, G. J. (1998) · Bioinformatics · doi:10.1093/bioinformatics/14.10.892 · Cited by 1,004 · see citing works
Visualizing density maps with UCSF Chimera
Goddard, T. D., Huang, C. C., & Ferrin, T. E. (2006) · Journal of Structural Biology · doi:10.1016/j.jsb.2006.06.010 · Cited by 876 · see citing works
Cell Wall Architecture in Yeast: New Structure and New Challenges
Lipke, P. N., & Ovalle, R. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.15.3735-3740.1998 · Cited by 701 · see citing works
Self-citationIntragenic tandem repeats generate functional variability
Verstrepen, K. J., Jansen, A., Lewitter, F., & Fink, G. R. (2005) · Nature Genetics · doi:10.1038/ng1618 · Cited by 616 · see citing works
Computation and Analysis of Protein Circular Dichroism Spectra
Sreerama, N., & Woody, R. W. (2004) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(04)83013-1 · Cited by 530 · see citing works
Estimation of protein secondary structure and error analysis from circular dichroism spectra
van Stokkum, I. H. M., Spoelder, H. J. W., Bloemendal, M., van Grondelle, R., & Groen, F. C. A. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90396-q · Cited by 503 · see citing works
Overview of N- and O-linked oligosaccharide structures found in various yeast species
Gemmill, T. R., & Trimble, R. B. (1999) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/s0304-4165(98)00126-3 · Cited by 439 · see citing works
Hoyer, L. L., Green, C. B., Oh, S.-H., & Zhao, X. (2008) · Medical Mycology · doi:10.1080/13693780701435317 · Cited by 356 · see citing works
Complementary Adhesin Function in C. albicans Biofilm Formation
Nobile, C. J., Schneider, H. A., Nett, J. E., Sheppard, D. C., Filler, S. G., Andes, D. R., & Mitchell, A. P. (2008) · Current Biology · doi:10.1016/j.cub.2008.06.034 · Cited by 351 · see citing works
Almeida, R. S., Brunke, S., Albrecht, A., Thewes, S., Laue, M., Edwards, J. E., Filler, S. G., & Hube, B. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000217 · Cited by 330 · see citing works
Kapteyn, J. C., Hoyer, L. L., Hecht, J. E., Müller, W. H., Andel, A., Verkleij, A. J., Makarow, M., Van Den Ende, H., & Klis, F. M. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01729.x · Cited by 328 · see citing works
Functional and Structural Diversity in the Als Protein Family of Candida albicans
Sheppard, D. C., Yeaman, M. R., Welch, W. H., Phan, Q. T., Fu, Y., Ibrahim, A. S., Filler, S. G., Zhang, M., Waring, A. J., & Edwards, J. E. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m401929200 · Cited by 300 · see citing works
A Biochemical Guide to Yeast Adhesins: Glycoproteins for Social and Antisocial Occasions
Dranginis, A., Rauceo, J. M., Coronado, J. E., & Lipke, P. (2007) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00037-06 · Cited by 296 · see citing works
Self-citationKlotz, S. A., Chasin, B. S., Powell, B., Gaur, N. K., & Lipke, P. N. (2007) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2007.07.001 · Cited by 252 · see citing works
Self-citationRoles for glycosylation of cell surface receptors involved in cellular immune recognition
Rudd, P. M., Wormald, M. R., Stanfield, R. L., Huang, M., Mattsson, N., Speir, J. A., Gennaro, J. A. D., Fetrow, J. S., Dwek, R. A., & Wilson, I. A. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.3104 · Cited by 236 · see citing works
ALTERNATIVECANDIDA ALBICANSLIFESTYLES: Growth on Surfaces
Kumamoto, C. A., & Vinces, M. D. (2005) · Annual Review of Microbiology · doi:10.1146/annurev.micro.59.030804.121034 · Cited by 236 · see citing works
Origins of variation in the fungal cell surface
Verstrepen, K., Reynolds, T., & Fink, G. (2004) · Nature Reviews Microbiology · doi:10.1038/nrmicro927 · Cited by 215 · see citing works
Lipke, P. N., & Kurjan, J. (1992) · Microbiological Reviews · doi:10.1128/mr.56.1.180-194.1992 · Cited by 163 · see citing works
Self-citationYeast Cell Adhesion Molecules Have Functional Amyloid-Forming Sequences
Ramsook, C. B., Tan, C., Garcia, M. C., Fung, R., Soybelman, G., Henry, R., Litewka, A., O'Meally, S., Otoo, H., Khalaf, R. A., et al. (2009) · Eukaryotic Cell · doi:10.1128/ec.00068-09 · Cited by 156 · see citing works
Self-citationEngineering allosteric protein switches by domain insertion
Ostermeier, M. (2005) · Protein Engineering Design and Selection · doi:10.1093/protein/gzi048 · Cited by 149 · see citing works
Candida albicans Als Adhesins Have Conserved Amyloid-Forming Sequences
Otoo, H., Lee, K., Qiu, W.-G., & Lipke, P. (2007) · Eukaryotic Cell · doi:10.1128/ec.00309-07 · Cited by 139 · see citing works
Self-citationCandida albicans Als proteins mediate aggregation with bacteria and yeasts.
