Cited by 89Heat shock proteins researchFungal and yeast genetics researchAntifungal resistance and susceptibility

Structure and regulation of the HSP90 gene from the pathogenic fungus Candida albicans

Open access
Swoboda, R K, Bertram, G, Budge, S, Gooday, G W, Gow, N A, Brown, A J·1995·Infection and Immunity

Abstract

Candida albicans HSP90 sequences were isolated by screening cDNA and genomic libraries with a probe derived from the Saccharomyces cerevisiae homolog, HSP82, which encodes a member of the heat shock protein 90 family of molecular chaperones. Identical sequences were obtained for the 2,197-bp overlap of the cDNA and gene sequences, which were derived from C. albicans 3153A and ATCC 10261, respectively. The C. albicans HSP90 gene contained no introns, and it showed strong homology (61 to 79% identity) to HSP90 sequences from other fungi, vertebrates, and plants. The C-terminal portion of the predicted Hsp90 amino acid sequence was identical to the 47-kDa protein which is thought to be immunoprotective during C. albicans infections (R. C. Matthews, J. Med. Microbiol. 36:367-370, 1992), confirming that this protein represents the C-terminal portion of the 81-kDa Hsp90 protein. Quantitative Northern (RNA) analyses revealed that C. albicans HSP90 mRNA was heat shock inducible and that its levels changed during batch growth, with its maximum levels being reached during the mid-exponential growth phase. HSP90 mRNA levels increased transiently during the yeast-to-hyphal transition but did not correlate directly with germ tube production per se. These data do not exclude a role for Hsp90 in the dimorphic transition. Southern blotting revealed only one HSP90 locus in the diploid C. albicans genome. Repeated attempts to disrupt both alleles and generate a homozygous C. albicans delta hsp90/delta hsp90 null mutant were unsuccessful. These observations suggest the existence of a single HSP90 locus which is essential for viability in C. albicans.

Integrity signals

Registry

Crossref · 10.1128/iai.63.11.4506-4514.1995

Cites

51 of 51 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Swoboda, R. K., Bertram, G., Budge, S., Gooday, G. W., Gow, N. A., & Brown, A. J.
Affiliations
University of Aberdeen (GB)
Venue
Infection and Immunity
Published
1995-11
Volume
63
Issue
11
Pages
4506-4514
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
OpenAlex
W1930313341
Cited by
89
References
51
Citations by year
2023: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 1 · 2016: 4 · 2015: 1 · 2014: 4 · 2013: 5 · 2012: 5
FWCI
2.11
Type
journal article
Language
en
Open access
Locations
  • Infection and Immunity (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
Candida albicans, HSP90, molecular chaperones, gene structure, gene expression regulation, heat shock induction, yeast-to-hypha transition, dimorphic transition, germ tube formation, fungal pathogens
MeSH terms
Amino Acid Sequence, Base Sequence, Candida albicans, Cloning, Molecular, DNA, Fungal, Fungal Proteins, Genes, Fungal, Hot Temperature, Molecular Sequence Data, Morphogenesis, RNA, Fungal, Gene Expression Regulation, Fungal, Mutagenesis, Insertional, Sequence Alignment, Sequence Homology, Amino Acid, DNA Primers, DNA, Complementary, HSP90 Heat-Shock Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review