Temperature-Regulated Microcolony Formation by Burkholderia pseudomallei Requires pilA and Enhances Association with Cultured Human Cells
Open accessAbstract
Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease that is endemic to Northern Australia and Southeast Asia and is acquired from soil or water. Adherence of B. pseudomallei 08 to cultured cells increases dramatically following prior growth at 30 degrees C or less compared to that following prior growth at 37 degrees C. Here, we show that this occurs almost entirely as the result of microcolony formation (bacterium-bacterium interactions) following growth at 27 degrees C but not at 37 degrees C, which considerably enhances bacterial association with eukaryotic cells. Further, we demonstrate that the type IVA pilin-encoding gene, pilA, is essential for microcolony development by B. pseudomallei 08, and thus optimum association with eukaryotic cells, but is not required for direct adherence (bacterium-cell interactions). In contrast, although the B. pseudomallei genome sequence strain, K96243, also contains transcriptionally active pilA, microcolony formation rarely occurs following growth at either 27 degrees C or 37 degrees C and cell association occurs significantly less than with strain 08. Analysis of pilA transcription in 08 identified that pilA is dramatically upregulated under microcolony-forming conditions, viz., growth at low temperature, and association with eukaryotic cells; the pattern of transcription of pilA in K96243 differed from that in 08. Our study also suggests that biofilm formation by B. pseudomallei 08 and K96243 on polyvinylchloride is not mediated by pilA. Adherence and microcolony formation, and pilA transcription, vary between strains, consistent with known genomic variation in B. pseudomallei, and these phenotypes may be relevant to colonization from the environment.
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Microcolony and biofilm formation are two separate processes.
“Thus, microcolony and biofilm formation are two separate processes [27].”
From Bacterial autoaggregation (2018) · cites this paper as [27]
In Burkholderia pseudomallei, microcolony formation is mediated by type IV pili in a temperature-regulated manner; in some strains type IV pili are not needed for biofilm development, and in some conditions the lack of type IV pili can increase biofilm formation.
“Another example is Burkholderia pseudomallei, where microcolony formation is mediated by type IV pili in a temperature-regulated manner [27]. However, in some B. pseudomallei strains, type IV pili are not needed for biofilm development, and in some conditions the lack of type IV pili can increase biofilm formation.”
From Bacterial autoaggregation (2018) · cites this paper as [27]
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- Authors
- Boddey, J. A., Flegg, C. P., Day, C. J., Beacham, I. R., & Peak, I. R.
- Affiliations
- Institute for Glycomics
- School of Medical Science, Griffith University, Gold Coast, Queensland, Australia
- Griffith University (AU)
- Corresponding author
- Beacham, Ifor R.
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2006-09
- Volume
- 74
- Issue
- 9
- Pages
- 5374-5381
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC1594825
- OpenAlex
- W2169327644
- Cited by
- 51
- References
- 51
- Citations by year
- 2026: 2 · 2025: 1 · 2023: 1 · 2022: 3 · 2021: 8 · 2020: 3 · 2019: 1 · 2018: 2 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 3
- FWCI
- 2.21
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Griffith Research Online (submittedVersion)
- Funders
- Medical Research Council
- National Health and Medical Research Council
- Topics
- Health Sciences › Medicine › Epidemiology › Burkholderia infections and melioidosis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacillus and Francisella bacterial research
- Keywords
- Burkholderia pseudomallei, melioidosis, microcolony formation, Pila, bacterial adhesion, low-temperature growth, biofilm formation, strain differences, bacterial colonization
- MeSH terms
- Bacterial Adhesion, Cells, Cultured, Humans, Polyvinyl Chloride, Temperature, Transcription, Genetic, Genetic Variation, Gene Expression Regulation, Bacterial, Burkholderia pseudomallei, Biofilms, Fimbriae Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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