The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Overexpression of the MexEF-OprN Multidrug Efflux System Affects Cell-to-Cell Signaling in Pseudomonas aeruginosa
Open accessAbstract
Intrinsic and acquired antibiotic resistance of the nosocomial pathogen Pseudomonas aeruginosa is mediated mainly by the expression of several efflux pumps of broad substrate specificity. Here we report that nfxC type mutants, overexpressing the MexEF-OprN efflux system, produce lower levels of extracellular virulence factors than the susceptible wild type. These include pyocyanin, elastase, and rhamnolipids, three factors controlled by the las and rhl quorum-sensing systems of P. aeruginosa . In agreement with these observations are the decreased transcription of the elastase gene lasB and the rhamnosyltransferase genes rhlAB measured in nfxC type mutants. Expression of the lasR and rhlR regulator genes was not affected in the nfxC type mutant. In contrast, transcription of the C4-homoserine lactone (C4-HSL) autoinducer synthase gene rhlI was reduced by 50% in the nfxC type mutant relative to that in the wild type. This correlates with a similar decrease in C4-HSL levels detected in supernatants of the nfxC type mutant. Transcription of an rhlAB - lacZ fusion could be partially restored by the addition of synthetic C4-HSL and Pseudomonas quinolone signal (PQS). It is proposed that the MexEF-OprN efflux pump affects intracellular PQS levels.
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Cited by
The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria
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Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence
Moradali, M. F., Ghods, S., & Rehm, B. H. A. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00039 · Cited by 1,537
Efflux-mediated antimicrobial resistance
Poole, K. (2005) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dki171 · Cited by 1,082
The role of bacterial biofilms in chronic infections
Bjarnsholt, T. (2013) · APMIS · doi:10.1111/apm.12099 · Cited by 1,080
Beceiro, A., Tomás, M., & Bou, G. (2013) · Clinical Microbiology Reviews · doi:10.1128/cmr.00059-12 · Cited by 1,070
Metadata
- Authors
- Köhler, T., van Delden, C., Curty, L. K., Hamzehpour, M. M., & Pechere, J.-C.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2001-09-15
- Volume
- 183
- Issue
- 18
- Pages
- 5213-5222
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2111678991
- Cited by
- 287
- References
- 54
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- green
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- Köhler, T., van Delden, C., Curty, L. K., Hamzehpour, M. M., & Pechere, J.-C. (2001). Overexpression of the MexEF-OprN Multidrug Efflux System Affects Cell-to-Cell Signaling in Pseudomonas aeruginosa. Journal of Bacteriology. https://doi.org/10.1128/jb.183.18.5213-5222.2001
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