Cited by 295Redox biology and oxidative stressBacterial Genetics and BiotechnologySulfur Compounds in Biology

Escherichia coli peptide methionine sulfoxide reductase gene: regulation of expression and role in protecting against oxidative damage

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Moskovitz, J, Rahman, M A, Strassman, J, Yancey, S O, Kushner, S R, Brot, N, Weissbach, H·1995·Journal of Bacteriology

Abstract

The Escherichia coli peptide methionine sulfoxide reductase gene (msrA) encodes a single-subunit polypeptide of 212 amino acid residues (M. A. Rahman, H. Nelson, H. Weissbach, and N. Brot, J. Biol. Chem. 267:15549-15551, 1992). RNA blot analysis showed that the gene is transcribed into an mRNA of about 850 nucleotides. The promoter region was characterized, and the transcription initiation site was identified by primer extension. The synthesis of the MsrA protein increased about threefold in a growth-phase-dependent fashion. In an attempt to define the in vivo role of msrA, a chromosomal disruption was constructed. This mutant was more sensitive to oxidative stress, suggesting that oxidation of methionine in proteins plays an important role in oxidative damage.

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Crossref · 10.1128/jb.177.3.502-507.1995

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  • Methionine sulfoxide reductase knockout bacteria are sensitized to the cell killing effects of hydrogen peroxide.

    “Significantly, methionine sulfoxide reductase knockout bacteria are sensitized to the cell killing effects of hydrogen peroxide [33,34].”

    From Discrimination of Methionine Sulfoxide and Sulfone by Human Neutrophil Elastase (2021) · cites this paper as [33]

    The Abstract reports that an E. coli msrA chromosomal-disruption mutant was more sensitive to oxidative stress. The passages in our copy do not specify hydrogen peroxide or show that the increased sensitivity was measured as cell killing, so those details are not supported. (LLM, conf=0.84)

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Authors
Moskovitz, J., Rahman, M. A., Strassman, J., Yancey, S. O., Kushner, S. R., Brot, N., & Weissbach, H.
Affiliations
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
Venue
Journal of Bacteriology
Published
1995-02
Volume
177
Issue
3
Pages
502-507
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
OpenAlex
W2107508146
Cited by
294
References
31
Citations by year
2026: 3 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 7 · 2020: 4 · 2019: 9 · 2018: 8 · 2017: 4 · 2016: 9 · 2015: 9 · 2014: 3 · 2013: 10 · 2012: 7
FWCI
2.85
Type
journal article
Language
en
Open access
Locations
  • Journal of Bacteriology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • KU ScholarWorks (University of Kansas) (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
Keywords
Escherichia coli, msrA gene, methionine sulfoxide reductase, gene expression regulation, promoter analysis, transcription start sites, oxidative stress sensitivity, oxidative damage protection
MeSH terms
Base Sequence, Escherichia coli, Genes, Bacterial, Methionine, Molecular Sequence Data, Mutation, Oxidoreductases, RNA, Messenger, Gene Expression Regulation, Bacterial, Oxidative Stress, Methionine Sulfoxide Reductases

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