Klotz, S., Gaur, N., Armond, R. L. D., Sheppard, D., Khardori, N., Edwards, J., Lipke, P., & El-azizi, M. (2007) · Medical Mycology · doi:10.1080/13693780701299333 · Cited by 125 · see citing works
Self-citationThreonine-Rich Repeats Increase Fibronectin Binding in the <i>Candidaalbicans</i> Adhesin Als5p
Rauceo, J. M., De Armond, R., Otoo, H., Kahn, P. C., Klotz, S. A., Gaur, N. K., & Lipke, P. N. (2006) · Eukaryotic Cell · doi:10.1128/ec.00120-06 · Cited by 115 · cited 10× · see citing works
“The Ig-like regions can interact with diverse mammalian proteins, presumably in a way analogous to antibody-antigen binding, as has been shown in the homologous protein -agglutinin from Saccharomyces cerevisiae (8, 24, 26, 35).”
BackgroundInfluentialSelf-citationOh, S.-H., Cheng, G., Nuessen, J. A., Jajko, R., Yeater, K. M., Zhao, X., Pujol, C., Soll, D. R., & Hoyer, L. L. (2005) · Microbiology · doi:10.1099/mic.0.27680-0 · Cited by 108 · see citing works
Comparison of the effects of 2,2,2-trifluoroethanol on peptide and protein structure and function
Povey, J. F., Smales, C. M., Hassard, S., & Howard, M. J. (2006) · Journal of Structural Biology · doi:10.1016/j.jsb.2006.07.008 · Cited by 106 · see citing works
A cradle for new proteins: trigger factor at the ribosome
Maier, T., Ferbitz, L., Deuerling, E., & Ban, N. (2005) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2005.03.005 · Cited by 103 · see citing works
Structure-based Protocol for Identifying Mutations that Enhance Protein–Protein Binding Affinities
Sammond, D. W., Eletr, Z. M., Purbeck, C., Kimple, R. J., Siderovski, D. P., & Kuhlman, B. A. (2007) · Journal of Molecular Biology · doi:10.1016/j.jmb.2007.05.096 · Cited by 102 · see citing works
Unfolding individual Als5p adhesion proteins on live cells
Alsteens, D., Dupres, V., Klotz, S., Gaur, N., Lipke, P., & Dufrêne, Y. (2009) · ACS Nano · doi:10.1021/nn900078p · Cited by 95 · see citing works
Self-citationGaur, N., Klotz, S., & Henderson, R. L. (1999) · Infection and Immunity · doi:10.1128/iai.67.11.6040-6047.1999 · Cited by 91 · see citing works
Self-citationDegenerate Peptide Recognition by Candida albicans Adhesins Als5p and Als1p
Klotz, S., Gaur, N., Lake, D., Chan, V., Rauceo, J. M., & Lipke, P. (2004) · Infection and Immunity · doi:10.1128/iai.72.4.2029-2034.2004 · Cited by 89 · see citing works
Self-citationZhang, N.-X., Harrex, A., Holland, B., Fenton, L., Cannon, R., & Schmid, J. (2003) · Genome Research · doi:10.1101/gr.1024903 · Cited by 79 · see citing works
Functional analysis of the <i>Candida albicans ALS1</i> gene product
Loza, L., Fu, Y., Ibrahim, A. S., Sheppard, D. C., Filler, S. G., & Edwards, J. E. (2004) · Yeast · doi:10.1002/yea.1111 · Cited by 78 · see citing works
