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Cited by 120Bacterial Infections and VaccinesBacterial Identification and Susceptibility TestingMicrobial infections and disease research

Detection of bacterial pathogens in Mongolia meningitis surveillance with a new real-time PCR assay to detect Haemophilus influenzae

Wang, Xin, Mair, Raydel, Hatcher, Cynthia, Theodore, M. Jordan, Edmond, Karen, Wu, Henry M., Harcourt, Brian H., Carvalho, Maria da Gloria S. +8 more·2011·International Journal of Medical Microbiology
DOI 10.1016/j.ijmm.2010.11.004
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Abstract

Since the implementation of Haemophilus influenzae (Hi) serotype b vaccine, other serotypes and non-typeable strains have taken on greater importance as a cause of Hi diseases. A rapid and accurate method is needed to detect all Hi regardless of the encapsulation status. We developed 2 real-time PCR (rt-PCR) assays to detect specific regions of the protein D gene (hpd). Both hpd assays are very specific and sensitive for detection of Hi. Of the 63 non-Hi isolates representing 21 bacterial species, none was detected by the hpd #1 assay, and only one of 2 H. aphrophilus isolates was detected by the hpd #3 assay. The hpd #1 and #3 assays detected 97% (229/237) and 99% (234/237) of Hi isolates, respectively, and were superior for detection of both typeable and non-typeable Hi isolates, as compared to previously developed rt-PCR targeting ompP2 or bexA. The diagnostic sensitivity and specificity of these rt-PCR assays were assessed on cerebrospinal fluid specimens collected as part of meningitis surveillance in Ulaanbaatar, Mongolia. The etiology (Neisseria meningitidis, Hi, and Streptococcus pneumoniae) of 111 suspected meningitis cases was determined by conventional methods (culture and latex agglutination), previously developed rt-PCR assays, and the new hpd assays. The rt-PCR assays were more sensitive for detection of meningitis pathogens than other classical methods and improved detection from 50% (56/111) to 75% (83/111). The hpd #3 assay identified a non-b Hi that was missed by the bexA assay and other methods. A sensitive rt-PCR assay to detect both typeable and non-typeable Hi is a useful tool for improving Hi disease surveillance especially after Hib vaccine introduction.

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  • The sensitivity of bexA PCR for Haemophilus influenzae ranged from 81% to 100% with a median of 93%, and specificity ranged from 81% to 100% with a median of 94%.

    “The sensitivity of bexA PCR ranged from 81% to 100% with a median of 93%, with a specificity ranging from 81% to 100% (median 94%) (54, 58, 65–68).”

    From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [67]

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Authors
Wang, X., Mair, R., Hatcher, C., Theodore, M. J., Edmond, K., Wu, H. M., Harcourt, B. H., Carvalho, M. D. G. S., Pimenta, F., Nymadawa, P., Altantsetseg, D., Kirsch, M., Satola, S. W., Cohn, A., Messonnier, N. E., & Mayer, L. W.
Affiliations
  • Centers for Disease Control and Prevention (US)
  • London School of Hygiene & Tropical Medicine (GB)
  • National Center for Communicable Diseases (MN)
  • Emory University (US)
Corresponding author
Wang, Xin
ORCID
Venue
International Journal of Medical Microbiology
Publisher
Elsevier BV
Published
2011-04
Volume
301
Issue
4
Pages
303-309
ISSN
1438-4221 (ISSN-L)
DOI
10.1016/j.ijmm.2010.11.004
OpenAlex
W2092170725
Cited by
120
References
39
Citations by year
2026: 2 · 2025: 2 · 2024: 8 · 2023: 2 · 2022: 6 · 2021: 14 · 2020: 9 · 2019: 9 · 2018: 5 · 2017: 9 · 2016: 8 · 2015: 8 · 2014: 8 · 2013: 10 · 2012: 14
FWCI
11.23
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • International Journal of Medical Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • UWA Profiles and Research Repository (UWA) (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
Haemophilus influenzae, Haemophilus influenzae type b, meningitis surveillance, real-time PCR, nontypeable Haemophilus influenzae, Aggregatibacter aphrophilus, Neisseria meningitidis, Streptococcus pneumoniae, cerebrospinal fluid, Haemophilus influenzae type b vaccine, sensitivity and specificity, Mongolia
MeSH terms
Bacteria, Bacteriological Techniques, Cerebrospinal Fluid, Child, Preschool, DNA, Bacterial, Haemophilus influenzae, Humans, Infant, Meningitis, Haemophilus, Molecular Sequence Data, Mongolia, Sensitivity and Specificity, Polymerase Chain Reaction, Sequence Analysis, DNA, DNA Primers

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