The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Molecular Analysis of the Microflora Associated with Dental Caries
Open accessAbstract
Molecular techniques have revealed many novel, presumed unculturable, taxa in oral infections. The aim of this study was to characterize the bacterial community of the middle and advancing front of carious dental lesions by cultural and molecular analyses. Samples were collected with a hand excavator from five teeth with carious lesions involving dentine. Samples were cultured on blood agar and Rogosa agar incubated in air plus 5% CO 2 and on fastidious anaerobe agar anaerobically. DNA was also extracted directly from the samples and 16S rRNA genes were amplified by PCR with universal primers. PCR products were singularized by cloning, and the cloned inserts and cultured isolates were identified by 16S rRNA gene sequence analysis. We identified 95 taxa among the 496 isolates and 1,577 clones sequenced; 44 taxa were detected by the molecular method alone; 31 taxa were previously undescribed. Only three taxa, Streptococcus mutans , Rothia dentocariosa , and an unnamed Propionibacterium sp., were found in all five samples. The predominant taxa by anaerobic cultivation were the novel Propionibacterium sp. (18%), Olsenella profusa (14%), and Lactobacillus rhamnosus (8%). The predominant taxa in the molecular analysis were Streptococcus mutans (16%), Lactobacillus gasseri / johnsonii (13%), and Lactobacillus rhamnosus (8%). There was no significant difference between the compositions of the microflora in the middle and advancing front samples ( P < 0.05, Wilcoxon matched pairs, signed ranks test). In conclusion, combined cultural and molecular analyses have shown that a diverse bacterial community is found in dentinal caries and that numerous novel taxa are present.
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Thompson, J. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.24.4876 · Cited by 38,993
PHYLIP-Phylogeny inference package (Version 3.2)
Felsenstein, J. (1989) · Cladistics · Cited by 17,682
Tree View: An application to display phylogenetic trees on personal computers
Page, R. D. M. (1996) · Bioinformatics · doi:10.1093/bioinformatics/12.4.357 · Cited by 10,178
Nucleic acid techniques in bacterial systematics
Stackebrandt, E. S., & Goodfellow, M. (1991) · Wiley eBooks · Cited by 9,460
Lane, D. (1991) · Medical Entomology and Zoology · Cited by 8,187
Occlusal Caries: Biological Approach for Its Diagnosis and Management
Carvalho, J. C., Dige, I., Machiulskiene, V., Qvist, V., Bakhshandeh, A., Fatturi-Parolo, C., & Maltz, M. (2016) · Caries Research · doi:10.1159/000448662 · Cited by 2,153
Bacterial Diversity in Human Subgingival Plaque
Paster, B. J., Boches, S. K., Galvin, J. L., Ericson, R. E., Lau, C. N., Levanos, V. A., Sahasrabudhe, A., & Dewhirst, F. E. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.12.3770-3783.2001 · Cited by 2,011
Bias in template-to-product ratios in multitemplate PCR
Polz, M. F., & Cavanaugh, C. M. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.10.3724-3730.1998 · Cited by 1,484
Ecological Hypothesis of Dentin and Root Caries
Takahashi, N., & Nyvad, B. (2016) · Caries Research · doi:10.1159/000447309 · Cited by 733
Molecular Analysis of Bacterial Species Associated with Childhood Caries
Becker, M. R., Paster, B. J., Leys, E. J., Moeschberger, M. L., Kenyon, S. G., Galvin, J. L., Boches, S. K., Dewhirst, F. E., & Griffen, A. L. (2002) · Journal of Clinical Microbiology · doi:10.1128/jcm.40.3.1001-1009.2002 · Cited by 546
Cited by
Dewhirst, F. E., Chen, T., Izard, J., Paster, B. J., Tanner, A. C. R., Yu, W.-H., Lakshmanan, A., & Wade, W. G. (2010) · Journal of Bacteriology · doi:10.1128/jb.00542-10 · Cited by 3,352
Self-citationThe oral microbiome in health and disease
Wade, W. G. (2013) · Pharmacological Research · doi:10.1016/j.phrs.2012.11.006 · Cited by 1,347
Self-citationMolecular analysis of the bacterial microbiota in the human stomach
Bik, E. M., Eckburg, P. B., Gill, S. R., Nelson, K. E., Purdom, E. A., Francois, F., Perez-Perez, G., Blaser, M. J., & Relman, D. A. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506655103 · Cited by 1,158
Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults
Aas, J. A., Griffen, A. L., Dardis, S. R., Lee, A. M., Olsen, I., Dewhirst, F. E., Leys, E. J., & Paster, B. J. (2008) · Journal of Clinical Microbiology · doi:10.1128/jcm.01410-07 · Cited by 952
Managing Carious Lesions: Consensus Recommendations on Carious Tissue Removal
Schwendicke, F., Frencken, J. E., Bjørndal, L., Maltz, M., Manton, D. J., Ricketts, D., Van Landuyt, K., Banerjee, A., Campus, G., Doméjean, S., et al. (2016) · Advances in Dental Research · doi:10.1177/0022034516639271 · Cited by 731
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Metadata
- Authors
- Munson, M. A., Banerjee, A., Watson, T. F., & Wade, W. G.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-07
- Volume
- 42
- Issue
- 7
- Pages
- 3023-3029
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2158157679
- Cited by
- 401
- References
- 27
- FWCI
- 18.89
- Type
- journal article
- Language
- en
- Open access
- green
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