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Cited by 573

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli

Open access
Murphy, Kenan C.·1998·Journal of Bacteriology

Abstract

Replacement of Escherichia coli ’s RecBCD function with phage λ’s Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The Δ recBCD ::P lac -red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.

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Crossref · 10.1128/jb.180.8.2063-2071.1998

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Metadata

Authors
Murphy, K. C.
Venue
Journal of Bacteriology
Published
1998-04-15
Volume
180
Issue
8
Pages
2063-2071
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
OpenAlex
W1884874540
Cited by
573
References
24
FWCI
8.15
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli. https://app.cochat.ai/papers/10.1128/jb.180.8.2063-2071.1998
The paper
Murphy, K. C. (1998). Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli. Journal of Bacteriology. https://doi.org/10.1128/jb.180.8.2063-2071.1998

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.