The DOI matches its Crossref record; 38 of the 39 references it cites were found in a registry or an index.
Construction of less neurovirulent polioviruses by introducing deletions into the 5' noncoding sequence of the genome
Open accessAbstract
Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced deletions into the 5' noncoding sequence (742 nucleotides long) of the genomes of the Mahoney and Sabin 1 strains of poliovirus type 1 by using infectious cDNA clones of the virus strains. Plaque sizes shown by deletion mutants were used as a marker for rate of viral proliferation. Deletion mutants of both the strains thus constructed lacked a genome region of nucleotide positions 564 to 726. The sizes of plaques displayed by these deletion mutants were smaller than those by the respective parental viruses, although a phenotype referring to reproductive capacity at different temperatures (rct) of viruses was not affected by introduction of the deletion. Monkey neurovirulence tests were performed on the deletion mutants. The results clearly indicated that the deletion mutants had much less neurovirulence than with the corresponding parent viruses. Production of infectious particles and virus-specific protein synthesis in cells infected with the deletion mutants started later than in those infected with the parental viruses. The rate at which cytopathic effect progressed was also slower in cells infected with the mutants. Phenotypic stability of the deletion mutant for small-plaque phenotype and temperature sensitivity was investigated after passaging the mutant at an elevated temperature of 37.5 degrees C. Our data strongly suggested that the less neurovirulent phenotype introduced by the deletion is very stable during passaging of the virus.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The text we found for this paper looks like another document, so the self-check wasn’t run.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,311
Pelletier, J., & Sonenberg, N. (1988) · Nature · doi:10.1038/334320a0 · Cited by 1,953
Rapid Evolution of RNA Genomes
Holland, J. J., Spindler, K. R., Horodyski, F. M., Grabau, E. A., Nichol, S. T., & VandePol, S. (1982) · Science · doi:10.1126/science.7041255 · Cited by 1,478
Primary structure, gene organization and polypeptide expression of poliovirus RNA
Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., et al. (1981) · Nature · doi:10.1038/291547a0 · Cited by 914
Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Racaniello, V. R., & Baltimore, D. (1981) · Science · doi:10.1126/science.6272391 · Cited by 696
Cloning and expression of the gene for bacteriophage T7 RNA polymerase.
Davanloo, P., Rosenberg, A. H., Dunn, J. J., & Studier, F. W. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.7.2035 · Cited by 691
Evans, D. M. A., Dunn, G., Minor, P. D., Schild, G. C., Cann, A. J., Stanway, G., Almond, J. W., Currey, K., & Maizel, J. V. (1985) · Nature · doi:10.1038/314548a0 · Cited by 440
Complete nucleotide sequences of all three poliovirus serotype genomes
Toyoda, H., Kohara, M., Kataoka, Y., Suganuma, T., Omata, T., Imura, N., & Nomoto, A. (1984) · Journal of Molecular Biology · doi:10.1016/0022-2836(84)90084-6 · Cited by 415
Self-citationSynthesis of infectious poliovirus RNA by purified T7 RNA polymerase.
van der Werf, S., Bradley, J., Wimmer, E., Studier, F. W., & Dunn, J. J. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.8.2330 · Cited by 337
Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
Nomoto, A., Omata, T., Toyoda, H., Kuge, S., Horie, H., Kataoka, Y., Genba, Y., Nakano, Y., & Imura, N. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.19.5793 · Cited by 308
Self-citationVariation among human 28S ribosomal RNA genes.
Gonzalez, I. L., Gorski, J. L., Campen, T. J., Dorney, D. J., Erickson, J. M., Sylvester, J. E., & Schmickel, R. D. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.22.7666 · Cited by 288
Pilipenko, E. V., Blinov, V. M., Romanova, L. I., Sinyakov, A. N., Maslova, S. V., & Agol, V. I. (1989) · Virology · doi:10.1016/0042-6822(89)90259-6 · Cited by 259
The complete nucleotide sequence of a common cold virus: human rhinovlrus 14
Stanway, G., Hughes, P. J., Mountford, R., Minor, P. D., & Almond, J. W. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.20.7859 · Cited by 254
Kawamura, N., Kohara, M., Abe, S., Komatsu, T., Tago, K., Arita, M., & Nomoto, A. (1989) · Journal of Virology · doi:10.1128/jvi.63.3.1302-1309.1989 · Cited by 232
Self-citationJenkins, O. F., Booth, J. D., Minor, P. D., & Almond, J. W. (1987) · Journal of General Virology · doi:10.1099/0022-1317-68-7-1835 · Cited by 202
Lindberg, A., Stålhandske, P., & Pettersson, U. (1987) · Virology · doi:10.1016/0042-6822(87)90435-1 · Cited by 198
Genetic analysis of the attenuation phenotype of poliovirus type 1
Omata, T., Kohara, M., Kuge, S., Komatsu, T., Abe, S., Semler, B. L., Kameda, A., Itoh, H., Arita, M., & Wimmer, E. (1986) · Journal of Virology · doi:10.1128/jvi.58.2.348-358.1986 · Cited by 190
Self-citationSkern, T., Sommergruber, W., Blaas, D., Gruendler, P., Fraundorfer, F., Pieler, C., Maurer-Fogy, I., & Kuechler, E. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.6.2111 · Cited by 183
Trono, D., Andino, R., & Baltimore, D. (1988) · Journal of Virology · doi:10.1128/jvi.62.7.2291-2299.1988 · Cited by 171
Complete nucleotide sequence of the genome of coxsackievirus B1
Iizuka, N., Shusuke, K., & Nomoto, A. (1987) · Virology · doi:10.1016/0042-6822(87)90436-3 · Cited by 159
Self-citationSvitkin, Y. V., Maslova, S. V., & Agol, V. I. (1985) · Virology · doi:10.1016/0042-6822(85)90127-8 · Cited by 148
Kuge, S., & Nomoto, A. (1987) · Journal of Virology · doi:10.1128/jvi.61.5.1478-1487.1987 · Cited by 146
Self-citationMolecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14.
