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Use of Multiplex PCR To Detect Classical and Newly Described Pyrogenic Toxin Genes in Staphylococcal Isolates
Open accessAbstract
Staphylococcus aureus may contain one or more genes that encode a variety of immunomodulatory pyrogenic toxins (PTs), including the staphylococcal enterotoxins and toxic shock syndrome toxin (TSST). The PTs interact with several cellular targets to produce disease, such as food poisoning and toxic shock syndrome. At present, nine serologically distinct enterotoxins and one immunoreactive form of TSST have been identified and characterized. As isolates of S. aureus are further assessed, it is anticipated that this number will increase. To facilitate screening, a multiplex PCR was designed to simultaneously determine which of these 10 currently known PT genes an individual S. aureus isolate possesses. We show here, using S. aureus isolates with characterized PT phenotypes, that this novel PCR technique reliably detects each of the known PTs in a single reaction.
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Cited by
Saiman, L., Keefe, M. O., Graham, P. L., Wu, F., Salim, B. S., Kreiswirth, B., LaSala, A., Schlievert, P. M., & Latta, P. D. (2003) · Clinical Infectious Diseases · doi:10.1086/379022 · Cited by 372
Coagulase-positive Staphylococci and Staphylococcus aureus in food products marketed in Italy
Normanno, G., Firinu, A., Virgilio, S., Mula, G., Dambrosio, A., Poggiu, A., Decastelli, L., Mioni, R., Scuota, S., Bolzoni, G., et al. (2005) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.05.008 · Cited by 274
Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates
Cassat, J. E., Dunman, P. M., McAleese, F., Murphy, E., Projan, S. J., & Smeltzer, M. S. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.2.576-592.2005 · Cited by 112
Metadata
- Authors
- Monday, S. R., & Bohach, G. A.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-10
- Volume
- 37
- Issue
- 10
- Pages
- 3411-3414
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2147086665
- Cited by
- 390
- References
- 0
- FWCI
- 3.73
- Type
- journal article
- Language
- en
- Open access
- green
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- Monday, S. R., & Bohach, G. A. (1999). Use of Multiplex PCR To Detect Classical and Newly Described Pyrogenic Toxin Genes in Staphylococcal Isolates. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.37.10.3411-3414.1999
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