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PAN3 Encodes a Subunit of the Pab1p-Dependent Poly(A) Nuclease in Saccharomyces cerevisiae
Open accessAbstract
The Pab1p-dependent poly(A) nuclease (PAN) from Saccharomyces cerevisiae copurifies with polypeptides of approximately 127 and 76 kDa. Previously, it was demonstrated that the 127-kDa Pan2 protein is required for PAN activity (R. Boeck, S. Tarun, M. Reiger, J. Deardorff, S. Müller-Auer, and A.B. Sachs, J. Biol. Chem. 271:432-438, 1996). Here we demonstrate that the 76-kDa protein, encoded by the nonessential PAN3 gene, is also required for enzymatic activity. Deletion of PAN3 resulted in the loss of PAN activity in yeast extracts, and immunodepletion of Pan3p from purified PAN fractions abolished enzymatic activity. We show by coimmunoprecipitation and directed two-hybrid studies that the Pan2 and Pan3 proteins physically interact. In addition, we demonstrate that a deletion of PAN2, PAN3, or both resulted in similar increases in mRNA poly(A) tail lengths in vivo. These data strongly suggest that both Pan2p and Pan3p are required subunits of the PAN enzyme and that PAN functions in vivo to shorten mRNA poly(A) tails.
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Citations
Cites
Messenger RNA degradation in eukaryotes
Sachs, A. B. (1993) · Cell · doi:10.1016/0092-8674(93)80043-e · Cited by 846
Self-citation
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Garneau, N. L., Wilusz, J., & Wilusz, C. J. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2104 · Cited by 1,294
Brennan, C. M., & Steitz*, J. A. (2001) · Cellular and Molecular Life Sciences · doi:10.1007/pl00000854 · Cited by 1,095
Mitchell, P., Petfalski, E., Shevchenko, A., Mann, M., & Tollervey, D. (1997) · Cell · doi:10.1016/s0092-8674(00)80432-8 · Cited by 954
Tucker, M., Valencia-Sanchez, M. A., Staples, R. R., Chen, J., Denis, C. L., & Parker, R. (2001) · Cell · doi:10.1016/s0092-8674(01)00225-2 · Cited by 592
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Yamashita, A., Chang, T.-C., Yamashita, Y., Zhu, W., Zhong, Z., Chen, C.-Y. A., & Shyu, A.-B. (2005) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1016 · Cited by 472
Metadata
- Authors
- Brown, C. E., Tarun, S. Z., Boeck, R., & Sachs, A. B.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1996-10-01
- Volume
- 16
- Issue
- 10
- Pages
- 5744-5753
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2110487890
- Cited by
- 168
- References
- 1
- FWCI
- 2.97
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: PAN3 Encodes a Subunit of the Pab1p-Dependent Poly(A) Nuclease in Saccharomyces cerevisiae. https://app.cochat.ai/papers/10.1128/mcb.16.10.5744
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- Brown, C. E., Tarun, S. Z., Boeck, R., & Sachs, A. B. (1996). PAN3 Encodes a Subunit of the Pab1p-Dependent Poly(A) Nuclease in Saccharomyces cerevisiae. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.16.10.5744
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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