Cited by 232Viral Infections and Immunology ResearchVirology and Viral DiseasesAnimal Virus Infections Studies

Determinants in the 5' noncoding region of poliovirus Sabin 1 RNA that influence the attenuation phenotype

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Kawamura, N, Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, Nomoto, A·1989·Journal of Virology

Abstract

A number of recombinants between the virulent Mahoney and attenuated Sabin strains of type 1 poliovirus were constructed by using infectious cDNA clones of the two strains. To identify a strong neurovirulence determinant(s) residing in the genome region upstream of nucleotide position 1122, these recombinant viruses were subjected to biological tests, including monkey neurovirulence tests. The results of the monkey neurovirulence tests suggested the important contribution of an adenine residue (Mahoney type) at position 480 to the expression of the neurovirulence phenotype of type 1 poliovirus. This nucleotide, however, had only a minor effect, if any, on viral temperature sensitivity. Monkey neurovirulence tests on the recombinant virus whose genome had a guanine residue (Sabin type) at position 480 and variants generated from this recombinant virus in the central nervous system of monkeys strongly suggested that only one nucleotide change, from adenine to guanine, was not sufficient for full expression of the attenuation phenotype encoded by this genome region. These results suggest that the expression of the attenuation phenotype depends on the highly ordered structure formed in the 5' noncoding sequence and that the formation of such a structure is possibly influenced by the nucleotide at position 480. Furthermore, in vitro biological tests performed on viruses recovered from the central nervous system of monkeys injected with a temperature-sensitive recombinant virus showing the small-plaque and d phenotypes revealed that most of the recovered viruses had even higher temperature sensitivities and that all of the recovered viruses that had acquired the large-plaque phenotype had lost the d phenotype to some extent. These results indicate that there may be an unknown selection pressure(s) in the central nervous system and that common determinants might be involved in the expression of the small-plaque and d phenotypes.

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Crossref · 10.1128/jvi.63.3.1302-1309.1989

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Authors
Kawamura, N., Kohara, M., Abe, S., Komatsu, T., Tago, K., Arita, M., & Nomoto, A.
Affiliations
Tokyo Metropolitan Institute of Medical Science (JP)
Venue
Journal of Virology
Published
1989-03
Volume
63
Issue
3
Pages
1302-1309
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
OpenAlex
W1641036754
Cited by
232
References
22
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 6 · 2015: 3 · 2014: 7 · 2013: 3 · 2012: 6
FWCI
14.32
Type
journal article
Language
en
Open access
Locations
  • Journal of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
Keywords
poliovirus type 1, 5' untranslated region, Mahoney strain, recombinant virus, RNA secondary structure, temperature sensitivity, infectious clone
MeSH terms
Animals, Haplorhini, Cloning, Molecular, Genes, Viral, Genetic Engineering, Nervous System, Poliovirus Vaccine, Oral, RNA, Viral, Temperature, Vaccines, Attenuated, Virus Replication, Poliovirus

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