The DOI matches its Crossref record; 60 of the 62 references it cites were found in a registry or an index.
Loss of the Plasma Membrane-Bound Protein Gas1p in Saccharomyces cerevisiae Results in the Release of β1,3-Glucan into the Medium and Induces a Compensation Mechanism To Ensure Cell Wall Integrity
Open accessAbstract
Deletion of GAS1/GGP1/CWH52 results in a lower β-glucan content of the cell wall and swollen, more spherical cells (L. Popolo, M. Vai, E. Gatti, S. Porello, P. Bonfante, R. Balestrini, and L. Alberghina, J. Bacteriol. 175:1879–1885, 1993; A. F. J. Ram, S. S. C. Brekelmans, L. J. W. M. Oehlen, and F. M. Klis, FEBS Lett. 358:165–170, 1995). We show here that gas1 Δ cells release β1,3-glucan into the medium. Western analysis of the medium proteins with β1,3-glucan- and β1,6-glucan-specific antibodies showed further that at least some of the released β1,3-glucan was linked to protein as part of a β1,3-glucan–β1,6-glucan–protein complex. These data indicate that Gas1p might play a role in the retention of β1,3-glucan and/or β-glucosylated proteins. Interestingly, the defective incorporation of β1,3-glucan in the cell wall was accompanied by an increase in chitin and mannan content in the cell wall, an enhanced expression of cell wall protein 1 (Cwp1p), and an increase in β1,3-glucan synthase activity, probably caused by the induced expression of Fks2p. It is proposed that the cell wall weakening caused by the loss of Gas1p induces a set of compensatory reactions to ensure cell integrity.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Our functional analyses demonstrate that CHS3 is crucial during hyphal growth in the absence of Phr1p and support the notion that Chs3p is the major compensatory chitin synthase both in cell wall stress and HWS in fungi [21, 28, 47, 62, 63].”
From Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling (2016) · cites this paper as [63]
In the citing paper
“Our functional analyses demonstrate that CHS3 is crucial during hyphal growth in the absence of Phr1p and support the notion that Chs3p is the major compensatory chitin synthase both in cell wall stress and HWS in fungi [21, 28, 47, 62, 63].”
From Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling (2016) · cites this paper as [63]
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,311
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,512
Transformation of intact yeast cells treated with alkali cations
Ito, H., Fukuda, Y., Murata, K., & Kimura, A. (1983) · Journal of Bacteriology · doi:10.1128/jb.153.1.163-168.1983 · Cited by 7,518
Cold Spring Harbor Laboratory; Cold Spring Harbor, NY
Cavalier-Smith, T. (1980) · FEBS Letters · doi:10.1016/0014-5793(80)80323-1 · Cited by 6,317
Hoffman, C. S., & Winston, F. (1987) · Gene · doi:10.1016/0378-1119(87)90131-4 · Cited by 2,492
Methods in Yeast Genetics: A Laboratory Course Manual
Rose, M. D., Winston, F., & Hieter, P. (1990) · Medical Entomology and Zoology · Cited by 1,894
A simple and efficient method for direct gene deletion inSaccharomyces cerevisiae
Baudin‐Baillieu, A., Ozier-Kalogéropoulos, O., DENOUEL, A., Lacroute, F., & Cullin, C. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.14.3329 · Cited by 1,268
Schibler, U., Wyler, T., & Hagenbüchle, O. (1975) · Journal of Molecular Biology · doi:10.1016/0022-2836(75)90217-x · Cited by 1,187
Construction of a set of convenient saccharomyces cerevisiae strains that are isogenic to S288C
