Real-Time Fluorescence-Based PCR for Detection of Malaria Parasites
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A fluorescence-based real-time 5′ nuclease PCR capable of detecting all four human malaria parasites was developed to screen large numbers of samples during an outbreak to prevent further transmission of malaria. The effectiveness of antimalarial therapy for malaria patients can be monitored by determining the reduction of parasitemia by this method.
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In several studies using real-time PCR, either a single species was identified or no distinction between the four human parasites was made.
“In several studies in which real-time PCR was utilized, either a single-species was identified (13) or no distinction between the four human parasites was made (8, 18).”
From Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp (2005) · cites this paper as [18]
Real-time quantitative PCR methods have good sensitivity and range that allows monitoring parasitemia levels.
“Real-time quantitative PCR (qPCR) methods have followed, with good sensitivity and range that allows monitoring parasitemia levels [29,30].”
From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [29]
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Cites
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Self-citationDetection of Plasmodium falciparum by Polymerase Chain Reaction in a Field Study
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Perandin, F., Manca, N., Calderaro, A., Piccolo, G., Galati, L., Ricci, L., Medici, M. C., Arcangeletti, M. C., Snounou, G., Dettori, G., et al. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.3.1214-1219.2004 · Cited by 354 · see citing works
Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp
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Multiplex qPCR for Detection and Absolute Quantification of Malaria
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Diagnosing malaria in pregnancy: An update
Fried, M., Muehlenbachs, A., & Duffy, P. E. (2012) · Expert Review of Anti-infective Therapy · doi:10.1586/eri.12.98 · Cited by 80 · see citing works
- Authors
- Lee, M.-A., Tan, C.-H., Aw, L.-T., Tang, C.-S., Singh, M., Lee, S.-H., Chia, H.-P., & Yap, E. P. H.
- Affiliations
- Defence Science and Technology Agency (SG)
- Department of Microbiology
- National University Hospital (SG)
- National University of Singapore (SG)
- Defence Medical Research Institute, Defence Science and Technology Agency
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2002-11
- Volume
- 40
- Issue
- 11
- Pages
- 4343-4345
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC139664
- OpenAlex
- W2108065348
- Cited by
- 87
- References
- 10
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2021: 1 · 2019: 1 · 2018: 3 · 2017: 4 · 2016: 6 · 2015: 7 · 2014: 2 · 2013: 6 · 2012: 8
- FWCI
- 7.32
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Keywords
- malaria, Plasmodium, real-time PCR, 5' nuclease assay, fluorescence detection, malaria parasite detection, parasitemia
- MeSH terms
- Animals, Deoxyribonucleases, Humans, Malaria, Plasmodium, Sensitivity and Specificity, DNA, Protozoan, Polymerase Chain Reaction, Parasitemia, Taq Polymerase
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