MGA2 Is Involved in the Low-Oxygen Response Element-Dependent Hypoxic Induction of Genes in Saccharomyces cerevisiae

Jiang, Yide; Vasconcelles, Michael J.; Wretzel, Sharon; Light, Anne; Martin, Charles E.; Goldberg, Mark A.

doi:10.1128/mcb.21.18.6161-6169.2001

Cited by 95Fungal and yeast genetics researchCancer, Hypoxia, and MetabolismGenomics and Chromatin Dynamics

MGA2 Is Involved in the Low-Oxygen Response Element-Dependent Hypoxic Induction of Genes in Saccharomyces cerevisiae

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Jiang, Yide, Vasconcelles, Michael J., Wretzel, Sharon, Light, Anne, Martin, Charles E., Goldberg, Mark A.·2001·Molecular and Cellular Biology

DOI 10.1128/mcb.21.18.6161-6169.2001PMC PMC87333

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Abstract

Eukaryotes have the ability to respond to changes in oxygen tension by alterations in gene expression. For example, OLE1 expression in Saccharomyces cerevisiae is upregulated under hypoxic conditions. Previous studies have suggested that the pathway regulating OLE1 expression by unsaturated fatty acids may involve Mga2p and Spt23p, two structurally and functionally related proteins. To define the possible roles of each of these genes on hypoxia-induced OLE1 expression, we examined OLE1 expression under normoxia, hypoxia, and cobalt treatment conditions in Deltamga2 or Deltaspt23 deletion strains. The results of OLE1 promoter-lacZ reporter gene and Northern blot analyses showed that hypoxia- and cobalt-induced OLE1 expression was dramatically decreased in a Deltamga2 strain but not in a Deltaspt23 strain. Further analyses using low-oxygen response element (LORE)-CYC1-lacZ fusion reporter assays and electrophoretic mobility shift assays (EMSAs) demonstrated that MGA2 significantly affects the LORE-dependent hypoxic induction pathway of gene expression. When MGA2 was supplied by a plasmid, the LORE-dependent hypoxia-inducible reporter expression was recovered, as was the hypoxia-inducible complex in EMSAs in the S. cerevisiae Deltamga2 strain. Supershift analysis of EMSAs using crude extracts containing mycMga2p indicated that Mga2p is a component of the LORE-binding complex. Another LORE-dependent, hypoxia-inducible gene, ATF1, was similarly affected in the Deltamga2 strain. These results indicate that MGA2 is required for the LORE-dependent hypoxic gene induction in S. cerevisiae.

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Oxygen depletion also enhances the expression of the oxygen-dependent fatty acid desaturase Ole1 through the Mga2 transcriptional factor.
“Oxygen depletion also enhances the expression of the oxygen-dependent fatty acid desaturase Ole1 through the Mga2 transcriptional factor [97].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [97]

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Mga2p Processing by Hypoxia and Unsaturated Fatty Acids in Saccharomyces cerevisiae : Impact on LORE-Dependent Gene Expression
Jiang, Y., Vasconcelles, M. J., Wretzel, S., Light, A., Gilooly, L., McDaid, K., Oh, C., Martin, C. E., & Goldberg, M. A. (2002) · Eukaryotic Cell · doi:10.1128/ec.1.3.481-490.2002 · Cited by 52 · see citing works
A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis
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Identification and Characterization of a Low Oxygen Response Element Involved in the Hypoxic Induction of a Family ofSaccharomyces cerevisiae Genes
Vasconcelles, M. J., Jiang, Y., McDaid, K., Gilooly, L., Wretzel, S., Porter, D. L., Martin, C. E., & Goldberg, M. A. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m009546200 · Cited by 114 · see citing works
Transition metal induction of the S. cerevisiae OLE1 gene occurs through multiple promoter elements.
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The hypoxic transcription factor KlMga2 mediates the response to oxidative stress and influences longevity in the yeast Kluyveromyces lactis
Santomartino, R., Camponeschi, I., Polo, G., Immesi, A., Rinaldi, T., Mazzoni, C., Brambilla, L., & Bianchi, M. M. (2019) · FEMS Yeast Research · doi:10.1093/femsyr/foz020 · Cited by 11 · see citing works

Authors: Jiang, Y., Vasconcelles, M. J., Wretzel, S., Light, A., Martin, C. E., & Goldberg, M. A.
Affiliations: Brigham and Women's Hospital (US)
Rutgers, The State University of New Jersey (US)
Corresponding author: Goldberg, Mark A.
ORCID: Jiang, Yide
Martin, Charles E.
Venue: Molecular and Cellular Biology
Publisher: Informa UK Limited
Published: 2001-09-01
Volume: 21
Issue: 18
Pages: 6161-6169
ISSN: 0270-7306 (ISSN-L)
1098-5549
DOI: 10.1128/mcb.21.18.6161-6169.2001
PMC: PMC87333
OpenAlex: W2132946993
Cited by: 95
References: 37
Citations by year: 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 6 · 2019: 6 · 2018: 3 · 2017: 5 · 2016: 5 · 2015: 3 · 2014: 1 · 2013: 3 · 2012: 9
FWCI: 1.98
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Molecular and Cellular Biology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r01dk045098-08, DK45098)
National Institute of General Medical Sciences (GM45768, R01GM045768)
National Institute of Diabetes and Digestive and Kidney Diseases (DK-45098)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords: hypoxia response, OLE1, ATF1, gene expression regulation, unsaturated fatty acids, transcriptional regulation, Saccharomyces cerevisiae
MeSH terms: Fungal Proteins, Membrane Proteins, Oxygen, Saccharomyces cerevisiae, Stearoyl-CoA Desaturase, Transcription Factors, Transcriptional Activation, Trans-Activators, Cell Hypoxia, Gene Expression Regulation, Fungal, Saccharomyces cerevisiae Proteins, Fatty Acid Desaturases

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MGA2 Is Involved in the Low-Oxygen Response Element-Dependent Hypoxic Induction of Genes in Saccharomyces cerevisiae

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