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Rescue of Influenza A Virus from Recombinant DNA
Open accessAbstract
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.
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Cited by
Characterization of the reconstructed 1918 Spanish influenza pandemic virus
Tumpey, T. M., Basler, C. F., Aguilar, P. V., Zeng, H., Solórzano, A., Swayne, D. E., Cox, N. J., Katz, J. M., Taubenberger, J. K., Palese, P., et al. (2005) · Science · doi:10.1126/science.1119392 · Cited by 1,327
Self-citationThe Pathology of Influenza Virus Infections
Taubenberger, J. K., & Morens, D. M. (2008) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev.pathmechdis.3.121806.154316 · Cited by 1,111
RIG-I detects viral genomic RNA during negative-strand RNA virus infection
Rehwinkel, J., Tan, C. P., Goubau, D., Schulz, O., Pichlmair, A., Bier, K., Robb, N., Vreede, F., Barclay, W., Fodor, E., et al. (2010) · Cell · doi:10.1016/j.cell.2010.01.020 · Cited by 585
Self-citationGenomic analysis of increased host immune and cell death responses induced by 1918 influenza virus
Kash, J. C., Tumpey, T. M., Proll, S. C., Carter, V., Perwitasari, O., Thomas, M. J., Basler, C. F., Palese, P., Taubenberger, J. K., García-Sastre, A., et al. (2006) · Nature · doi:10.1038/nature05181 · Cited by 551
Self-citationMatrix Protein 2 of Influenza A Virus Blocks Autophagosome Fusion with Lysosomes
Gannagé, M., Dormann, D., Albrecht, R., Dengjel, J., Torossi, T., Rämer, P. C., Lee, M., Strowig, T., Arrey, F., Conenello, G., et al. (2009) · Cell Host & Microbe · doi:10.1016/j.chom.2009.09.005 · Cited by 526
Metadata
- Authors
- Fodor, E., Devenish, L., Engelhardt, O. G., Palese, P., Brownlee, G. G., & García-Sastre, A.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1999-11
- Volume
- 73
- Issue
- 11
- Pages
- 9679-9682
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2146389912
- Cited by
- 775
- References
- 0
- FWCI
- 21.18
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Rescue of Influenza A Virus from Recombinant DNA. https://app.cochat.ai/papers/10.1128/jvi.73.11.9679-9682.1999
- The paper
- Fodor, E., Devenish, L., Engelhardt, O. G., Palese, P., Brownlee, G. G., & García-Sastre, A. (1999). Rescue of Influenza A Virus from Recombinant DNA. Journal of Virology. https://doi.org/10.1128/jvi.73.11.9679-9682.1999
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
