The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
The Streptococcuspneumoniae Capsule Inhibits Complement Activity and Neutrophil Phagocytosis by Multiple Mechanisms
Open accessAbstract
The Streptococcuspneumoniae capsule is vital for virulence and may inhibit complement activity and phagocytosis. However, there are only limited data on the mechanisms by which the capsule affects complement and the consequences for S. pneumoniae interactions with phagocytes. Using unencapsulated serotype 2 and 4 S. pneumoniae mutants, we have confirmed that the capsule has several effects on complement activity. The capsule impaired bacterial opsonization with C3b/iC3b by both the alternative and classical complement pathways and also inhibited conversion of C3b bound to the bacterial surface to iC3b. There was increased binding of the classical pathway mediators immunoglobulin G (IgG) and C-reactive protein (CRP) to unencapsulated S. pneumoniae, indicating that the capsule could inhibit classical pathway complement activity by masking antibody recognition of subcapsular antigens, as well as by inhibiting CRP binding. Cleavage of serum IgG by the enzyme IdeS reduced C3b/iC3b deposition on all of the strains, but there were still marked increases in C3b/iC3b deposition on unencapsulated TIGR4 and D39 strains compared to encapsulated strains, suggesting that the capsule inhibits both IgG-mediated and IgG-independent complement activity against S. pneumoniae. Unencapsulated strains were more susceptible to neutrophil phagocytosis after incubation in normal serum, normal serum treated with IdeS, complement-deficient serum, and complement-deficient serum treated with IdeS or in buffer alone, suggesting that the capsule inhibits phagocytosis mediated by Fcγ receptors, complement receptors, and nonopsonic receptors. Overall, these data show that the S. pneumoniae capsule affects multiple aspects of complement- and neutrophil-mediated immunity, resulting in a profound inhibition of opsonophagocytosis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Walport, M. J. (2001) · New England Journal of Medicine · doi:10.1056/nejm200104053441406 · Cited by 2,927
WHO estimates of the causes of death in children
Bryce, J., Boschi-Pinto, C., Shibuya, K., & Black, R. E. (2005) · The Lancet · doi:10.1016/s0140-6736(05)71877-8 · Cited by 2,096
C3d of complement as a molecular adjuvant: Bridging innate and acquired immunity
Dempsey, P. W., Allison, M. E. D., Akkaraju, S., Goodnow, C. C., & Fearon, D. T. (1996) · Science · doi:10.1126/science.271.5247.348 · Cited by 1,197
Human C-reactive protein: expression, structure, and function
Volanakis, J. (2001) · Molecular Immunology · doi:10.1016/s0161-5890(01)00042-6 · Cited by 926
Genetic Analysis of the Capsular Biosynthetic Locus from All 90 Pneumococcal Serotypes
Bentley, S. D., Aanensen, D. M., Mavroidi, A., Saunders, D., Rabbinowitsch, E., Collins, M., Donohoe, K., Harris, D., Murphy, L., Quail, M. A., et al. (2006) · PLoS Genetics · doi:10.1371/journal.pgen.0020031 · Cited by 759
Neth, O., Jack, D. L., Dodds, A. W., Holzel, H., Klein, N. J., & Turner, M. W. (2000) · Infection and Immunity · doi:10.1128/iai.68.2.688-693.2000 · Cited by 561
IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G
von Pawel-Rammingen, U. (2002) · The EMBO Journal · doi:10.1093/emboj/21.7.1607 · Cited by 473
Briles, D. E., Nahm, M., Schroer, K., Davie, J., Baker, P., Kearney, J., & Barletta, R. (1981) · The Journal of experimental medicine · doi:10.1084/jem.153.3.694 · Cited by 464
Hammerschmidt, S., Wolff, S., Hocke, A., Rosseau, S., Müller, E., & Rohde, M. (2005) · Infection and Immunity · doi:10.1128/iai.73.8.4653-4667.2005 · Cited by 420
Brown, J. S., Hussell, T., Gilliland, S. M., Holden, D. W., Paton, J. C., Ehrenstein, M. R., Walport, M. J., & Botto, M. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.012669199 · Cited by 370
Self-citation
Cited by
Pneumococcal Capsules and Their Types: Past, Present, and Future
Geno, K. A., Gilbert, G. L., Song, J. Y., Skovsted, I. C., Klugman, K. P., Jones, C., Konradsen, H. B., & Nahm, M. H. (2015) · Clinical Microbiology Reviews · doi:10.1128/cmr.00024-15 · Cited by 732
Pathogenesis and Pathophysiology of Pneumococcal Meningitis
Mook-Kanamori, B. B., Geldhoff, M., van der Poll, T., & van de Beek, D. (2011) · Clinical Microbiology Reviews · doi:10.1128/cmr.00008-11 · Cited by 492
Complement receptors and their role in leukocyte recruitment and phagocytosis
Vandendriessche, S., Cambier, S., Proost, P., & Marques, P. E. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.624025 · Cited by 178
Skov Sørensen, U. B., Yao, K., Yang, Y., Tettelin, H., & Kilian, M. (2016) · mBio · doi:10.1128/mbio.01844-16 · Cited by 100
Rendueles, O., Garcia-Garcerà, M., Néron, B., Touchon, M., & Rocha, E. P. C. (2017) · PLOS Pathogens · doi:10.1371/journal.ppat.1006525 · Cited by 89
Metadata
- Authors
- Hyams, C., Camberlein, E., Cohen, J. M., Bax, K., & Brown, J. S.
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2010-02
- Volume
- 78
- Issue
- 2
- Pages
- 704-715
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- OpenAlex
- W2119458127
- Cited by
- 458
- References
- 46
- FWCI
- 6.11
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The Streptococcuspneumoniae Capsule Inhibits Complement Activity and Neutrophil Phagocytosis by Multiple Mechanisms. https://app.cochat.ai/papers/10.1128/iai.00881-09
- The paper
- Hyams, C., Camberlein, E., Cohen, J. M., Bax, K., & Brown, J. S. (2010). The Streptococcuspneumoniae Capsule Inhibits Complement Activity and Neutrophil Phagocytosis by Multiple Mechanisms. Infection and Immunity. https://doi.org/10.1128/iai.00881-09
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
