The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A Mammalian Bromodomain Protein, Brd4, Interacts with Replication Factor C and Inhibits Progression to S Phase
Open accessAbstract
Brd4 belongs to the BET family of nuclear proteins that carry two bromodomains implicated in the interaction with chromatin. Expression of Brd4 correlates with cell growth and is induced during early G(1) upon mitogenic stimuli. In the present study, we investigated the role of Brd4 in cell growth regulation. We found that ectopic expression of Brd4 in NIH 3T3 and HeLa cells inhibits cell cycle progression from G(1) to S. Coimmunoprecipitation experiments showed that endogenous and transfected Brd4 interacts with replication factor C (RFC), the conserved five-subunit complex essential for DNA replication. In vitro analysis showed that Brd4 binds directly to the largest subunit, RFC-140, thereby interacting with the entire RFC. In line with the inhibitory activity seen in vivo, recombinant Brd4 inhibited RFC-dependent DNA elongation reactions in vitro. Analysis of Brd4 deletion mutants indicated that both the interaction with RFC-140 and the inhibition of entry into S phase are dependent on the second bromodomain of Brd4. Lastly, supporting the functional importance of this interaction, it was found that cotransfection with RFC-140 reduced the growth-inhibitory effect of Brd4. Taken as a whole, the present study suggests that Brd4 regulates cell cycle progression in part by interacting with RFC.
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Cites
Structure and ligand of a histone acetyltransferase bromodomain
Dhalluin, C., Carlson, J. E., Zeng, L., He, C., Aggarwal, A. K., Zhou, M.-M., & Zhou, M.-M. (1999) · Nature · doi:10.1038/20974 · Cited by 1,732
Wang, Y., Cortez, D., Yazdi, P., Neff, N., Elledge, S. J., & Qin, J. (2000) · Genes & Development · doi:10.1101/gad.14.8.927 · Cited by 1,221 · cited 1×
“Moreover, RFC has been shown to interact with other factors, including the BRCA-1 complexes (45) and C/EBP (22).”
BackgroundWAF1 retards S-phase progression primarily by inhibition of cyclin-dependent kinases
Ogryzko, V. V., Wong, P., & Howard, B. H. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.8.4877 · Cited by 193
Cited by
Brd4 Coactivates Transcriptional Activation of NF-κB via Specific Binding to Acetylated RelA
Huang, B., Yang, X.-D., Zhou, M.-M., Ozato, K., & Chen, L.-F. (2009) · Molecular and Cellular Biology · doi:10.1128/mcb.01365-08 · Cited by 579
Self-citationFrench, C. A., Ramirez, C. L., Kolmakova, J., Hickman, T. T., Cameron, M. J., Thyne, M. E., Kutok, J. L., Toretsky, J. A., Tadavarthy, A. K., Kees, U. R., et al. (2008) · Oncogene · doi:10.1038/sj.onc.1210852 · Cited by 441
Use of Epigenetic Drugs in Disease: An Overview
Heerboth, S., Lapinska, K., Snyder, N., Leary, M., Rollinson, S., & Sarkar, S. (2014) · Genetics & Epigenetics · doi:10.4137/geg.s12270 · Cited by 326
Pivot-Pajot, C., Caron, C., Govin, J., Vion, A., Rousseaux, S., & Khochbin, S. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.15.5354-5365.2003 · Cited by 285
Clinical trials for BET inhibitors run ahead of the science
Andrieu, G., Belkina, A. C., & Denis, G. V. (2016) · Drug Discovery Today: Technologies · doi:10.1016/j.ddtec.2016.06.004 · Cited by 247
Metadata
- Authors
- Maruyama, T., Farina, A., Dey, A., Cheong, J., Bermudez, V. P., Tamura, T., Sciortino, S., Shuman, J., Hurwitz, J., & Ozato, K.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2002-09-01
- Volume
- 22
- Issue
- 18
- Pages
- 6509-6520
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2171262570
- Cited by
- 165
- References
- 3
- FWCI
- 2.16
- Type
- journal article
- Language
- en
- Open access
- green
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- Maruyama, T., Farina, A., Dey, A., Cheong, J., Bermudez, V. P., Tamura, T., Sciortino, S., Shuman, J., Hurwitz, J., & Ozato, K. (2002). A Mammalian Bromodomain Protein, Brd4, Interacts with Replication Factor C and Inhibits Progression to S Phase. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.22.18.6509-6520.2002
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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