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Cited by 191Fungal and yeast genetics researchIon channel regulation and functionIon Channels and Receptors

The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.

A Homolog of Mammalian, Voltage-Gated Calcium Channels Mediates Yeast Pheromone-Stimulated Ca 2+ Uptake and Exacerbates the cdc1 (Ts) Growth Defect

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Paidhungat, Madan, Garrett, Stephen·1997·Molecular and Cellular Biology
DOI 10.1128/mcb.17.11.6339PMC PMC232485
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Abstract

Previous studies attributed the yeast (Saccharomyces cerevisiae) cdc1(Ts) growth defect to loss of an Mn2+-dependent function. In this report we show that cdc1(Ts) temperature-sensitive growth is also associated with an increase in cytosolic Ca2+. We identified two recessive suppressors of the cdc1(Ts) temperature-sensitive growth which block Ca2+ uptake and accumulation, suggesting that cytosolic Ca2+ exacerbates or is responsible for the cdc1(Ts) growth defect. One of the cdc1(Ts) suppressors is identical to a gene, MID1, recently implicated in mating pheromone-stimulated Ca2+ uptake. The gene (CCH1) corresponding to the second suppressor encodes a protein that bears significant sequence similarity to the pore-forming subunit (alpha1) of plasma membrane, voltage-gated Ca2+ channels from higher eukaryotes. Strains lacking Mid1 or Cch1 protein exhibit a defect in pheromone-induced Ca2+ uptake and consequently lose viability upon mating arrest. The mid1delta and cch1delta mutants also display reduced tolerance to monovalent cations such as Li+, suggesting a role for Ca2+ uptake in the calcineurin-dependent ion stress response. Finally, mid1delta cch1delta double mutants are, by both physiological and genetic criteria, identical to single mutants. These and other results suggest Mid1 and Cch1 are components of a yeast Ca2+ channel that may mediate Ca2+ uptake in response to mating pheromone, salt stress, and Mn2+ depletion.

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  1. 10.1128/mcb.14.12.8259-8271.1994 · DOI
  2. Sprague, G. F., and J. W. Thorner. 1992. Pheromone response and signaltransduction during the mating process of Saccharomyces cerevisiae, p. 657–744. In E. W. Jones, J. R. Pringle, and J. R. Broach (ed.), The molecular and cellular biology of the yeast Saccharomyces. Gene expression. Cold Spring Harbor Laboratory Press, Plainview, N.Y.

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Metadata

Authors
Paidhungat, M., & Garrett, S.
Affiliations
Duke University (US)
ORCID
Garrett, Stephen
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
1997-11
Volume
17
Issue
11
Pages
6339-6347
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.17.11.6339
PMC
PMC232485
OpenAlex
W2162829341
Cited by
191
References
40
Citations by year
2026: 1 · 2025: 4 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 11 · 2016: 9 · 2015: 6 · 2014: 8 · 2013: 8 · 2012: 12
FWCI
2.02
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
Keywords
voltage-gated calcium channels, calcium uptake, MID1, mating pheromone signaling, intracellular calcium, lithium tolerance, manganese depletion, salt stress, Saccharomyces cerevisiae
MeSH terms
Amino Acid Sequence, Biological Transport, Calcium, Fungal Proteins, Membrane Glycoproteins, Molecular Sequence Data, Mutation, Pheromones, Saccharomyces cerevisiae, Suppression, Genetic, Calcium Channels, Ion Channel Gating, Gene Expression, Sequence Homology, Amino Acid, Cell Cycle Proteins, Saccharomyces cerevisiae Proteins

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