Winston, F., Dollard, C. A., & Ricupero‐Hovasse, S. L. (1995) · Yeast · doi:10.1002/yea.320110107 · Cited by 949
Architecture of the Yeast Cell Wall
KOLLÁR, R., Reinhold, B. B., Petráková, E., Yeh, H. J. C., Ashwell, G., Drgonová, J., Kapteyn, J. C., Klis, F. M., & Cabib, E. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.28.17762 · Cited by 643
Self-citationReview: Cell wall assembly in yeast
Klis, F. M. (1994) · Yeast · doi:10.1002/yea.320100702 · Cited by 552
Self-citationKamada, Y., Jung, U. S., Piotrowski, J., & Levin, D. E. (1995) · Genes & Development · doi:10.1101/gad.9.13.1559 · Cited by 488
Identification of Yeast Rho1p GTPase as a Regulatory Subunit of 1,3-β-Glucan Synthase
Qadota, H., Python, C. P., Inoue, S., Arisawa, M., Anraku, Y., Zheng, Y., Watanabe, T., Levin, D. E., & Ohya, Y. (1996) · Science · doi:10.1126/science.272.5259.279 · Cited by 445
Differential Expression and Function of Two Homologous Subunits of Yeast 1,3-β-D-Glucan Synthase
Mazur, P., Morin, N. R., Baginsky, W. F., El-Sherbeini, M., Clemas, J. A., NIELSEN, J. B. K., & Foor, F. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.10.5671 · Cited by 425
Self-citationDouglas, C. M., Foor, F., Marrinan, J. A., Morin, N., Nielsen, J. B., Dahl, A. M., Mazur, P., Baginsky, W., Li, W., & el-Sherbeini, M. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.26.12907 · Cited by 401
Self-citationThe structure of a β-(1→3)-d-glucan from yeast cell walls
Manners, D. J., Masson, A. J., & Patterson, J. C. (1973) · Biochemical Journal · doi:10.1042/bj1350019 · Cited by 384
Berben, G., Dumont, J. E., Gilliquet, V., Bolle, P. A., & Hilger, F. (1991) · Yeast · doi:10.1002/yea.320070506 · Cited by 339
Rho1p, a Yeast Protein at the Interface Between Cell Polarization and Morphogenesis
Drgonová, J., Drgon, T., Tanaka, K., KOLLÁR, R., Chen, G.-C., Ford, R. A., Chan, C. S. M., Takai, Y., & Cabib, E. (1996) · Science · doi:10.1126/science.272.5259.277 · Cited by 336
The structure of a β-(1→6)- d -glucan from yeast cell walls
Manners, D. J., Masson, A. J., Patterson, J. C., Björndal, H., & Lindberg, B. (1973) · Biochemical Journal · doi:10.1042/bj1350031 · Cited by 320
Ram, A. F. J., Wolters, A., ten Hoopen, R., & Klis, F. M. (1994) · Yeast · doi:10.1002/yea.320100804 · Cited by 300
Self-citationActivation of Yeast Protein Kinase C by Rho1 GTPase
Kamada, Y., Qadota, H., Python, C. P., Anraku, Y., Ohya, Y., & Levin, D. E. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.16.9193 · Cited by 280
Architecture of the Yeast Cell Wall
KOLLÁR, R., Petráková, E., Ashwell, G., Robbins, P. W., & Cabib, E. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.3.1170 · Cited by 266
The PMT gene family: protein O‐glycosylation in Saccharomyces cerevisiae is vital.
Gentzsch, M., & Tanner, W. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00961.x · Cited by 250
The glucanase‐soluble mannoproteins limit cell wall porosity in Saccharomyces cerevisiae
Nobel, J. G. D., Klis, F. M., Priem, J., Munnik, T., & ENDE, H. V. D. (1990) · Yeast · doi:10.1002/yea.320060606 · Cited by 248
Self-citationKapteyn, J. C., Montijn, R. C., Vink, E., de la Cruz, J., Llobell, A., Douwes, J. E., Shimoi, H., Lipke, P. N., & Klis, F. M. (1996) · Glycobiology · doi:10.1093/glycob/6.3.337 · Cited by 240
Self-citationIdentification of three mannoproteins in the cell wall of Saccharomyces cerevisiae
