The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Detection and Typing of Human Papillomavirus by E6 Nested Multiplex PCR
Open accessAbstract
A nested multiplex PCR (NMPCR) assay that combines degenerate E6/E7 consensus primers and type-specific primers was evaluated for the detection and typing of human papillomavirus (HPV) genotypes 6/11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 51, 52, 56, 58, 59, 66, and 68 using HPV DNA-containing plasmids and cervical scrapes ( n = 1,525). The performance of the NMPCR assay relative to that of conventional PCR with MY09-MY11 and GP5+-GP6+ primers, and nested PCR with these two primer sets (MY/GP) was evaluated in 495 cervical scrapes with corresponding histologic and cytologic findings. HPV prevalence rates determined with the NMPCR assay were 34.7% (102 of 294) in the absence of cervical intraepithelial neoplasia (CIN 0), 94.2% (113 of 120) in the presence of mild or moderate dysplasia (CIN I/II), and 97.8% (44 of 45) in the presence of severe dysplasia (CIN III). The combination of all four HPV detection methods applied in the study was taken as “gold standard”: in all three morphological subgroups the NMPCR assay had significantly ( P < 0.0001) higher sensitivities than the MY09-MY11 and GP5+-GP6+ assays and sensitivities comparable or equal to those of the MY/GP assay. All 18 HPV genotypes investigated were detected among the clinical samples. The ratio of high- to low-risk HPV genotypes increased from 4:1 (80 of 103) in CIN 0 to 19:1 (149 of 157) in CIN I to III. Multiple infections were detected in 47.9% (124 of 259) of the patients. In conclusion, the novel NMPCR method is a sensitive and useful tool for HPV DNA detection, especially when exact HPV genotyping and the identification of multiple HPV infections are required.
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Cites
Prevalence of Human Papillomavirus in Cervical Cancer: a Worldwide Perspective
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Cited by
Seiwert, T. Y., Zuo, Z., Keck, M. K., Khattri, A., Pedamallu, C. S., Stricker, T., Brown, C., Pugh, T. J., Stojanov, P., Cho, J., et al. (2015) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-13-3310 · Cited by 657
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Global Proficiency Study of Human Papillomavirus Genotyping
Eklund, C., Zhou, T., & Dillner, J. (2010) · Journal of Clinical Microbiology · doi:10.1128/jcm.00918-10 · Cited by 122
Senapati, R., Nayak, B., Kar, S. K., & Dwibedi, B. (2017) · BMC Infectious Diseases · doi:10.1186/s12879-016-2136-4 · Cited by 95
“Primers were obtained by following the available literature [12].”
MethodKrupar, R., Robold, K., Gaag, D., Spanier, G., Kreutz, M., Renner, K., Hellerbrand, C., Hofstaedter, F., & Bosserhoff, A. K. (2014) · Virchows Archiv · doi:10.1007/s00428-014-1630-6 · Cited by 75
Metadata
- Authors
- Sotlar, K., Diemer, D., Dethleffs, A., Hack, Y., Stubner, A., Vollmer, N., Menton, S., Menton, M., Dietz, K., Wallwiener, D., Kandolf, R., & Bültmann, B.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-07
- Volume
- 42
- Issue
- 7
- Pages
- 3176-3184
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1968986340
- Cited by
- 321
- References
- 29
- FWCI
- 3.38
- Type
- journal article
- Language
- en
- Open access
- green
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- Sotlar, K., Diemer, D., Dethleffs, A., Hack, Y., Stubner, A., Vollmer, N., Menton, S., Menton, M., Dietz, K., Wallwiener, D., Kandolf, R., & Bültmann, B. (2004). Detection and Typing of Human Papillomavirus by E6 Nested Multiplex PCR. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.42.7.3176-3184.2004
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