Global Cell Surface Conformational Shift Mediated by a Candida albicans Adhesin
Rauceo, J. M., Gaur, N. K., Lee, K.-G., Edwards, J. E., Klotz, S. A., & Lipke, P. N. (2004) · Infection and Immunity · doi:10.1128/iai.72.9.4948-4955.2004 · Cited by 68 · see citing works
Self-citationThe GPI-modified proteins Pga59 and Pga62 of Candida albicans are required for cell wall integrity
Moreno‐Ruiz, E., Ortu, G., de Groot, P. W. J., Cottier, F., Loussert-Fonta, C., Prévost, M., de Koster, C. G., Klis, F. M., Goyard, S., & d’Enfert, C. (2009) · Microbiology · doi:10.1099/mic.0.028902-0 · Cited by 62 · see citing works
Microbial biofilms: e pluribus unum
Nobile, C. J., & Mitchell, A. P. (2007) · Current Biology · doi:10.1016/j.cub.2007.02.035 · Cited by 59 · see citing works
Conserved Processes and Lineage-Specific Proteins in Fungal Cell Wall Evolution
Coronado, J. E., Mneimneh, S., Epstein, S. L., Qiu, W., & Lipke, P. N. (2007) · Eukaryotic Cell · doi:10.1128/ec.00044-07 · Cited by 59 · see citing works
Self-citationZhao, X.-M., Oh, S.-H., Jajko, R., Diekema, D., Pfaller, M., Pujol, C., Soll, D., & Hoyer, L. (2007) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2007.05.004 · Cited by 49 · see citing works
Candida biofilms: is adhesion sexy?
Soll, D. (2008) · Current Biology · doi:10.1016/j.cub.2008.07.014 · Cited by 46 · see citing works
Ab Initio Protein Folding Using LINUS
Srinivasan, R., Fleming, P. J., & Rose, G. D. (2004) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(04)83003-9 · Cited by 37 · see citing works
Gaur, N., & Klotz, S. (2004) · Microbiology · doi:10.1099/mic.0.26738-0 · Cited by 35 · see citing works
Self-citationA CD2‐Based Model of Yeast a‐Agglutinin Elucidates Solution Properties and Binding Characteristics
Grigorescu, A., Chen, M., Zhao, H., Kahn, P., & Lipke, P. (2000) · IUBMB Life · doi:10.1080/713803692 · Cited by 14 · see citing works
Self-citationZhao, H. F., Chen, M.-H., Shen, Z., Kahn, P. C., & Lipke, P. N. (2001) · Protein Science · doi:10.1110/ps.41701 · Cited by 12 · see citing works
Self-citationHazen, K. C., Singleton, D. R., & Masuoka, J. (2007) · Glycobiology · doi:10.1093/glycob/cwm080 · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Candida albicans Als3, a multifunctional adhesin and invasin
Liu, Y., & Filler, S. G. (2011) · Eukaryotic Cell · doi:10.1128/ec.00279-10 · Cited by 354 · see citing works
Diversity, biogenesis and function of microbial amyloids
Blanco, L. P., Evans, M. L., Smith, D. R., Badtke, M. P., & Chapman, M. R. (2012) · Trends in Microbiology · doi:10.1016/j.tim.2011.11.005 · Cited by 320 · see citing works
Adhesins in human fungal pathogens: glue with plenty of stick.
