Rapid Identification of Candida Species with Species-Specific DNA Probes

Elie, Cheryl M.; Lott, Timothy J.; Reiss, Errol; Morrison, Christine J.

doi:10.1128/jcm.36.11.3260-3265.1998

Cited by 159Antifungal resistance and susceptibilityYeasts and Rust Fungi StudiesBacterial Identification and Susceptibility Testing

Rapid Identification of Candida Species with Species-Specific DNA Probes

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Elie, Cheryl M., Lott, Timothy J., Reiss, Errol, Morrison, Christine J.·1998·Journal of Clinical Microbiology

DOI 10.1128/jcm.36.11.3260-3265.1998PMC PMC105312

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Abstract

Rapid identification of Candida species has become more important because of an increase in infections caused by species other than Candida albicans , including species innately resistant to azole antifungal drugs. We previously developed a PCR assay with an enzyme immunoassay (EIA) format to detect amplicons from the five most common Candida species by using universal fungal primers and species-specific probes directed to the ITS2 region of the gene for rRNA. We designed probes to detect seven additional Candida species ( C. guilliermondii , C. kefyr , C. lambica , C. lusitaniae , C. pelliculosa , C. rugosa , and C. zeylanoides ) included in the API 20C sugar assimilation panel, five probes for species not identified by API 20C ( C. haemulonii , C. norvegica , C. norvegensis , C. utilis , and C. viswanathii ), and a probe for the newly described species C. dubliniensis , creating a panel of 18 Candida species probes. The PCR-EIA correctly identified multiple strains of each species tested, including five identified as C. albicans by the currently available API 20C database but determined to be C. dubliniensis by genotypic and nonroutine phenotypic characteristics. Species identification time was reduced from a mean of 3.5 days by conventional identification methods to 7 h by the PCR-EIA. This method is simple, rapid, and feasible for identifying Candida species in clinical laboratories that utilize molecular identification techniques and provides a novel method to differentiate the new species, C. dubliniensis , from C. albicans .

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When CDC and submitting laboratory identifications differed, isolate identification was confirmed by use of a DNA probe-based PCR-enzyme immunoassay method.
“When CDC and submitting laboratory identifications differed, isolate identification was confirmed by use of a DNA probe-based PCR-enzyme immunoassay method (7, 10).”
From Incidence of Bloodstream Infections Due to Candida Species and In Vitro Susceptibilities of Isolates Collected from 1998 to 2000 in a Population-Based Active Surveillance Program (2004) · cites this paper as [10]

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Rapid and unequivocal differentiation of Candida dubliniensis from other Candida species using species‐specific DNA probes: comparison with phenotypic identification methods
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Authors: Elie, C. M., Lott, T. J., Reiss, E., & Morrison, C. J.
Affiliations: Centers for Disease Control and Prevention (US)
Venue: Journal of Clinical Microbiology
Publisher: American Society for Microbiology
Published: 1998-11
Volume: 36
Issue: 11
Pages: 3260-3265
ISSN: 0095-1137 (ISSN-L)
1098-660X
DOI: 10.1128/jcm.36.11.3260-3265.1998
PMC: PMC105312
OpenAlex: W2152603420
Cited by: 159
References: 30
Citations by year: 2025: 2 · 2024: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2018: 1 · 2017: 6 · 2015: 4 · 2014: 6 · 2013: 2 · 2012: 2
FWCI: 6.03
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Journal of Clinical Microbiology (publishedVersion)
PubMed Central (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed (publishedVersion)
Funders: U.S. Department of Energy
Georgia State University
Centers for Disease Control and Prevention
Oak Ridge Institute for Science and Education
Topics: Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
Keywords: Candida species identification, species-specific DNA probes, Candida dubliniensis, Candida albicans, non-albicans Candida, azole resistance
MeSH terms: Base Sequence, Candida, Candida albicans, Candidiasis, Digoxigenin, Evaluation Studies as Topic, Genotype, Humans, Immunoenzyme Techniques, Mycology, Phenotype, Sensitivity and Specificity, Species Specificity, DNA Probes, Polymerase Chain Reaction

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Rapid Identification of Candida Species with Species-Specific DNA Probes

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