van der Vaart, J. M., P., L. H., Chapman, J. W., Klis, F. M., & Verrips, C. T. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.11.3104-3110.1995 · Cited by 240
Self-citationPopolo, L., Gilardelli, D., Bonfante, P., & Vai, M. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.2.463-469.1997 · Cited by 226
Nuoffer, C., Jenö, P., Conzelmann, A., & Riezman, H. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.1.27 · Cited by 220
Lu, C., Montijn, R. C., Brown, J. L., Klis, F. M., Kurjan, J., Bussey, H., & Lipke, P. N. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.128.3.333 · Cited by 204
Self-citationCharacterization and Gene Cloning of 1,3-beta-D-Glucan Synthase from Saccharomyces Cerevisiae
Inoue, S., Takewakt, N., Takasuka, T., Mio, T., Adachi, M., Fujii, Y., Miyamoto, C., Arisawa, M., Furuichi, Y., & Watanabe, T. (1995) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1995.0845d.x · Cited by 201
Activity of 1,3-β-D-Glucan Synthase Requires the GTP-binding Protein Rho1
Mazur, P., & Baginsky, W. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.24.14604 · Cited by 196
Self-citationOzols, J. (1990) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(90)82046-5 · Cited by 189
Garrett-Engele, P. W., Moilanen, B., & Cyert, M. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.8.4103 · Cited by 187
Kapteyn, J. C., Ram, A. F. J., Groos, E. M., KOLLÁR, R., Montijn, R. C., ENDE, H. V. D., Llobell, A., Cabib, E., & Klis, F. M. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.20.6279-6284.1997 · Cited by 181
Self-citationTargeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae
Schreuder, M. P., Brekelmans, S. S. C., ENDE, H. V. D., & Klis, F. M. (1993) · Yeast · doi:10.1002/yea.320090410 · Cited by 159
Self-citationFleet, G. H., & Manners, D. J. (1976) · Journal of General Microbiology · doi:10.1099/00221287-94-1-180 · Cited by 152
de la Cruz, J., Pintor‐Toro, J. A., Benítez, T., & Llobell, A. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.7.1864-1871.1995 · Cited by 149
Douwes, J. E., Doekes, G., Montijn, R. C., Heederik, D. J. J., & Brunekreef, B. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.9.3176-3182.1996 · Cited by 133
Self-citationRam, A. F. J., Brekelmans, S. S. C., Oehlen, L. J. W. M., & Klis, F. M. (1995) · FEBS Letters · doi:10.1016/0014-5793(94)01418-z · Cited by 132
Self-citationIncreased Cell Wall Porosity in Saccharomyces cerevisiae after Treatment with Dithiothreitol or EDTA
Nobel, J. G. D., Dijkers, C., Hooijberg, E., & Klis, F. M. (1989) · Microbiology · doi:10.1099/00221287-135-7-2077 · Cited by 130
Self-citationMontijn, R. C., Rinsum, J. V., van Schagen, F. A., & Klis, F. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32172-5 · Cited by 130
Self-citationA Saccharomyces cerevisiae mutant with echinocandin-resistant 1,3-beta-D-glucan synthase
Douglas, C. M., Marrinan, J. A., Li, W., & Kurtz, M. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.18.5686-5696.1994 · Cited by 129
Popolo, L., Vai, M., Gatti, E., Porello, S. L., Bonfante, P., Balestrini, R., & Alberghina, L. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.7.1879-1885.1993 · Cited by 113
Kapteyn, J. C., Montijn, R. C., Dijkgraaf, G. J. P., ENDE, H. V. D., & Klis, F. M. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.13.3788-3792.1995 · Cited by 99
Self-citationCloning and characterization of KNR4, a yeast gene involved in (1,3)-beta-glucan synthesis.