de Groot, P. W. J., Bader, O., de Boer, A. D., Weig, M., & Chauhan, N. (2013) · Eukaryotic Cell · doi:10.1128/ec.00364-12 · Cited by 319 · see citing works
Latgé, J. (2010) · Cellular Microbiology · doi:10.1111/j.1462-5822.2010.01474.x · Cited by 295 · see citing works
Hoyer, L. L., & Cota, E. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00280 · Cited by 216 · see citing works
Force-induced formation and propagation of adhesion nanodomains in living fungal cells
Alsteens, D., Garcia, M. C., Lipke, P. N., & Dufrêne, Y. F. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1013893107 · Cited by 189 · see citing works
Self-citationChoosing the right lifestyle: adhesion and development inSaccharomyces cerevisiae
Brückner, S., & Mösch, H. (2011) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2011.00275.x · Cited by 164 · see citing works
A Role for Amyloid in Cell Aggregation and Biofilm Formation
Garcia, M. C., Lee, J. T., Ramsook, C. B., Alsteens, D., Dufrêne, Y., & Lipke, P. (2011) · PLoS ONE · doi:10.1371/journal.pone.0017632 · Cited by 128 · see citing works
Self-citationStrengthening relationships: amyloids create adhesion nanodomains in yeasts
Lipke, P. N., Garcia, M. C., Alsteens, D., Ramsook, C. B., Klotz, S. A., & Dufrêne, Y. F. (2012) · Trends in Microbiology · doi:10.1016/j.tim.2011.10.002 · Cited by 104 · see citing works
Self-citationWhat We Do Not Know about Fungal Cell Adhesion Molecules
Lipke, P. (2018) · Journal of Fungi · doi:10.3390/jof4020059 · Cited by 81 · see citing works
Self-citationFlocculation protein structure and cell–cell adhesion mechanism in Saccharomyces cerevisiae
Goossens, K. V. Y., & Willaert, R. (2010) · Biotechnology Letters · doi:10.1007/s10529-010-0352-3 · Cited by 92 · see citing works
Salgado, P., Yan, R., Taylor, J. D., Burchell, L., Jones, R., Hoyer, L., Matthews, S., Simpson, P., & Cota, E. (2011) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.1103496108 · Cited by 91 · see citing works
<i>O</i> -Mannosylation in Candida albicans Enables Development of Interkingdom Biofilm Communities
Dutton, L. C., Nobbs, A. H., Jepson, K., Jepson, M. A., Vickerman, M. M., Aqeel Alawfi, S., Munro, C. A., Lamont, R. J., & Jenkinson, H. F. (2014) · mBio · doi:10.1128/mbio.00911-14 · Cited by 75 · see citing works
On the evolution of fungal and yeast cell walls
Xie, X., & Lipke, P. N. (2010) · Yeast · doi:10.1002/yea.1787 · Cited by 64 · see citing works
Self-citationAmyloid-Like β-Aggregates as Force-Sensitive Switches in Fungal Biofilms and Infections
Lipke, P., Klotz, S., Dufrêne, Y., Jackson, D., & Garcia-Sherman, M. C. (2017) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00035-17 · Cited by 55 · see citing works
Self-citationCovalently linked wall proteins in ascomycetous fungi
Klis, F. M., Brul, S., & de Groot, P. W. J. (2009) · Yeast · doi:10.1002/yea.1747 · Cited by 62 · see citing works
Bamford, C. V., Nobbs, A., Barbour, M., Lamont, R., & Jenkinson, H. (2015) · Microbiology · doi:10.1099/mic.0.083378-0 · Cited by 62 · see citing works
Nanoscale analysis of caspofungin-induced cell surface remodelling in Candida albicans
El-Kirat-Chatel, S., Beaussart, A., Alsteens, D., Jackson, D., Lipke, P., & Dufrêne, Y. (2012) · Nanoscale · doi:10.1039/c2nr33215a · Cited by 56 · see citing works
Self-citationDonohue, D. S., Ielasi, F., Goossens, K. V. Y., & Willaert, R. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2011.07676.x · Cited by 54 · see citing works
Single-cell force spectroscopy of Als-mediated fungal adhesion
Alsteens, D., Beaussart, A., Derclaye, S., El‐Kirat‐Chatel, S., Park, H. R., Lipke, P. N., & Dufrêne, Y. F. (2013) · Analytical Methods · doi:10.1039/c3ay40473k · Cited by 50 · see citing works
Self-citationInsight into the Antiadhesive Effect of Yeast Wall Protein 1 of Candida albicans
Granger, B. L. (2012) · Eukaryotic Cell · doi:10.1128/ec.00026-12 · Cited by 48 · see citing works
Opportunistic yeast pathogen Candida spp.: Secreted and membrane-bound virulence factors
Lim, S. J., Ali, M. S. M., Sabri, S., Noor, N. D. M., Salleh, A. B., & Oslan, S. N. (2021) · Medical Mycology · doi:10.1093/mmy/myab053 · Cited by 41 · see citing works
