The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Global Transposon Mutagenesis and Essential Gene Analysis of Helicobacter pylori
Open accessAbstract
We have constructed a genome-saturating mutant library of the human gastric pathogen Helicobacter pylori . Microarray tracking of transposon mutants (MATT) allowed us to map the position of 5,363 transposon mutants in our library. While we generally found insertions well distributed throughout the genome, 344 genes had no detectable transposon insertions, and this list is predicted to be highly enriched for essential genes. Comparison to the essential gene set of other bacteria revealed a surprisingly limited overlap with all organisms tested (11%), while 55% were essential in some organisms but not others. We independently verified the essentiality of several gene products, including an HtrA family serine protease, a hypothetical protein with putative phospholipase D activity, and a riboflavin specific deaminase. A limited screen for motility mutants allowed us to estimate that 4.5% of the genome is dedicated to this virulence-associated phenotype.
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Cites
The complete genome sequence of the gastric pathogen Helicobacter pylori
Tomb, J.-F., White, O., Kerlavage, A. R., Clayton, R. A., Sutton, G. G., Fleischmann, R. D., Ketchum, K. A., Klenk, H. P., Gill, S., Dougherty, B. A., et al. (1997) · Nature · doi:10.1038/41483 · Cited by 3,529
Genes required for mycobacterial growth defined by high density mutagenesis
Sassetti, C. M., Boyd, D. H., & Rubin, E. J. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03425.x · Cited by 2,544
Alm, R. A., Ling, L.-S. L., Moir, D. T., King, B. L., Brown, E. D., Doig, P. C., Smith, D. R., Noonan, B., Guild, B. C., deJonge, B. L., et al. (1999) · Nature · doi:10.1038/16495 · Cited by 1,893
A minimal gene set for cellular life derived by comparison of complete bacterial genomes
Mushegian, A. R., & Koonin, E. V. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.19.10268 · Cited by 916
Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system
Yu, B. J., Sung, B. H., Koob, M. D., Lee, C. H., Lee, J. H., Lee, W. S., Kim, M. S., & Kim, S. C. (2002) · Nature Biotechnology · doi:10.1038/nbt740 · Cited by 145
Cited by
Essential genes of a minimal bacterium
Glass, J. I., Assad-Garcia, N., Alperovich, N., Yooseph, S., Lewis, M. R., Maruf, M., Hutchison, C. A., Smith, H. O., & Venter, J. C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0510013103 · Cited by 912
Hoy, B., Löwer, M., Weydig, C., Carra, G., Tegtmeyer, N., Geppert, T., Schröder, P., Sewald, N., Backert, S., Schneider, G., et al. (2010) · The EMBO Reports · doi:10.1038/embor.2010.114 · Cited by 295
Roberts, C. A., Al-Tameemi, H. M., Mashruwala, A. A., Rosario-Cruz, Z., Chauhan, U., Sause, W. E., Torres, V. J., Belden, W. J., & Boyd, J. M. (2017) · Infection and Immunity · doi:10.1128/iai.00100-17 · Cited by 84
Chang, Y.-C., Hu, Z., Rachlin, J., Anton, B. P., Kasif, S., Roberts, R. J., & Steffen, M. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkv1324 · Cited by 56
Systems biology perspectives on minimal and simpler cells
Xavier, J. C., Patil, K. R., & Rocha, I. (2014) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00050-13 · Cited by 58
Marques, M. S., Costa, A. C., Osório, H., Pinto, M. L., Relvas, S., Dinis-Ribeiro, M., Carneiro, F., Leite, M., & Figueiredo, C. (2021) · Gut Microbes · doi:10.1080/19490976.2021.1921928 · Cited by 23
Acharya, P., Lloyd, C., Lam, N. T., Kumke, J., Ray, S., Yanira Muñoz Ramirez, Z., Das, S., & Lam, H. N. (2026) · mSphere · doi:10.1128/msphere.00870-25 · Cited by 8
Metadata
- Authors
- Salama, N. R., Shepherd, B., & Falkow, S.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2004-12
- Volume
- 186
- Issue
- 23
- Pages
- 7926-7935
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2146245198
- Cited by
- 308
- References
- 5
- FWCI
- 16.66
- Type
- journal article
- Language
- en
- Open access
- green
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