The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Regulation of cytomegalovirus gene expression: alpha and beta promoters are trans activated by viral functions in permissive human fibroblasts
Open accessAbstract
We have fused immediate (alpha) and delayed (beta) early promoter-regulatory sequences taken from the cytomegalovirus (CMV) genome to Escherichia coli lacZ (beta-galactosidase) as an indicator gene to study regulated expression of these promoters. After transfection of human fibroblast cells with plasmid constructs carrying beta-galactosidase fusions, and subsequent infection with CMV, we have demonstrated that viral trans-acting functions up-regulate the expression of these genes in a temporally authentic manner. The alpha promoter is activated even when de novo protein synthesis is blocked and when UV-inactivated virus is used, suggesting that, as for herpes simplex virus type 1 (HSV-1), a virion structural protein is responsible for its up-regulation. We have found that HSV-1, as well as CMV, is capable of trans activating the CMV alpha promoter. The beta promoter is activated by CMV but is completely unresponsive to HSV-1 infection. The temporal synthesis of the alpha and beta promoters in the transient expression system conforms with their natural regulation during viral replication. The beta-galactosidase fusions we describe provide a most exquisitely sensitive indicator system for the study of cis- and trans-acting viral regulatory functions.
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Citations
Cites
Expression of a β-globin gene is enhanced by remote SV40 DNA sequences
Banerji, J., Rusconi, S., & Schaffner, W. (1981) · Cell · doi:10.1016/0092-8674(81)90413-x · Cited by 1,724
In vivo sequence requirements of the SV40 early promoter region
Benoist, C., & Chambon, P. (1981) · Nature · doi:10.1038/290304a0 · Cited by 1,074
Mocarski, E. S., Post, L. E., & Roizman, B. (1980) · Cell · doi:10.1016/0092-8674(80)90172-5 · Cited by 186
Self-citation
Cited by
Analysis of the Protein-Coding Content of the Sequence of Human Cytomegalovirus Strain AD169
Chee, M. S., Bankier, A. T., Beck, S., Bohni, R., Brown, C. M., Cerny, R., Horsnell, T., Hutchison, C. A., Kouzarides, T., Martignetti, J. A., et al. (1990) · Current Topics in Microbiology and Immunology · doi:10.1007/978-3-642-74980-3_6 · Cited by 1,252
Multiple promoters direct tissue-specific expression of the rat BDNF gene
Timmusk, T., Palm, K., Metsis, M., Reintam, T., Paalme, V., Saarma, M., & Persson, H. (1993) · Neuron · doi:10.1016/0896-6273(93)90335-o · Cited by 858
Nissen, R. M., & Yamamoto, K. R. (2000) · Genes & Development · doi:10.1101/gad.827900 · Cited by 559
Cherrington, J. M., & Mocarski, E. S. (1989) · Journal of Virology · doi:10.1128/jvi.63.3.1435-1440.1989 · Cited by 307
Self-citationSmith, I. L., Hardwicke, M. A., & Sandri-Goldin, R. M. (1992) · Virology · doi:10.1016/0042-6822(92)90062-t · Cited by 278
Metadata
- Authors
- Spaete, R. R., & Mocarski, E. S.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1985-10
- Volume
- 56
- Issue
- 1
- Pages
- 135-143
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2101290459
- Cited by
- 217
- References
- 3
- FWCI
- 9.33
- Type
- journal article
- Language
- en
- Open access
- green
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- Spaete, R. R., & Mocarski, E. S. (1985). Regulation of cytomegalovirus gene expression: alpha and beta promoters are trans activated by viral functions in permissive human fibroblasts. Journal of Virology. https://doi.org/10.1128/jvi.56.1.135-143.1985
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