Cited by 585interferon and immune responsesImmune Response and InflammationVirology and Viral Diseases

RIG-I detects viral genomic RNA during negative-strand RNA virus infection

Open access
Rehwinkel, Jan, Tan, Choon Ping, Goubau, Delphine, Schulz, Oliver, Pichlmair, Andreas, Bier, Katja, Robb, Nicole, Vreede, Frank +3 more·2010·Cell

Abstract

RIG-I is a key mediator of antiviral immunity, able to couple detection of infection by RNA viruses to the induction of interferons. Natural RIG-I stimulatory RNAs have variously been proposed to correspond to virus genomes, virus replication intermediates, viral transcripts, or self-RNA cleaved by RNase L. However, the relative contribution of each of these RNA species to RIG-I activation and interferon induction in virus-infected cells is not known. Here, we use three approaches to identify physiological RIG-I agonists in cells infected with influenza A virus or Sendai virus. We show that RIG-I agonists are exclusively generated by the process of virus replication and correspond to full-length virus genomes. Therefore, nongenomic viral transcripts, short replication intermediates, and cleaved self-RNA do not contribute substantially to interferon induction in cells infected with these negative strand RNA viruses. Rather, single-stranded RNA viral genomes bearing 5'-triphosphates constitute the natural RIG-I agonists that trigger cell-intrinsic innate immune responses during infection.

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Crossref · 10.1016/j.cell.2010.01.020

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Authors
Rehwinkel, J., Tan, C. P., Goubau, D., Schulz, O., Pichlmair, A., Bier, K., Robb, N., Vreede, F., Barclay, W., Fodor, E., & Reis e Sousa, C.
Affiliations
  • Cancer Research UK (GB)
  • University of Oxford (GB)
Venue
Cell
Publisher
Elsevier BV
Published
2010-02
Volume
140
Issue
3
Pages
397-408
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2014960200
Cited by
585
References
88
Citations by year
2026: 17 · 2025: 33 · 2024: 30 · 2023: 27 · 2022: 23 · 2021: 43 · 2020: 31 · 2019: 30 · 2018: 33 · 2017: 20 · 2016: 36 · 2015: 32 · 2014: 44 · 2013: 54 · 2012: 46
FWCI
25.52
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Cancer Research UK
  • Fondation Bettencourt Schueller
  • Medical Research Council
  • Biotechnology and Biological Sciences Research Council
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
RIG-I, negative-strand RNA viruses, viral RNA genome, interferon induction, antiviral innate immunity, influenza A virus, Sendai virus, 5'-triphosphate RNA, RNA virus replication, RIG-I agonist
MeSH terms
DEAD Box Protein 58, Roundabout Proteins, Animals, Cell Line, Dogs, Humans, Interferons, Membrane Proteins, Nerve Tissue Proteins, Receptors, Cell Surface, Receptors, Immunologic, RNA Virus Infections, RNA Viruses, RNA, Viral, Virus Replication, Mice, DEAD-box RNA Helicases

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