The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay
Open accessAbstract
A rapid procedure was developed for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) directly from sterile sites or mixed flora samples (e.g., nose or inguinal swabs). After a rapid conditioning of samples, the method consists of two main steps: (i) immunomagnetic enrichment in S. aureus and (ii) amplification-detection profile on DNA extracts using multiplex quantitative PCR (5′-exonuclease qPCR, TaqMan). The triplex qPCR assay measures simultaneously the following targets: (i) mecA gene, conferring methicillin resistance, common to both S. aureus and Staphylococcus epidermidis ; (ii) femA gene from S. aureus ; and (iii) femA gene from S. epidermidis. This quantitative approach allows discrimination of the origin of the measured mecA signal. qPCR data were calibrated using two reference strains (MRSA and methicillin-resistant S. epidermidis ) processed in parallel to clinical samples. This 96-well format assay allowed analysis of 30 swab samples per run and detection of the presence of MRSA with exquisite sensitivity compared to optimal culture-based techniques. The complete protocol may provide results in less than 6 h (while standard procedure needs 2 to 3 days), thus allowing prompt and cost-effective implementation of contact precautions.
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
National Committee for Clinical Laboratory Standards
Bruck, E. (1980) · Pediatrics · doi:10.1542/peds.65.1.187 · Cited by 14,751
Mackay, I. M. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.6.1292 · Cited by 1,318
Simultaneous Amplification and Detection of Specific DNA Sequences
Higuchi, R., Dollinger, G., Walsh, P. S., & Griffith, R. (1992) · Bio/Technology · doi:10.1038/nbt0492-413 · Cited by 1,130
Linezolid resistance in a clinical isolate of Staphylococcus aureus
Tsiodras, S., Gold, H. S., Sakoulas, G., Eliopoulos, G. M., Wennersten, C., Venkataraman, L., Moellering, R. C., & Ferraro, M. J. (2001) · The Lancet · doi:10.1016/s0140-6736(01)05410-1 · Cited by 1,033
Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction
Murakami, K., Minamide, W., Wada, K., Nakamura, E., Teraoka, H., & Watanabe, S. (1991) · Journal of Clinical Microbiology · doi:10.1128/jcm.29.10.2240-2244.1991 · Cited by 942
Corless, C. E., Guiver, M., Borrow, R., Edwards-Jones, V., Fox, A. J., & Kaczmarski, E. B. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.4.1553-1558.2001 · Cited by 607
Chaix, C., Durand-Zaleski, I., Alberti, C., & Brun-Buisson, C. (1999) · JAMA · doi:10.1001/jama.282.18.1745 · Cited by 339
Abramson, M. A., & Sexton, D. J. (1999) · Infection Control & Hospital Epidemiology · doi:10.1086/501641 · Cited by 314
Pujol, M., Peña, C., Pallares, R., Ariza, J., Ayats, J., Dominguez, M. A., & Gudiol, F. (1996) · The American Journal of Medicine · doi:10.1016/s0002-9343(96)00014-9 · Cited by 289
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Strommenger, B., Kettlitz, C., Werner, G., & Witte, W. (2003) · Journal of Clinical Microbiology · doi:10.1128/jcm.41.9.4089-4094.2003 · Cited by 578
The Human Nasal Microbiota and Staphylococcus aureus Carriage
Frank, D. N., Feazel, L. M., Bessesen, M. T., Price, C. S., Janoff, E. N., & Pace, N. R. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010598 · Cited by 422
Huletsky, A., Giroux, R., Rossbach, V., Gagnon, M., Vaillancourt, M., Bernier, M., Gagnon, F., Truchon, K., Bastien, M., Picard, F. J., et al. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.5.1875-1884.2004 · Cited by 408
Mupirocin ointment for preventing Staphylococcus aureus infections in nasal carriers
van Rijen, M., Bonten, M., Wenzel, R., & Kluytmans, J. (2008) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd006216.pub2 · Cited by 286
Costa, A.-M., Kay, I., & Palladino, S. (2005) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2004.08.014 · Cited by 117
Metadata
- Authors
- Francois, P., Pittet, D., Bento, M., Pepey, B., Vaudaux, P., Lew, D., & Schrenzel, J.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2003-01
- Volume
- 41
- Issue
- 1
- Pages
- 254-260
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2113470060
- Cited by
- 294
- References
- 31
- FWCI
- 11.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Francois, P., Pittet, D., Bento, M., Pepey, B., Vaudaux, P., Lew, D., & Schrenzel, J. (2003). Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.41.1.254-260.2003
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