The DOI matches its Crossref record; 38 of the 39 references it cites were found in a registry or an index.
Construction of less neurovirulent polioviruses by introducing deletions into the 5' noncoding sequence of the genome
Open accessAbstract
Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced deletions into the 5' noncoding sequence (742 nucleotides long) of the genomes of the Mahoney and Sabin 1 strains of poliovirus type 1 by using infectious cDNA clones of the virus strains. Plaque sizes shown by deletion mutants were used as a marker for rate of viral proliferation. Deletion mutants of both the strains thus constructed lacked a genome region of nucleotide positions 564 to 726. The sizes of plaques displayed by these deletion mutants were smaller than those by the respective parental viruses, although a phenotype referring to reproductive capacity at different temperatures (rct) of viruses was not affected by introduction of the deletion. Monkey neurovirulence tests were performed on the deletion mutants. The results clearly indicated that the deletion mutants had much less neurovirulence than with the corresponding parent viruses. Production of infectious particles and virus-specific protein synthesis in cells infected with the deletion mutants started later than in those infected with the parental viruses. The rate at which cytopathic effect progressed was also slower in cells infected with the mutants. Phenotypic stability of the deletion mutant for small-plaque phenotype and temperature sensitivity was investigated after passaging the mutant at an elevated temperature of 37.5 degrees C. Our data strongly suggested that the less neurovirulent phenotype introduced by the deletion is very stable during passaging of the virus.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,311
Pelletier, J., & Sonenberg, N. (1988) · Nature · doi:10.1038/334320a0 · Cited by 1,953
Rapid Evolution of RNA Genomes
Holland, J. J., Spindler, K. R., Horodyski, F. M., Grabau, E. A., Nichol, S. T., & VandePol, S. (1982) · Science · doi:10.1126/science.7041255 · Cited by 1,478
Primary structure, gene organization and polypeptide expression of poliovirus RNA
Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., et al. (1981) · Nature · doi:10.1038/291547a0 · Cited by 914
Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Racaniello, V. R., & Baltimore, D. (1981) · Science · doi:10.1126/science.6272391 · Cited by 696
Cloning and expression of the gene for bacteriophage T7 RNA polymerase.
Davanloo, P., Rosenberg, A. H., Dunn, J. J., & Studier, F. W. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.7.2035 · Cited by 691
Evans, D. M. A., Dunn, G., Minor, P. D., Schild, G. C., Cann, A. J., Stanway, G., Almond, J. W., Currey, K., & Maizel, J. V. (1985) · Nature · doi:10.1038/314548a0 · Cited by 440
Complete nucleotide sequences of all three poliovirus serotype genomes
Toyoda, H., Kohara, M., Kataoka, Y., Suganuma, T., Omata, T., Imura, N., & Nomoto, A. (1984) · Journal of Molecular Biology · doi:10.1016/0022-2836(84)90084-6 · Cited by 415
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Tsukiyama–Kohara, K., Iizuka, N., Kohara, M., & Nomoto, A. (1992) · Journal of Virology · doi:10.1128/jvi.66.3.1476-1483.1992 · Cited by 925
Self-citationVACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION
Kew, O. M., Sutter, R. W., de Gourville, E. M., Dowdle, W. R., & Pallansch, M. A. (2005) · Annual Review of Microbiology · doi:10.1146/annurev.micro.58.030603.123625 · Cited by 703
Dengue Virus Structural Differences That Correlate with Pathogenesis
Leitmeyer, K. C., Vaughn, D. W., Watts, D. M., Salas, R., Villalobos, I., de Chacon, Ramos, C., & Rico-Hesse, R. (1999) · Journal of Virology · doi:10.1128/jvi.73.6.4738-4747.1999 · Cited by 622
The novel mechanism of initiation of picornavirus RNA translation
Jackson, R. J., Howell, M. T., & Kaminski, A. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90302-r · Cited by 361
Pilipenko, E. V., Gmyl, A. P., Maslova, S. V., Svitkin, Y. V., Sinyakov, A. N., & Agol, V. I. (1992) · Cell · doi:10.1016/0092-8674(92)90211-t · Cited by 276
Metadata
- Authors
- Iizuka, N., Kohara, M., Hagino-Yamagishi, K., Abe, S., Komatsu, T., Tago, K., Arita, M., & Nomoto, A.
- Affiliations
- Tokyo Metropolitan Institute of Medical Science (JP)
- Department of Microbiology, Tokyo Metropolitan Institute of Medical Science, Japan.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1989-12
- Volume
- 63
- Issue
- 12
- Pages
- 5354-5363
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC251202
- OpenAlex
- W1532375795
- Cited by
- 107
- References
- 39
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 6 · 2013: 2
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Immunology Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Keywords
- poliovirus type 1, viral attenuation, neurovirulence, genomic deletions, deletion mutants, infectious clone, plaque size, temperature-sensitive mutants, cytopathic effect, phenotypic stability, Mahoney strain
- MeSH terms
- Animals, Base Sequence, Cell Line, Chromosome Deletion, DNA, Viral, Genes, Viral, HeLa Cells, Humans, Molecular Sequence Data, Mutation, Viral Plaque Assay, Plasmids, Promoter Regions, Genetic, RNA, Ribosomal, 28S, Transcription, Genetic, Transfection, Viral Proteins, Virulence, Restriction Mapping, Poliovirus
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