Cited by 131Antibiotic Resistance in BacteriaDrug Transport and Resistance MechanismsBacterial Genetics and Biotechnology

Detection and Prevalence of Active Drug Efflux Mechanism in Various Multidrug-Resistant Klebsiella pneumoniae Strains from Turkey

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Hasdemir, Ufuk Over, Chevalier, Jacqueline, Nordmann, Patrice, Pagès, Jean-Marie·2004·Journal of Clinical Microbiology

Abstract

The prevalence of active drug efflux pump and porin alterations was investigated in Turkish nosocomial strains of Klebsiella pneumoniae exhibiting a multidrug-resistant phenotype. MICs of various antibiotics, including quinolones, chloramphenicol, tetracycline, and β-lactams, for those strains were determined either with or without the efflux pump inhibitor phenylalanine arginine β-naphthylamide (PAβN). Thirty-nine percent of the strains exhibited a PAβN-modulated resistance for quinolones, chloramphenicol, and tetracycline. In these strains, a significant increase of chloramphenicol accumulation was gained in the presence of the efflux pump inhibitor PAβN or with the energy uncoupler carbonyl cyanide m -chlorophenylhydrazone. Moreover, high-level expression of the membrane fusion protein AcrA, which was immunodetected in most of those isolates, suggests that the AcrAB/TolC efflux machinery contributed to their antibiotic resistance. Studies of K. pneumoniae porins indicated that the majority of the strains, including extended-spectrum β-lactamase producers and efflux-positive ones, presented an alteration in their sorbitol-sensitive porin (OmpK35) expression. This is the first report showing the prominent role of active drug efflux in the antibiotic resistance of nosocomial K. pneumoniae strains from Turkey.

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Crossref · 10.1128/jcm.42.6.2701-2706.2004

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  • This porin shift correlated with a 4-8 fold decrease in susceptibility for a wide range of beta-lactam antimicrobials.

    “This alteration in the type of porin expressed correlated with a 4 – 8 fold decrease in susceptibility for a wide range of β-lactam antimicrobials (41,42).”

    From Mechanisms of Antibiotic Resistance (2016) · cites this paper as [41]

  • K. pneumoniae post-therapy isolates exhibit a shift in porin expression from OmpK35 to OmpK36, the latter having a smaller channel size.

    “The post-therapy isolates were found to exhibit a shift in porin expression from OmpK35 to OmpK36 (the latter possessing a smaller channel size).”

    From Mechanisms of Antibiotic Resistance (2016) · cites this paper as [41]

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Authors
Hasdemir, U. O., Chevalier, J., Nordmann, P., & Pagès, J.-M.
Affiliations
  • Aix-Marseille Université (FR)
  • Bicêtre Hospital (FR)
  • Enveloppe Bactérienne, Perméabilité et Antibiotiques, EA 2197, IFR48, Faculté de Médecine, Université de la Mediterranée, Marseille
Venue
Journal of Clinical Microbiology
Published
2004-06
Volume
42
Issue
6
Pages
2701-2706
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W2151288894
Cited by
131
References
30
Citations by year
2026: 1 · 2025: 4 · 2024: 5 · 2023: 7 · 2022: 3 · 2021: 7 · 2020: 8 · 2019: 6 · 2018: 7 · 2017: 7 · 2016: 16 · 2015: 2 · 2014: 9 · 2013: 7 · 2012: 2
FWCI
2.58
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Dspace Repository (Marmara Üniversitesi) (submittedVersion, other-oa)
Funders
  • AstraZeneca
  • European Society of Clinical Microbiology and Infectious Diseases
  • Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Klebsiella pneumoniae, multidrug resistance, AcrAB-TolC efflux system, AcrA, OmpK35, PAβN, CCCP, quinolone resistance, chloramphenicol resistance, tetracycline resistance, beta-lactam resistance, hospital isolates, extended-spectrum beta-lactamase, Turkey
MeSH terms
Bacterial Outer Membrane Proteins, Bacterial Proteins, Carbonyl Cyanide m-Chlorophenyl Hydrazone, Chloramphenicol, Hydrolases, Klebsiella pneumoniae, Microbial Sensitivity Tests, Porins, Drug Resistance, Multiple, Bacterial, Escherichia coli Proteins

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