The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii
Open accessAbstract
The unicellular green alga Chlamydomonas reinhardtii is a valuable model for studying metal metabolism in a photosynthetic background. A search of the Chlamydomonas expressed sequence tag database led to the identification of several components that form a copper-dependent iron assimilation pathway related to the high-affinity iron uptake pathway defined originally for Saccharomyces cerevisiae. They include a multicopper ferroxidase (encoded by Fox1), an iron permease (encoded by Ftr1), a copper chaperone (encoded by Atx1), and a copper-transporting ATPase. A cDNA,Fer1, encoding ferritin for iron storage also was identified. Expression analysis demonstrated that Fox1 and Ftr1 were coordinately induced by iron deficiency, as were Atx1 and Fer1, although to lesser extents. In addition, Fox1 abundance was regulated at the posttranscriptional level by copper availability. Each component exhibited sequence relationship with its yeast, mammalian, or plant counterparts to various degrees; Atx1 of C. reinhardtii is also functionally related with respect to copper chaperone and antioxidant activities. Fox1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate, for the first time, a role for copper in iron assimilation in a photosynthetic species. Nevertheless, growth of C. reinhardtii under copper- and iron-limiting conditions showed that, unlike the situation in yeast and mammals, where copper deficiency results in a secondary iron deficiency, copper-deficient Chlamydomonas cells do not exhibit symptoms of iron deficiency. We propose the existence of a copper-independent iron assimilation pathway in this organism.
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Cites
Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter
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Cited by
Copper in plants: acquisition, transport and interactions
Yruela, I. (2009) · Functional Plant Biology · doi:10.1071/fp08288 · Cited by 912
The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation
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Self-citationThe Trace Metal Composition of Marine Phytoplankton
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In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
Greco, M., Chiappetta, A., Bruno, L., & Bitonti, M. B. (2012) · Journal of Experimental Botany · doi:10.1093/jxb/err313 · Cited by 538
Metadata
- Authors
- La Fontaine, S., Quinn, J. M., Nakamoto, S. S., Page, M. D., Göhre, V., Moseley, J. L., Kropat, J., & Merchant, S.
- Venue
- Eukaryotic Cell
- Publisher
- American Society for Microbiology
- Published
- 2002-10
- Volume
- 1
- Issue
- 5
- Pages
- 736-757
- ISSN
- 1535-9778 (ISSN-L)
- 1535-9786
- OpenAlex
- W2055172046
- Cited by
- 194
- References
- 11
- FWCI
- 9.40
- Type
- journal article
- Language
- en
- Open access
- diamond
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- La Fontaine, S., Quinn, J. M., Nakamoto, S. S., Page, M. D., Göhre, V., Moseley, J. L., Kropat, J., & Merchant, S. (2002). Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell. https://doi.org/10.1128/ec.1.5.736-757.2002
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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