The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
High-Throughput Identification of Bacteria and Yeast by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Conventional Medical Microbiology Laboratories
Open accessAbstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is suitable for high-throughput and rapid diagnostics at low costs and can be considered an alternative for conventional biochemical and molecular identification systems in a conventional microbiological laboratory. First, we evaluated MALDI-TOF MS using 327 clinical isolates previously cultured from patient materials and identified by conventional techniques (Vitek-II, API, and biochemical tests). Discrepancies were analyzed by molecular analysis of the 16S genes. Of 327 isolates, 95.1% were identified correctly to genus level, and 85.6% were identified to species level by MALDI-TOF MS. Second, we performed a prospective validation study, including 980 clinical isolates of bacteria and yeasts. Overall performance of MALDI-TOF MS was significantly better than conventional biochemical systems for correct species identification (92.2% and 83.1%, respectively) and produced fewer incorrect genus identifications (0.1% and 1.6%, respectively). Correct species identification by MALDI-TOF MS was observed in 97.7% of Enterobacteriaceae , 92% of nonfermentative Gram-negative bacteria, 94.3% of staphylococci, 84.8% of streptococci, 84% of a miscellaneous group (mainly Haemophilus , Actinobacillus , Cardiobacterium , Eikenella , and Kingella [HACEK]), and 85.2% of yeasts. MALDI-TOF MS had significantly better performance than conventional methods for species identification of staphylococci and genus identification of bacteria belonging to HACEK group. Misidentifications by MALDI-TOF MS were clearly associated with an absence of sufficient spectra from suitable reference strains in the MALDI-TOF MS database. We conclude that MALDI-TOF MS can be implemented easily for routine identification of bacteria (except for pneumococci and viridans streptococci) and yeasts in a medical microbiological laboratory.
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“included clinical isolates and concluded that MALDI-TOF MS can replace conventional systems for identification of bacteria in a conventional laboratory (27).”
InfluentialPathogenesis of infections due to coagulasenegative staphylococci
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Cited by
Seng, P., Abat, C., Rolain, J. M., Colson, P., Lagier, J.-C., Gouriet, F., Fournier, P. E., Drancourt, M., La Scola, B., & Raoult, D. (2013) · Journal of Clinical Microbiology · doi:10.1128/jcm.00492-13 · Cited by 438
Lévesque, S., Dufresne, P. J., Soualhine, H., Domingo, M.-C., Bekal, S., Lefebvre, B., & Tremblay, C. (2015) · PLOS ONE · doi:10.1371/journal.pone.0144878 · Cited by 172
da Silva, T. F., Glória, R. D. A., Americo, M. F., Freitas, A. D. S., de Jesus, L. C. L., Barroso, F. A. L., Laguna, J. G., Coelho-Rocha, N. D., Tavares, L. M., le Loir, Y., et al. (2024) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-024-10247-x · Cited by 117
Mutters, N. T., Hodiamont, C. J., Jong, M. D. D., Overmeijer, H. P. J., Boogaard, M. V. D., & E.Visser, C. (2014) · Annals of Laboratory Medicine · doi:10.3343/alm.2014.34.2.111 · Cited by 82
Jensen, R. H., & Arendrup, M. C. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.02071-10 · Cited by 68
Metadata
- Authors
- van Veen, S. Q., Claas, E. C. J., & Kuijper, E. J.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-03
- Volume
- 48
- Issue
- 3
- Pages
- 900-907
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2145481069
- Cited by
- 667
- References
- 21
- FWCI
- 40.89
- Type
- journal article
- Language
- en
- Open access
- green
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