Callahan, P. L., Mizutani, S., & Colonno, R. J. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.3.732 · Cited by 137
Svitkin, Y. V., Pestova, T. V., Maslova, S. V., & Agol, V. I. (1988) · Virology · doi:10.1016/0042-6822(88)90510-7 · Cited by 126
Semler, B. L., Dorner, A. J., & Wimmer, E. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.12.5123 · Cited by 123
Racaniello, V. R., & Meriam, C. (1986) · Virology · doi:10.1016/0042-6822(86)90211-4 · Cited by 89
A mouse model for poliovirus neurovirulence identifies mutations that attenuate the virus for humans
Monica, N. L., Almond, J. W., & Racaniello, V. R. (1987) · Journal of Virology · doi:10.1128/jvi.61.9.2917-2920.1987 · Cited by 83
An internal 5'-noncoding region required for translation of poliovirus RNA in vitro
Bieńkowska-Szewczyk, K., & Ehrenfeld, E. (1988) · Journal of Virology · doi:10.1128/jvi.62.8.3068-3072.1988 · Cited by 80
Dildine, S. L., & Semler, B. L. (1989) · Journal of Virology · doi:10.1128/jvi.63.2.847-862.1989 · Cited by 68
Kohara, M., Omata, T., Kameda, A., Semler, B. L., Itoh, H., Wimmer, E., & Nomoto, A. (1985) · Journal of Virology · doi:10.1128/jvi.53.3.786-792.1985 · Cited by 65
Self-citationOmata, T., Kohara, M., Yuko, S., Atsuko, K., Imura, N., & Nomoto, A. (1984) · Gene · doi:10.1016/0378-1119(84)90026-x · Cited by 62
Self-citationKohara, M., Abe, S., Komatsu, T., Tago, K., Arita, M., & Nomoto, A. (1988) · Journal of Virology · doi:10.1128/jvi.62.8.2828-2835.1988 · Cited by 57
Self-citationGenetic variation occurring on the genome of an in vitro insertion mutant of poliovirus type 1
Kuge, S., Kawamura, N., & Nomoto, A. (1989) · Journal of Virology · doi:10.1128/jvi.63.3.1069-1075.1989 · Cited by 56
Self-citationKohara, M., Abe, S., Kuge, S., Semler, B. L., Komatsu, T., Arita, M., Itoh, H., & Nomoto, A. (1986) · Virology · doi:10.1016/0042-6822(86)90100-5 · Cited by 42
Self-citationStrategy for construction of live picornavirus vaccines
Nomoto, A., Iizuka, N., Kohara, M., & Arita, M. (1988) · Vaccine · doi:10.1016/s0264-410x(88)80015-x · Cited by 24
Self-citationEVALUATION OF SABIN LIVE POLIOVIRUS VACCINE IN JAPAN
Egashira, Y., Uchida, N., & Shimojo, H. (1967) · Japanese Journal of Medical Science and Biology · doi:10.7883/yoken1952.20.281 · Cited by 10
Cited by
Sorted by citations
Internal ribosome entry site within hepatitis C virus RNA
Tsukiyama–Kohara, K., Iizuka, N., Kohara, M., & Nomoto, A. (1992) · Journal of Virology · doi:10.1128/jvi.66.3.1476-1483.1992 · Cited by 925
Self-citationVACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION
Kew, O. M., Sutter, R. W., de Gourville, E. M., Dowdle, W. R., & Pallansch, M. A. (2005) · Annual Review of Microbiology · doi:10.1146/annurev.micro.58.030603.123625 · Cited by 703
Dengue Virus Structural Differences That Correlate with Pathogenesis
Leitmeyer, K. C., Vaughn, D. W., Watts, D. M., Salas, R., Villalobos, I., de Chacon, Ramos, C., & Rico-Hesse, R. (1999) · Journal of Virology · doi:10.1128/jvi.73.6.4738-4747.1999 · Cited by 622
The novel mechanism of initiation of picornavirus RNA translation
Jackson, R. J., Howell, M. T., & Kaminski, A. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90302-r · Cited by 361
Pilipenko, E. V., Gmyl, A. P., Maslova, S. V., Svitkin, Y. V., Sinyakov, A. N., & Agol, V. I. (1992) · Cell · doi:10.1016/0092-8674(92)90211-t · Cited by 276
Metadata
- Authors
- Iizuka, N., Kohara, M., Hagino-Yamagishi, K., Abe, S., Komatsu, T., Tago, K., Arita, M., & Nomoto, A.
- Affiliations
- Tokyo Metropolitan Institute of Medical Science (JP)
- Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1989-12
- Volume
- 63
- Issue
- 12
- Pages
- 5354-5363
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC251202
- OpenAlex
- W1532375795
- Cited by
- 107
- References
- 39
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 6 · 2013: 2
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Immunology Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Keywords
- poliovirus type 1, viral attenuation, neurovirulence, genomic deletions, deletion mutants, infectious clone, plaque size, temperature-sensitive mutants, cytopathic effect, phenotypic stability, Mahoney strain
- MeSH terms
- Animals, Base Sequence, Cell Line, Chromosome Deletion, DNA, Viral, Genes, Viral, HeLa Cells, Humans, Molecular Sequence Data, Mutation, Viral Plaque Assay, Plasmids, Promoter Regions, Genetic, RNA, Ribosomal, 28S, Transcription, Genetic, Transfection, Viral Proteins, Virulence, Restriction Mapping, Poliovirus
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