Lagree, K., & Mitchell, A. P. (2017) · Microbiology Spectrum · doi:10.1128/microbiolspec.funk-0024-2016 · Cited by 40 · see citing works
Role of cell surface hydrophobicity in Candida albicans biofilm
Bujdáková, H., Didiášová, M., Drahovská, H., & Černáková, L. (2013) · Open Life Sciences · doi:10.2478/s11535-013-0136-y · Cited by 40 · see citing works
Secreted Aspartyl Proteases Family: A Perspective Review on the Regulation Of Fungal Pathogenesis
Kulshrestha, A., & Gupta, P. (2023) · Future Microbiology · doi:10.2217/fmb-2022-0143 · Cited by 40 · see citing works
Marc, G., Araniciu, C., Oniga, S. D., Vlase, L., Pı̂rnău, A., Duma, M. N., Marutescu, L. G., Chifiriuc, M. C., & Oniga, O. (2018) · Molecules · doi:10.3390/molecules23102522 · Cited by 40 · see citing works
Role of Force-Sensitive Amyloid-Like Interactions in Fungal Catch Bonding and Biofilms
Chan, C. X. J., & Lipke, P. (2014) · Eukaryotic Cell · doi:10.1128/ec.00068-14 · Cited by 31 · see citing works
Self-citationAn Amyloid Core Sequence in the Major Candida albicans Adhesin Als1p Mediates Cell-Cell Adhesion
Ho, V., Herman-Bausier, P., Shaw, C., Conrad, K., Garcia-Sherman, M. C., Draghi, J., Dufrêne, Y., Lipke, P., & Rauceo, J. M. (2019) · mBio · doi:10.1128/mbio.01766-19 · Cited by 28 · see citing works
Self-citationOrmsby, M. J., Akinbobola, A., & Quilliam, R. S. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.163093 · Cited by 33 · see citing works
Zhao, G., Lyu, J., Veniaminova, N. A., Zarnowski, R., Mattos, E., Johnson, C. J., Quintanilla, D., Hautau, H., Hold, L. A., Xu, B., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-60876-1 · Cited by 32 · see citing works
“This agrees with previous studies showing that variations in tandem repeat copy numbers within fungal adhesin proteins can affect their surface hydrophobicity, cell surface display, and protein shedding, ultimately in fl uencing their functions 52,53 .”
Goossens, K. V. Y., & Willaert, R. (2012) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2011.00766.x · Cited by 37 · see citing works
Peptide Detection of Fungal Functional Amyloids in Infected Tissue
Garcia, M. C., Lysak, N., Filonenko, A., Richards, H., Sobonya, R. E., Klotz, S. A., & Lipke, P. N. (2014) · PLoS ONE · doi:10.1371/journal.pone.0086067 · Cited by 29 · see citing works
Self-citationPadder, S. A., Ramzan, A., Tahir, I., Rehman, R. U., & Shah, A. H. (2022) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2021.1935447 · Cited by 27 · see citing works
Sticking to the Subject: Multifunctionality in Microbial Adhesins
Lipke, P., & Ragonis-Bachar, P. (2023) · Journal of Fungi · doi:10.3390/jof9040419 · Cited by 25 · see citing works
Self-citationParallel expansion and divergence of an adhesin family in pathogenic yeasts
Smoak, R. A., Snyder, L. F., Fassler, J. S., & He, B. Z. (2023) · GENETICS · doi:10.1093/genetics/iyad024 · Cited by 25 · see citing works
Adhesins in Opportunistic Fungal Pathogens
Zordan, R., & Cormack, B. (2012) · ASM Press eBooks · doi:10.1128/9781555817176.ch16 · Cited by 28 · see citing works
Zhao, X.-M., Oh, S.-H., Coleman, D. A., & Hoyer, L. (2011) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.2010.00769.x · Cited by 22 · see citing works
Between Amyloids and Aggregation Lies a Connection with Strength and Adhesion
Lipke, P., Ramsook, C. B., Garcia-Sherman, M. C., Jackson, D., Chan, C. X. J., Bois, M., & Klotz, S. (2014) · New Journal of Science · doi:10.1155/2014/815102 · Cited by 22 · see citing works
Self-citationBeaussart, A., Brandhorst, T. T., Dufrêne, Y. F., & Klein, B. S. (2015) · mBio · doi:10.1128/mbio.01403-15 · Cited by 18 · see citing works
Ahmad, M. F., Yadav, B., Kumar, P., Puri, A., Mazumder, M., Ali, A., Gourinath, S., Muthuswami, R., & Komath, S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0035305 · Cited by 16 · see citing works
Analysis of flocculins in Ashbya gossypii reveals FIG2 regulation by TEC1
Grünler, A., Walther, A., Lämmel, J., & Wendland, J. (2010) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2010.04.001 · Cited by 13 · see citing works
Does Candida albicans Als5p Amyloid Play a Role in Commensalism in Caenorhabditis elegans?
