Cited by 172Fungal and yeast genetics researchCancer, Hypoxia, and MetabolismGenomics and Chromatin Dynamics

Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

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Todd, Bridget L., Stewart, Emerson V., Burg, John S., Hughes, Adam L., Espenshade, Peter J.·2006·Molecular and Cellular Biology

Abstract

Fission yeast sterol regulatory element binding protein (SREBP), called Sre1p, functions in an oxygen-sensing pathway to allow adaptation to fluctuating oxygen concentrations. The Sre1p-Scp1p complex responds to oxygen-dependent sterol synthesis as an indirect measure of oxygen availability. To examine the role of Sre1p in anaerobic gene expression in Schizosaccharomyces pombe, we performed transcriptional profiling experiments after a shift to anaerobic conditions for 1.5 h. Of the 4,940 genes analyzed, expression levels of 521 (10.5%) and 686 (13.9%) genes were significantly increased and decreased, respectively, under anaerobic conditions. Sre1p controlled 68% of genes induced > or = 2-fold. Oxygen-requiring biosynthetic pathways for ergosterol, heme, sphingolipid, and ubiquinone were primary targets of Sre1p. Induction of glycolytic genes and repression of mitochondrial oxidative phosphorylation genes largely did not require Sre1p. Using chromatin immunoprecipitation, we demonstrated that Sre1p acts directly at target gene promoters and stimulates its own transcription under anaerobic conditions. sre1+ promoter analysis identified two DNA elements that are both necessary and sufficient for oxygen-dependent, Sre1p-dependent transcription. Interestingly, these elements are homologous to sterol regulatory elements bound by mammalian SREBP, highlighting the evolutionary conservation between Sre1p and SREBP. We conclude that Sre1p is a principal activator of anaerobic gene expression, upregulating genes required for nonrespiratory oxygen consumption.

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Crossref · 10.1128/mcb.26.7.2817-2831.2006

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Authors
Todd, B. L., Stewart, E. V., Burg, J. S., Hughes, A. L., & Espenshade, P. J.
Affiliations
Johns Hopkins University (US)
Venue
Molecular and Cellular Biology
Published
2006-04-01
Volume
26
Issue
7
Pages
2817-2831
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2104657811
Cited by
172
References
37
Citations by year
2026: 2 · 2025: 5 · 2024: 6 · 2023: 8 · 2022: 4 · 2021: 5 · 2020: 8 · 2019: 6 · 2018: 5 · 2017: 9 · 2016: 7 · 2015: 11 · 2014: 5 · 2013: 6 · 2012: 11
FWCI
6.26
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (R01HL077588, HL 077588)
  • National Institute of General Medical Sciences (GM007445, T32GM007445)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
SREBP, anaerobic gene expression, oxygen sensing, Schizosaccharomyces pombe, transcriptional regulation, sterol biosynthesis, ergosterol biosynthesis, heme biosynthesis, sphingolipid biosynthesis, ubiquinone biosynthesis, promoter elements
MeSH terms
Anaerobiosis, Genes, Fungal, Oxygen, Promoter Regions, Genetic, Schizosaccharomyces, Time Factors, Down-Regulation, Up-Regulation, Gene Expression Regulation, Fungal, Schizosaccharomyces pombe Proteins, Regulatory Elements, Transcriptional

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