The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Influenza A Virus PB1-F2 Protein Contributes to Viral Pathogenesis in Mice
Abstract
The influenza virus PB1-F2 protein is a novel protein previously shown to be involved in induction of cell death. Here we characterize the expression and the function of the protein within the context of influenza viral infection in tissue culture and a mouse model. We show that the C-terminal region of the protein can be expressed from a downstream initiation codon and is capable of interaction with the full-length protein. Using this knowledge, we generated influenza viruses knocked out for the expression of PB1-F2 protein and its downstream truncation products. Knocking out the PB1-F2 protein had no effect on viral replication in tissue culture but diminished virus pathogenicity and mortality in mice. The viruses replicated to similar levels in mouse lungs by day 3 postinfection, suggesting that the knockout did not impair viral replication. However, while the PB1-F2 knockout viruses were cleared after day 5, the wild-type viruses were detectable in mouse lungs until day 7, implying that expression of PB1-F2 resulted in delayed clearance of the viruses by the host immune system. Based on our findings and on the fact that the PB1 genomic segment was always newly introduced into some pandemic influenza viruses of the last century, we speculate that the PB1-F2 protein plays an important role in pathogenesis of influenza virus infection and may be an important contributor to pathogenicity of pandemic influenza viruses.
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Self-citationA novel influenza A virus mitochondrial protein that induces cell death
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Self-citationAvian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics
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Influenza Virus PB1-F2 Protein Induces Cell Death through Mitochondrial ANT3 and VDAC1
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Matrix Protein 2 of Influenza A Virus Blocks Autophagosome Fusion with Lysosomes
Gannagé, M., Dormann, D., Albrecht, R., Dengjel, J., Torossi, T., Rämer, P. C., Lee, M., Strowig, T., Arrey, F., Conenello, G., et al. (2009) · Cell Host & Microbe · doi:10.1016/j.chom.2009.09.005 · Cited by 526
McAuley, J. L., Hornung, F., Boyd, K. L., Smith, A. M., McKeon, R., Bennink, J., Yewdell, J. W., & McCullers, J. A. (2007) · Cell Host & Microbe · doi:10.1016/j.chom.2007.09.001 · Cited by 389
McAuley, J. L., Zhang, K., & McCullers, J. A. (2010) · Journal of Virology · doi:10.1128/jvi.01785-09 · Cited by 110
Metadata
- Authors
- Zamarin, D., Ortigoza, M. B., & Palese, P.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2006-08-15
- Volume
- 80
- Issue
- 16
- Pages
- 7976-7983
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2133318493
- Cited by
- 304
- References
- 25
- FWCI
- 14.17
- Type
- journal article
- Language
- en
- Open access
- closed
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