Hong, Z., Mann, P. A., Brown, N. H., Tran, L. E., Shaw, K. J., Hare, R. S., & DiDomenico, B. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.2.1017 · Cited by 95
Eng, W.-K., Faucette, L. F., McLaughlin, M. M., Cafferkey, R., Koltin, Y., Morris, R. A., Young, P. R., Johnson, R. K., & Livi, G. P. (1994) · Gene · doi:10.1016/0378-1119(94)90633-5 · Cited by 86
Vai, M., Gatti, E., Lacaná, E., Popolo, L., & Alberghina, L. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98888-5 · Cited by 83
Nuoffer, C., Jenö, P., Conzelmann, A., & Riezman, H. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.1.27-37.1991 · Cited by 75
Castro, C., Ribas, J. C., Valdivieso, M. ‐., Varona, R., del Rey, F., & Duran, A. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.20.5732-5739.1995 · Cited by 67
Jiang, B., Sheraton, J., Ram, A. F. J., Dijkgraaf, G. J. P., Klis, F. M., & Bussey, H. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.4.1162-1171.1996 · Cited by 66
Self-citationPringle, J. R., Broach, J. R., & Jones, E. W. (1997) · Medical Entomology and Zoology · Cited by 63
Calcineurin-dependent growth of an FK506- and CsA-hypersensitive mutant of Saccharomyces cerevisiae
Parent, S. A., NIELSEN, J. B. K., Morin, N. R., Chrebet, G. L., Ramadan, N., Dahl, A. M., Hsu, M. J., Bostian, K. A., & Foor, F. (1993) · Journal of General Microbiology · doi:10.1099/00221287-139-12-2973 · Cited by 62
Shimoi, H., Iimura, Y., & OBATA, T. (1995) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a124907 · Cited by 60
3 Biogenesis of Yeast Wall and Surface ComponentsPreprint
Orlean, P. (1997) · Cold Spring Harbor Monograph Archive · doi:10.1101/087969364.21c.229 · Cited by 36
Hong, Z., Mann, P. A., Shaw, K. J., & DiDomenico, B. (1994) · Yeast · doi:10.1002/yea.320100810 · Cited by 29
Gooday, G. W., & Schofield, D. A. (1995) · Canadian Journal of Botany · doi:10.1139/b95-233 · Cited by 28
Cloning and Characterization of KNR4 , a Yeast Gene Involved in (1,3)-β-Glucan Synthesis
Hong, Z., Mann, P. A., Brown, N. H., Tran, L. E., Shaw, K. J., Hare, R. S., & DiDomenico, B. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.2.1017-1025.1994 · Cited by 28
Research works printed but not linked: 2
- Ausubel F. Brent R. Kingston R. Moore D. Seidman J. Smith J. Struhl K. Current protocols in molecular biology (CD-ROM). 1995 John Wiley & Sons Inc. New York N.Y
- Fleet G. H. Cell walls The yeasts Rose A. H. Harrison J. S. 4 1991 199 277 Academic Press, Inc. New York, N.Y
Cited by
Sorted by citations
Cell Wall Integrity Signaling in Saccharomyces cerevisiae
Levin, D. E. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.2.262-291.2005 · Cited by 1,136
Dynamics of cell wall structure in Saccharomyces cerevisiae
Klis, F. M., Mol, P. C., Hellingwerf, K. J., & Brul, S. (2002) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2002.tb00613.x · Cited by 1,007
Self-citationCell Wall Assembly in Saccharomyces cerevisiae
Lesage, G., & Bussey, H. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00038-05 · Cited by 849
Cell wall construction in Saccharomyces cerevisiae
Klis, F. M., Boorsma, A., & de Groot, P. W. J. (2006) · Yeast · doi:10.1002/yea.1349 · Cited by 806
Self-citationCell Wall Architecture in Yeast: New Structure and New Challenges
Lipke, P. N., & Ovalle, R. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.15.3735-3740.1998 · Cited by 701
Metadata
- Authors
- Ram, A. F. J., Kapteyn, J. C., Montijn, R. C., Caro, L. H. P., Douwes, J. E., Baginsky, W., Mazur, P., van den Ende, H., & Klis, F. M.
- Affiliations
- University of Amsterdam (NL)
- <!--label omitted: 1-->Institute of Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, 1098 SM Amsterdam,1 and
- Wageningen University & Research (NL)
- Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1998-03-15
- Volume
- 180
- Issue
- 6
- Pages
- 1418-1424
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC107039
- OpenAlex
- W2152455126
- Cited by
- 200
- References
- 62
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 7 · 2020: 6 · 2019: 10 · 2018: 4 · 2017: 5 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 6 · 2012: 7
- FWCI
- 4.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- UvA-DARE (University of Amsterdam) (publishedVersion)
- Socio-Environmental Systems Modeling (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- plasma membrane proteins, cell wall integrity, chitin, mannan, Saccharomyces cerevisiae
- MeSH terms
- Carbohydrates, Cell Wall, Chitin, Cloning, Molecular, DNA, Fungal, Fungal Proteins, Glucans, Glucosyltransferases, Glycoproteins, Mannans, Membrane Glycoproteins, Membrane Proteins, Plasmids, Recombination, Genetic, RNA, Fungal, Saccharomyces cerevisiae, Blotting, Western, Gene Expression, Polymerase Chain Reaction, Culture Media, Conditioned, Sequence Deletion, Saccharomyces cerevisiae Proteins, beta-Glucans, Carbohydrate Metabolism
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
