The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Construction and Characterization of a Fluorescently Labeled Infectious Human Immunodeficiency Virus Type 1 Derivative
Open accessAbstract
The introduction of a label which can be detected in living cells opens new possibilities for the direct analysis of dynamic processes in virus replication, such as the transport and assembly of structural proteins. Our aim was to generate a tool for the analysis of the trafficking of the main structural protein of human immunodeficiency virus type 1 (HIV-1), Gag, as well as for the analysis of virus-host cell interactions in an authentic setting. We describe here the construction and characterization of infectious HIV derivatives carrying a label within the Gag polyprotein. Based on our initial finding that a short epitope tag could be inserted near the C terminus of the matrix domain of Gag without affecting viral replication, we constructed HIV derivatives carrying the egfp gene at the analogous position, resulting in the expression of a Gag-EGFP fusion protein in the authentic viral context. Particles displaying normal viral protein compositions were released from transfected cells, and Gag-EGFP was efficiently processed by the viral protease, yielding the expected products. Furthermore, particles with mature morphology were observed by thin-section electron microscopy. The modified virus was even found to be infectious, albeit with reduced relative infectivity. By preparing mixed particles containing equimolar amounts of Gag-EGFP and Gag, we were able to obtain highly fluorescently labeled virion preparations which displayed normal morphology and full wild-type infectivity, demonstrating that the process of HIV particle assembly displays a remarkable flexibility. The fluorescent virus derivative is a useful tool for investigating the interaction of HIV with live cells.
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Cites
Wei, X., Decker, J. M., Liu, H., Zhang, Z., Arani, R. B., Kilby, J. M., Saag, M. S., Wu, X., Shaw, G. M., & Kappes, J. C. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.6.1896-1905.2002 · Cited by 1,569
Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release
Göttlinger, H. G., Dorfman, T., Sodroski, J. G., & Haseltine, W. A. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.8.3195 · Cited by 647
Hill, C. P., Worthylake, D., Bancroft, D. P., Christensen, A. M., & Sundquist, W. I. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.7.3099 · Cited by 473
Bukrinsky, M. I., Sharova, N., McDonald, T. L., Pushkarskaya, T., Tarpley, W. G., & Stevenson, M. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.13.6125 · Cited by 451
Proteolytic refolding of the HIV‐1 capsid protein amino‐terminus facilitates viral core assembly
von Schwedler, U. K., Stemmler, T. L., Klishko, V. Y., Li, S., Albertine, K. H., Davis, D. R., & Sundquist, W. I. (1998) · The EMBO Journal · doi:10.1093/emboj/17.6.1555 · Cited by 337
Three-dimensional structure of the human immunodeficiency virus type 1 matrix protein
Massiah, M. A., Starich, M. R., Paschall, C., Summers, M. F., Christensen, A. M., & Sundquist, W. I. (1994) · Journal of Molecular Biology · doi:10.1006/jmbi.1994.1719 · Cited by 271 · cited 1×
“Consistent with this fact, the model of HIV-1 MA derived from crystallographic analyses shows that the amino terminus of MA forms a compact globular structure, whereas amino acids 105 to 121 are arranged in an extended -helix projecting away from the body of the protein (19, 27).”
Structure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein
Tang, C., Ndassa, Y., & Summers, M. F. (2002) · Nature Structural Biology · doi:10.1038/nsb806 · Cited by 205
Cited by
Sowinski, S., Jolly, C., Berninghausen, O., Purbhoo, M. A., Chauveau, A., Köhler, K., Oddos, S., Eissmann, P., Brodsky, F. M., Hopkins, C., et al. (2008) · Nature Cell Biology · doi:10.1038/ncb1682 · Cited by 754
Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly
Jouvenet, N., Neil, S. J. D., Bess, C., Johnson, M. C., Virgen, C. A., Simon, S. M., & Bieniasz, P. D. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040435 · Cited by 357
HIV-1 uncoats in the nucleus near sites of integration
Burdick, R. C., Li, C., Munshi, M., Rawson, J. M. O., Nagashima, K., Hu, W.-S., & Pathak, V. K. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1920631117 · Cited by 313
“To directly label HIV-1 CA in viral complexes, we inserted GFP between matrix and CA as previously described (27, 28).”
MethodRudnicka, D., Feldmann, J., Porrot, F., Wietgrefe, S., Guadagnini, S., Prévost, M.-C., Estaquier, J., Haase, A. T., Sol-Foulon, N., & Schwartz, O. (2009) · Journal of Virology · doi:10.1128/jvi.00282-09 · Cited by 229
Proteome analysis of the HIV-1 Gag interactome
Engeland, C. E., Brown, N. P., Börner, K., Schümann, M., Krause, E., Kaderali, L., Müller, G. A., & Kräusslich, H.-G. (2014) · Virology · doi:10.1016/j.virol.2014.04.038 · Cited by 55
Metadata
- Authors
- Müller, B., Daecke, J., Fackler, O. T., Dittmar, M. T., Zentgraf, H., & Kräusslich, H.-G.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2004-10
- Volume
- 78
- Issue
- 19
- Pages
- 10803-10813
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2118990896
- Cited by
- 215
- References
- 7
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- green
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- Müller, B., Daecke, J., Fackler, O. T., Dittmar, M. T., Zentgraf, H., & Kräusslich, H.-G. (2004). Construction and Characterization of a Fluorescently Labeled Infectious Human Immunodeficiency Virus Type 1 Derivative. Journal of Virology. https://doi.org/10.1128/jvi.78.19.10803-10813.2004
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