The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus
Open accessAbstract
In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus.Conditions were optimized to amplify fragments of mecA(encoding methicillin resistance),aacA-aphD(aminoglycoside resistance),tetK,tetM(tetracycline resistance),erm(A),erm(C) (macrolide-lincosamide-streptogramin B resistance),vat(A),vat(B), and vat(C) (streptogramin A resistance) simultaneously in one PCR amplification. An additional primer pair for the amplification of a fragment of the staphylococcal 16S rDNA was included as a positive control. The multiplex PCR assay was evaluated on 30 different S. aureus isolates, and the PCR results correlated with the phenotypic antibiotic resistance data obtained by the broth microdilution assay. The multiplex PCR assay offers a rapid, simple, and accurate identification of antibiotic resistance profiles and could be used in clinical diagnosis as well as for the surveillance of the spread of antibiotic resistance determinants in epidemiological studies.
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Cites
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Tsiodras, S., Gold, H. S., Sakoulas, G., Eliopoulos, G. M., Wennersten, C., Venkataraman, L., Moellering, R. C., & Ferraro, M. J. (2001) · The Lancet · doi:10.1016/s0140-6736(01)05410-1 · Cited by 1,033
Multiplex PCR: Critical Parameters and Step-by-Step Protocol
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Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus
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Cited by
Efflux-mediated antimicrobial resistance
Poole, K. (2005) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dki171 · Cited by 1,082
Antibiotic resistance in non-enterococcal lactic acid bacteria and bifidobacteria
Ammor, M. S., Belén Flórez, A., & Mayo, B. (2007) · Food Microbiology · doi:10.1016/j.fm.2006.11.001 · Cited by 460
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Metadata
- Authors
- Strommenger, B., Kettlitz, C., Werner, G., & Witte, W.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2003-09
- Volume
- 41
- Issue
- 9
- Pages
- 4089-4094
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2158830887
- Cited by
- 578
- References
- 30
- FWCI
- 6.32
- Type
- journal article
- Language
- en
- Open access
- green
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- Strommenger, B., Kettlitz, C., Werner, G., & Witte, W. (2003). Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.41.9.4089-4094.2003
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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