Characterization of the reconstructed 1918 Spanish influenza pandemic virus
Abstract
The pandemic influenza virus of 1918–1919 killed an estimated 20 to 50 million people worldwide. With the recent availability of the complete 1918 influenza virus coding sequence, we used reverse genetics to generate an influenza virus bearing all eight gene segments of the pandemic virus to study the properties associated with its extraordinary virulence. In stark contrast to contemporary human influenza H1N1 viruses, the 1918 pandemic virus had the ability to replicate in the absence of trypsin, caused death in mice and embryonated chicken eggs, and displayed a high-growth phenotype in human bronchial epithelial cells. Moreover, the coordinated expression of the 1918 virus genes most certainly confers the unique high-virulence phenotype observed with this pandemic virus.
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From Human innovation and the creative agency of the world in the age of generative AI (2024) · cites this paper as [14]
Descendants of 1918 influenza virus still circulating confer cross-reactive immunity to their ancestor, which shares the same H1N1 antigen profile.
“Descendants of 1918 influenza virus still circulating in populations today confer cross-reactive immunity to their ancestor, which shares the same H1N1 antigen profile 14 .”
From Human innovation and the creative agency of the world in the age of generative AI (2024) · cites this paper as [14]
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- Authors
- Tumpey, T. M., Basler, C. F., Aguilar, P. V., Zeng, H., Solórzano, A., Swayne, D. E., Cox, N. J., Katz, J. M., Taubenberger, J. K., Palese, P., & García-Sastre, A.
- Affiliations
- Centers for Disease Control and Prevention (US)
- Influenza Branch, Mailstop G-16, Division of Viral and Rickettsial Diseases (DVRD), National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, NE, Atlanta, GA 30333, USA.
- Corresponding author
- Tumpey, Terrence M.
- ORCID
- Venue
- Science
- Published
- 2005-10-07
- Volume
- 310
- Issue
- 5745
- Pages
- 77-80
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2104148197
- Cited by
- 1,327
- References
- 0
- Citations by year
- 2026: 12 · 2025: 54 · 2024: 29 · 2023: 32 · 2022: 79 · 2021: 56 · 2020: 50 · 2019: 53 · 2018: 39 · 2017: 33 · 2016: 44 · 2015: 35 · 2014: 75 · 2013: 60 · 2012: 65
- FWCI
- 73.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- DigitalGeorgetown (Georgetown University Library) (submittedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (U19 AI62623, U54 AI 57158, U19AI062623, P01AI058113, U54AI057158)
- Topics
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Keywords
- reverse genetics, viral virulence, mouse lethality, embryonated chicken eggs, human bronchial epithelial cells, H1N1 influenza, viral gene expression
- MeSH terms
- Animals, Bronchi, Cell Line, Chick Embryo, Female, Genes, Viral, Genetic Techniques, Humans, Influenza, Human, Lung, Mice, Inbred BALB C, Neuraminidase, Orthomyxoviridae Infections, Viral Plaque Assay, Recombination, Genetic, RNA, Viral, Trypsin, Virulence, Virus Replication, Hemagglutinin Glycoproteins, Influenza Virus, Respiratory Mucosa, History, 20th Century, Mice, Influenza A Virus, H1N1 Subtype
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