Bois, M., Singh, S., Samlalsingh, A., Lipke, P., & Garcia, M. C. (2013) · Eukaryotic Cell · doi:10.1128/ec.00020-13 · Cited by 14 · see citing works
Self-citationThe Cell Wall: Glycoproteins, Remodeling, and Regulation
Munro, C. A., & Richard, M. L. (2014) · ASM Press eBooks · doi:10.1128/9781555817176.ch14 · Cited by 13 · see citing works
Structure, dynamics and domain organization of the repeat protein Cin1 from the apple scab fungus
Mesarich, C. H., Schmitz, M., Tremouilhac, P., McGillivray, D. J., Templeton, M. D., & Dingley, A. J. (2012) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2012.06.015 · Cited by 13 · see citing works
Zoppo, M., Luca, M. D., Villarreal, S. N., Poma, N. V., Barrasa, M. I., Bottai, D., Vyas, V. K., & Tavanti, A. (2019) · Future Microbiology · doi:10.2217/fmb-2019-0168 · Cited by 13 · see citing works
Structural and Functional Characterization of PA14/Flo5-Like Adhesins From Komagataella pastoris
Kock, M. A., Brückner, S., Wozniak, N., Maestre‐Reyna, M., Veelders, M., Schlereth, J., Mösch, H., & Essen, L. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02581 · Cited by 12 · see citing works
Ibe, C., Walker, L. A., Gow, N. A. R., & Munro, C. A. (2017) · Springer eBooks · doi:10.1007/978-3-319-50409-4_16 · Cited by 10 · see citing works
Yoshimoto, S., Aoki, S., Ohara, Y., Ishikawa, M., Suzuki, A., Linke, D., Lupas, A. N., & Hori, K. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1095057 · Cited by 9 · see citing works
Lagree, K., & Mitchell, A. P. (2017) · ASM Press eBooks · doi:10.1128/9781555819583.ch42 · Cited by 9 · see citing works
Lim, S. J., Noor, N. D. M., Sabri, S., Ali, M. S. M., Salleh, A. B., & Oslan, S. N. (2024) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.2023.2300757 · Cited by 8 · see citing works
von Ranke, N. V., Bello, M. L., Cabral, L., Castro, H., & Rodrigues, C. (2018) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.2017.1417159 · Cited by 7 · see citing works
Functional Amyloids in Adhesion of Non-albicans Candida Species
Garcia-Sherman, M. C., Hamid, S. A., Jackson, D., Thomas, J. D., & Lipke, P. (2025) · Pathogens · doi:10.3390/pathogens14080723 · Cited by 5 · see citing works
Self-citationEncounters with Mammalian Cells: Survival Strategies of Candida Species
Vylkova, S., & Lorenz, M. (2012) · ASM Press eBooks · doi:10.1128/9781555817176.ch17 · Cited by 8 · see citing works
Evolution and Genomics of the PathogenicCandidaSpecies Complex
Butler, G., Lorenz, M., & Gow, N. A. R. (2012) · Wiley eBooks · doi:10.1002/9781118308165.ch21 · Cited by 3 · see citing works
Candida auris adhesins, specifically Als3, a key target to understand and mitigate
Lashine, Y., Hamdy, R., Dakalbab, S., Lazreg, I. K., Aldhaheri, F., Chin, S.-P., Hashim, N., & Soliman, S. S. M. (2025) · Mycologia · doi:10.1080/00275514.2025.2536345 · Cited by 3 · see citing works
Application of Nanotechnologies in Studying Yeast Structure in Candida
