The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid protein
Open accessAbstract
The human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein contains two copies of a sequence motif, the cysteine-histidine box, that is conserved among retroviruses. To identify the functionally relevant positions of a cysteine-histidine box, each amino acid in the proximal copy of the motif was individually substituted by site-directed mutagenesis. Mutations at 5 of 14 positions abolished virus replication and reduced the viral RNA content of mutant particles to between 10 and 20% of parental levels. Mutations at other positions had either no or only a minor effect on virus replication and virion RNA content. In vitro binding of RNA to bacterially expressed mutant Pr55gag polyprotein correlated well with the effects of the mutations on particle-associated viral RNA levels. The two different copies of the motif in the HIV-1 nucleocapsid protein are not functionally equivalent, since the conversion of the proximal motif to an exact copy of the distal motif results in a defect in virus replication and a reduction in the viral RNA content of mutant particles. The simultaneous substitution of functionally relevant positions in both motifs led to a significant decline in gag protein export, indicating that the nucleocapsid domain of the gag precursor is also required for efficient assembly or release of the virion.
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Cites
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Cited by
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Metadata
- Authors
- Dorfman, T., Luban, J., Goff, S. P., Haseltine, W. A., & Göttlinger, H. G.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1993-10
- Volume
- 67
- Issue
- 10
- Pages
- 6159-6169
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1557341781
- Cited by
- 279
- References
- 44
- FWCI
- 5.04
- Type
- journal article
- Language
- en
- Open access
- green
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- Dorfman, T., Luban, J., Goff, S. P., Haseltine, W. A., & Göttlinger, H. G. (1993). Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid protein. Journal of Virology. https://doi.org/10.1128/jvi.67.10.6159-6169.1993
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