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The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes
Abstract
The early human papillomavirus type 16 genes that directly participate in the in vitro transformation of primary human keratinocytes have been defined. In the context of the full viral genome, mutations in either the E6 or E7 open reading frame completely abrogated transformation of these cells. Mutations in the E1, E2, and E2-E4 open reading frames, on the other hand, had no effect. Thus, both the full-length E6 and E7 genes were required for the induction of keratinocyte immortalization and resistance to terminal differentiation. The E6 and E7 genes expressed together from the human beta-actin promoter were sufficient for this transformation; mutation of either gene in the context of this recombinant plasmid eliminated the ability to induce stable differentiation-resistant transformants.
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“HPV-16 E6/E7-expressing cell lines were immortalized but were not tumourigenic in nude mice, although they could induce tumours after several passages or in cooperation with additional oncogenes such as ras or fos [79,90,91,92]”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [90]
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“both E6 and E7 cooperate in transformation, where E7 drives the early tumourigenesis and E6 modulates the progression towards malignancy [90,98]”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [90]
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Citations
Cites
The human papilloma virus-16 E7-oncoprotein is able to bind to the retinoblastoma gene product
Dyson, N., Howley, P. M., Münger, K., & Harlow, E. (1989) · Science · doi:10.1126/science.2537532 · Cited by 3,017
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Cited by
The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53
Scheffner, M., Werness, B. A., Huibregtse, J. M., Levine, A. J., & Howley, P. M. (1990) · Cell · doi:10.1016/0092-8674(90)90409-8 · Cited by 3,995
Self-citationAssociation of Human Papillomavirus Types 16 and 18 E6 Proteins with p53
Werness, B. A., Levine, A. J., & Howley, P. M. (1990) · Science · doi:10.1126/science.2157286 · Cited by 2,562
Self-citationA role for sunlight in skin-cancer – UV-induced p53 mutations in squamous-cell carcinoma
Brash, D. E., Rudolph, J. A., Simon, J. A., Lin, A., McKenna, G. J., Baden, H. P., Halperin, A. J., & Pontén, J. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.22.10124 · Cited by 1,959
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Metadata
- Authors
- Münger, K., Phelps, W. C., Bubb, V, Howley, P. M., & Schlegel, R.
- Affiliations
- Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1989-10
- Volume
- 63
- Issue
- 10
- Pages
- 4417-4421
- ISSN
- 0022-538X
- 1098-5514
- Cited by
- 1,030
- References
- 1
- Language
- en
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- CoChat. (2026). Citation check: The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. https://app.cochat.ai/papers/10.1128/jvi.63.10.4417-4421.1989
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- Münger, K., Phelps, W. C., Bubb, V, Howley, P. M., & Schlegel, R. (1989). The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Journal of Virology. https://doi.org/10.1128/jvi.63.10.4417-4421.1989
