The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
RhoE Binds to ROCK I and Inhibits Downstream Signaling
Open accessAbstract
RhoE belongs to the Rho GTPase family, the members of which control actin cytoskeletal dynamics. RhoE induces stress fiber disassembly in a variety of cell types, whereas RhoA stimulates stress fiber assembly. The similarity of RhoE and RhoA sequences suggested that RhoE might compete with RhoA for interaction with its targets. Here, we show that RhoE binds ROCK I but none of the other RhoA targets tested. The interaction of RhoE with ROCK I was confirmed by coimmunoprecipitation of the endogenous proteins, and the two proteins colocalized on the trans-Golgi network in COS-7 cells. Although RhoE and RhoA were not able to bind ROCK I simultaneously, RhoE bound to the amino-terminal region of ROCK I encompassing the kinase domain, at a site distant from the carboxy-terminal RhoA-binding site. Overexpression of RhoE inhibited ROCK I-induced stress fiber formation and phosphorylation of the ROCK I target myosin light chain phosphatase. These data suggest that RhoE induces stress fiber disassembly by directly binding ROCK I and inhibiting it from phosphorylating downstream targets.
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Cites
Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing
Sebbagh, M., Renvoizé, C., Hamelin, J., Riché, N., Bertoglio, J., & Bréard, J. (2001) · Nature Cell Biology · doi:10.1038/35070019 · Cited by 915
Ras signalling on the endoplasmic reticulum and the Golgi
Chiu, V. K., Bivona, T., Hach, A., Sajous, J. B., Silletti, J., Wiener, H., Johnson, R. L., Cox, A. D., & Philips, M. R. (2002) · Nature Cell Biology · doi:10.1038/ncb783 · Cited by 594 · cited 1×
“Nevertheless, other GTPases localize to the Golgi complex, including Cdc42, TC10, and RhoG (3, 8, 20), and Ras proteins have been shown elsewhere to be active on the Golgi complex (6).”
BackgroundLi, X., Bu, X., Lu, B., Avraham, H., Flavell, R. A., & Lim, B. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.4.1158-1171.2002 · Cited by 153
Cited by
ROCKs: multifunctional kinases in cell behaviour
Riento, K., & Ridley, A. J. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1128 · Cited by 1,941
Self-citationBurridge, K., & Wennerberg, K. (2004) · Cell · doi:10.1016/s0092-8674(04)00003-0 · Cited by 1,578
Rho‐kinase/ROCK: A key regulator of the cytoskeleton and cell polarity
Amano, M., Nakayama, M., & Kaibuchi, K. (2010) · Cytoskeleton · doi:10.1002/cm.20472 · Cited by 994
Pellegrin, S., & Mellor, H. (2007) · Journal of Cell Science · doi:10.1242/jcs.018473 · Cited by 704
Rho-family GTPases: it's not only Rac and Rho (and I like it)
Wennerberg, K., & Der, C. J. (2004) · Journal of Cell Science · doi:10.1242/jcs.01118 · Cited by 613
Metadata
- Authors
- Riento, K., Guasch, R. M., Garg, R., Jin, B., & Ridley, A. J.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2003-06-01
- Volume
- 23
- Issue
- 12
- Pages
- 4219-4229
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2149194464
- Cited by
- 303
- References
- 3
- FWCI
- 6.65
- Type
- journal article
- Language
- en
- Open access
- green
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- Riento, K., Guasch, R. M., Garg, R., Jin, B., & Ridley, A. J. (2003). RhoE Binds to ROCK I and Inhibits Downstream Signaling. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.23.12.4219-4229.2003
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