The DOI matches its Crossref record; 60 of the 62 references it cites were found in a registry or an index.
Loss of the Plasma Membrane-Bound Protein Gas1p in Saccharomyces cerevisiae Results in the Release of β1,3-Glucan into the Medium and Induces a Compensation Mechanism To Ensure Cell Wall Integrity
Open accessAbstract
Deletion of GAS1/GGP1/CWH52 results in a lower β-glucan content of the cell wall and swollen, more spherical cells (L. Popolo, M. Vai, E. Gatti, S. Porello, P. Bonfante, R. Balestrini, and L. Alberghina, J. Bacteriol. 175:1879–1885, 1993; A. F. J. Ram, S. S. C. Brekelmans, L. J. W. M. Oehlen, and F. M. Klis, FEBS Lett. 358:165–170, 1995). We show here that gas1 Δ cells release β1,3-glucan into the medium. Western analysis of the medium proteins with β1,3-glucan- and β1,6-glucan-specific antibodies showed further that at least some of the released β1,3-glucan was linked to protein as part of a β1,3-glucan–β1,6-glucan–protein complex. These data indicate that Gas1p might play a role in the retention of β1,3-glucan and/or β-glucosylated proteins. Interestingly, the defective incorporation of β1,3-glucan in the cell wall was accompanied by an increase in chitin and mannan content in the cell wall, an enhanced expression of cell wall protein 1 (Cwp1p), and an increase in β1,3-glucan synthase activity, probably caused by the induced expression of Fks2p. It is proposed that the cell wall weakening caused by the loss of Gas1p induces a set of compensatory reactions to ensure cell integrity.
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“Our functional analyses demonstrate that CHS3 is crucial during hyphal growth in the absence of Phr1p and support the notion that Chs3p is the major compensatory chitin synthase both in cell wall stress and HWS in fungi [21, 28, 47, 62, 63].”
From Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling (2016) · cites this paper as [63]
In the citing paper
“Our functional analyses demonstrate that CHS3 is crucial during hyphal growth in the absence of Phr1p and support the notion that Chs3p is the major compensatory chitin synthase both in cell wall stress and HWS in fungi [21, 28, 47, 62, 63].”
From Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling (2016) · cites this paper as [63]
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Cited by
Sorted by citations
Cell Wall Integrity Signaling in Saccharomyces cerevisiae
Levin, D. E. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.2.262-291.2005 · Cited by 1,136
Dynamics of cell wall structure in Saccharomyces cerevisiae
Klis, F. M., Mol, P. C., Hellingwerf, K. J., & Brul, S. (2002) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2002.tb00613.x · Cited by 1,007
Self-citationCell Wall Assembly in Saccharomyces cerevisiae
Lesage, G., & Bussey, H. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00038-05 · Cited by 849
Cell wall construction in Saccharomyces cerevisiae
Klis, F. M., Boorsma, A., & de Groot, P. W. J. (2006) · Yeast · doi:10.1002/yea.1349 · Cited by 806
Self-citationCell Wall Architecture in Yeast: New Structure and New Challenges
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Metadata
- Authors
- Ram, A. F. J., Kapteyn, J. C., Montijn, R. C., Caro, L. H. P., Douwes, J. E., Baginsky, W., Mazur, P., van den Ende, H., & Klis, F. M.
- Affiliations
- University of Amsterdam (NL)
- <!--label omitted: 1-->Institute of Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, 1098 SM Amsterdam,1 and
- Wageningen University & Research (NL)
- Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1998-03-15
- Volume
- 180
- Issue
- 6
- Pages
- 1418-1424
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC107039
- OpenAlex
- W2152455126
- Cited by
- 200
- References
- 62
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 7 · 2020: 6 · 2019: 10 · 2018: 4 · 2017: 5 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 6 · 2012: 7
- FWCI
- 4.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- UvA-DARE (University of Amsterdam) (publishedVersion)
- Socio-Environmental Systems Modeling (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- plasma membrane proteins, cell wall integrity, chitin, mannan, Saccharomyces cerevisiae
- MeSH terms
- Carbohydrates, Cell Wall, Chitin, Cloning, Molecular, DNA, Fungal, Fungal Proteins, Glucans, Glucosyltransferases, Glycoproteins, Mannans, Membrane Glycoproteins, Membrane Proteins, Plasmids, Recombination, Genetic, RNA, Fungal, Saccharomyces cerevisiae, Blotting, Western, Gene Expression, Polymerase Chain Reaction, Culture Media, Conditioned, Sequence Deletion, Saccharomyces cerevisiae Proteins, beta-Glucans, Carbohydrate Metabolism
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