Savin, N. A., Колмогоров, В., Timoshenko, R. V., Klyachko, N. L., Parkhomenko, Y. N., Salikhov, S. V., Majouga, A. G., Erofeev, A. S., & Gorelkin, P. V. (2021) · Nanobiotechnology Reports · doi:10.1134/s263516762104011x · Cited by 2 · see citing works
Zhao, G., Lyu, J., Veniaminova, N. A., Zarnowski, R., Mattos, E., Johnson, C. J., Quintanilla, D., Hautau, H., Hold, L. A., Xu, B., et al. (2025) · doi:10.1101/2025.02.13.637978 · Cited by 2 · see citing works
Nanomechanics of Yeast Surfaces Revealed by AFM
Dague, E., Beaussart, A., & Alsteens, D. (2012) · Nanoscience and technology · doi:10.1007/978-3-642-25414-7_7
Alsteens, D. (2013) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.2001773
(2010) · Yeast · doi:10.1002/yea.1719
A Role of Yeast Adhesin Amyloids in Force-Dependent Adhesion and Biofilm Formation
Chan, C. X. J. (2014) · CUNY Academic Works (City University of New York)
In Silico Analysis and Expression Profiling of Adhesin-Like Flocculins in Trichosporon asahii
dos Santos, M. J. M., Quinto, D. D. C., Gagliano, G. D. S., Francisco, E., & Padovan, A. C. (2026) · Mycopathologia · doi:10.1007/s11046-025-01045-2
- Authors
- Frank, A. T., Ramsook, C. B., Otoo, H. N., Tan, C., Soybelman, G., Rauceo, J. M., Gaur, N. K., Klotz, S. A., & Lipke, P. N.
- Affiliations
- University of Michigan (US)
- City University of New York (US)
- University of Arizona (US)
- Venue
- Eukaryotic Cell
- Publisher
- American Society for Microbiology
- Published
- 2010-03
- Volume
- 9
- Issue
- 3
- Pages
- 405-414
- ISSN
- 1535-9778 (ISSN-L)
- 1535-9786
- PMC
- PMC2837987
- OpenAlex
- W2120471906
- Cited by
- 63
- References
- 52
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 1 · 2023: 5 · 2021: 3 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 1 · 2015: 1 · 2014: 9 · 2013: 5 · 2012: 7
- FWCI
- 3.08
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Eukaryotic Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- CUNY Academic Works (City University of New York) (submittedVersion)
- Funders
- National Science Foundation
- National Institutes of Health (2s07rr005357-30)
- National Institute of General Medical Sciences (S06 GM 070758, GM 55230, GM 083756, R01GM055230, SC1GM083756, R29GM055230)
- National Center for Research Resources (RR 05357)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- Candida albicans, tandem repeat domain, glycosylation, protein structure, antiparallel beta-sheet, homophilic binding, cell-cell aggregation, protein-protein interaction surfaces, O-glycosylation, N-glycosylation, structure-function relationship, fungal adhesins
- MeSH terms
- Amino Acid Sequence, Candida albicans, Circular Dichroism, Disaccharides, Enzyme-Linked Immunosorbent Assay, Fibronectins, Fungal Proteins, Glycosylation, Mannosides, Models, Molecular, Molecular Sequence Data, Peptide Fragments, Polystyrenes, Protein Binding, Protein Denaturation, Serine, Threonine, Cell Adhesion Molecules, Sequence Homology, Amino Acid, Protein Structure, Secondary, Protein Structure, Tertiary, Protein Renaturation, Lectins, Protein Interaction Domains and Motifs, Molecular Dynamics Simulation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review