The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Development of a Multiplex PCR for the Detection of asa1 , gelE , cylA , esp , and hyl Genes in Enterococci and Survey for Virulence Determinants among European Hospital Isolates of Enterococcus faecium
Open accessAbstract
A multiplex PCR for the simultaneous detection of five virulence genes ( asa1 , gelE , cylA , esp , and hyl ) in enterococci was developed. The presence of these genes was investigated in 153 clinical and 118 fecal Enterococcus faecium isolates from inpatients at an increased risk of developing infections (such as patients in intensive care units and hematology wards) from 13 hospitals in eight European countries. Of the 271 E. faecium isolates, 135 were vancomycin resistant E. faecium (VREF) isolates and 136 were vancomycin susceptible E. faecium (VSEF) isolates. Susceptibilities to ampicillin, gentamicin, streptomycin, vancomycin, teicoplanin, ramoplanin, quinupristin-dalfopristin, and linezolid were tested by the microdilution method. Overall, the prevalence of esp was significantly higher ( P = 0.03) in clinical VREF isolates (92%) than in fecal VREF isolates (73%). In Italy, the prevalence of esp was significantly higher ( P = 0.02) in VREF isolates (91%) than in VSEF isolates (68%), whereas in the United Kingdom, hyl was significantly more prevalent ( P = 0.01) in VREF isolates (71%) than in VSEF isolates (29%). No significant differences were found for the other countries. Pulsed-field gel electrophoresis was used to check the clonality among the strains tested and showed the spread of two center-specific ( esp -positive) VREF clones in Italy and one center-specific ( hyl -positive) clone in the United Kingdom. These clones were resistant to ampicillin, gentamicin, and streptomycin. The multiplex PCR reported in this study is a convenient and rapid method for the simultaneous detection of the virulence genes asa1 , gelE , cylA , esp , and hyl in enterococci. Molecular analysis showed the intrahospital spread of esp -positive VREF clones (in Italy) and hyl -positive VREF clones (in the United Kingdom); the role of hyl remains to be elucidated.
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Domingos-Lopes, M. F. P., Stanton, C., Ross, P. R., Dapkevicius, M. L. E., & Silva, C. C. G. (2017) · Food Microbiology · doi:10.1016/j.fm.2016.11.014 · Cited by 210
Li, M., Wang, Y., Cui, H., Li, Y., Sun, Y., & Qiu, H.-J. (2020) · Frontiers in Veterinary Science · doi:10.3389/fvets.2020.00049 · Cited by 179
de Souza, B. M. S., Borgonovi, T. F., Casarotti, S. N., Todorov, S. D., & Penna, A. L. B. (2019) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-018-9406-y · Cited by 177
Singhal, N., Maurya, A. K., Mohanty, S., Kumar, M., & Virdi, J. S. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01567 · Cited by 94
Ghosh, A., Borst, L., Stauffer, S. H., Suyemoto, M., Moisan, P., Zurek, L., & Gookin, J. L. (2013) · Journal of Clinical Microbiology · doi:10.1128/jcm.00481-13 · Cited by 44
Metadata
- Authors
- Vankerckhoven, V., Van Autgaerden, T., Vael, C., Lammens, C., Chapelle, S., Rossi, R., Jabes, D., & Goossens, H.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-10
- Volume
- 42
- Issue
- 10
- Pages
- 4473-4479
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2148615734
- Cited by
- 594
- References
- 38
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- green
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- Vankerckhoven, V., Van Autgaerden, T., Vael, C., Lammens, C., Chapelle, S., Rossi, R., Jabes, D., & Goossens, H. (2004). Development of a Multiplex PCR for the Detection of asa1 , gelE , cylA , esp , and hyl Genes in Enterococci and Survey for Virulence Determinants among European Hospital Isolates of Enterococcus faecium. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.42.10.4473-4479.2004
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