Interaction of Candida albicans with Human Leukocytes and Serum
Open accessAbstract
A quantitative assay of candidacidal activity based on differential staining of non-viable Candida albicans by methylene blue was developed and applied to studies of leukocytes from normal individuals and patients with fungal and other infections. Serum factors were necessary for optimal phagocytosis of C. albicans but lacked direct candidacidal activity. Normal human neutrophils (38 studies) killed 29.0 ± 7.4% of ingested C. albicans in 1 hr. Eosinophils and monocytes killed a smaller percentage. Neutrophil candidacidal activity did not require protein or ribonucleic acid synthesis by the leukocyte but was inhibited by anaerobic conditions, potassium cyanide, and colchicine. Leukocytes of a patient with hereditary myeloperoxidase deficiency and of three children with chronic granulomatous disease phagocytized C. albicans normally, yet failed to kill them. Our data suggest that the neutrophil can play an important role in resistance to Candida infection and that the lysosomal enzyme myeloperoxidase and its oxidant substrate hydrogen peroxide are the major participants in neutrophil candidacidal activity.
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Neutrophils are essential for the host response to Candida.
“Neutrophils are essential for the host response to Candida (29, 30).”
From Dual function of <i>Candida auris</i> mannosyltransferase, MNT5, in biofilm community protection from antifungal therapy and the host (2025) · cites this paper as [30]
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- Authors
- Lehrer, R. I., & Cline, M. J.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1969-06
- Volume
- 98
- Issue
- 3
- Pages
- 996-1004
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC315286
- OpenAlex
- W2115568450
- Cited by
- 531
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 2 · 2018: 6 · 2017: 7 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 6 · 2012: 8
- FWCI
- 55.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Candida albicans, neutrophils, candidacidal activity, phagocytosis, myeloperoxidase, hydrogen peroxide, eosinophils, monocytes, chronic granulomatous disease, fungal infection resistance, serum factors, colchicine, potassium cyanide, anaerobic conditions
- MeSH terms
- Blood, Candida, Child, Chronic Disease, Eosinophils, Female, Granuloma, Humans, Immunity, Infections, Leukocytes, Male, Methods, Methylene Blue, Monocytes, Mycoses, Neutrophils, Peroxidases, Phagocytosis, Staining and Labeling
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