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Cited by 179Animal Virus Infections StudiesMonoclonal and Polyclonal Antibodies ResearchVirology and Viral Diseases

Monoclonal antibodies to the spike protein of feline infectious peritonitis virus mediate antibody-dependent enhancement of infection of feline macrophages

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Olsen, C W, Corapi, W V, Ngichabe, C K, Baines, J D, Scott, F W·1992·Journal of Virology
DOI 10.1128/jvi.66.2.956-965.1992PMC PMC240797
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Abstract

Antibody-dependent enhancement of virus infection is a process whereby virus-antibody complexes initiate infection of cells via Fc receptor-mediated endocytosis. We sought to investigate antibody-dependent enhancement of feline infectious peritonitis virus infection of primary feline peritoneal macrophages in vitro. Enhancement of infection was assessed, after indirect immunofluorescent-antibody labelling of infected cells, by determining the ratio between the number of cells infected in the presence and absence of virus-specific antibody. Infection enhancement was initially demonstrated by using heat-inactivated, virus-specific feline antiserum. Functional compatibility between murine immunoglobulin molecules and feline Fc receptors was demonstrated by using murine anti-sheep erythrocyte serum and an antibody-coated sheep erythrocyte phagocytosis assay. Thirty-seven murine monoclonal antibodies specific for the nucleocapsid, membrane, or spike proteins of feline infectious peritonitis virus or transmissible gastroenteritis virus were assayed for their ability to enhance the infectivity of feline infectious peritonitis virus. Infection enhancement was mediated by a subset of spike protein-specific monoclonal antibodies. A distinct correlation was seen between the ability of a monoclonal antibody to cause virus neutralization in a routine cell culture neutralization assay and its ability to mediate infection enhancement of macrophages. Infection enhancement was shown to be Fc receptor mediated by blockade of antibody-Fc receptor interaction using staphylococcal protein A. Our results are consistent with the hypothesis that antibody-dependent enhancement of feline infectious peritonitis virus infectivity is mediated by antibody directed against specific sites on the spike protein.

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Crossref · 10.1128/jvi.66.2.956-965.1992

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  • Compared with full-length S protein, RBD antigen may induce fewer non-neutralizing antibodies, lowering the risk of potential ADE of SARS-CoV-2 infection.

    “Compared with the full-length S protein, RBD antigen may induce fewer non-neutralizing antibodies, lowering the risk of potential ADE of SARS-CoV-2 infection”

    From A thermostable mRNA vaccine against COVID-19 (2020) · cites this paper as Olsen et al., 1992

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Authors
Olsen, C. W., Corapi, W. V., Ngichabe, C. K., Baines, J. D., & Scott, F. W.
Affiliations
Cornell University (US)
ORCID
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
1992-02
Volume
66
Issue
2
Pages
956-965
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.66.2.956-965.1992
PMC
PMC240797
OpenAlex
W2159989855
Cited by
179
References
42
Citations by year
2026: 3 · 2025: 2 · 2024: 6 · 2023: 3 · 2022: 8 · 2021: 10 · 2020: 25 · 2019: 5 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 5 · 2013: 2 · 2012: 2
FWCI
13.45
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
feline infectious peritonitis virus, antibody-dependent enhancement, spike protein, monoclonal antibodies, Fc receptors, virus neutralization, transmissible gastroenteritis virus, endocytosis
MeSH terms
Animals, Antibodies, Monoclonal, Ascites, Cats, Cell Line, Cells, Cultured, Immunoglobulin Fc Fragments, Macrophages, Phagocytosis, Staphylococcal Protein A, Viral Envelope Proteins, Virulence, Coronavirus, Feline, Feline Infectious Peritonitis

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