The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.
Open accessAbstract
We have isolated a yeast conditional mutant which rapidly ceases synthesis of mRNA when subjected to the nonpermissive temperature. This mutant (rpb1-1) was constructed by replacing the wild-type chromosomal copy of the gene encoding the largest subunit of RNA polymerase II with one mutagenized in vitro. The rapid cessation of mRNA synthesis in vivo and the lack of RNA polymerase II activity in crude extracts indicate that the mutant possesses a functionally defective, rather than an assembly-defective, RNA polymerase II. The shutdown in mRNA synthesis in the rpb1-1 mutant has pleiotropic effects on the synthesis of other RNAs and on the heat shock response. This mutant provides direct evidence that the RPB1 protein has a functional role in mRNA synthesis.
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Cites
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Cited by
Dissecting the regulatory circuitry of a eukaryotic genome
Holstege, F. C. P., Jennings, E. G., Wyrick, J. J., Lee, T. I., Hengartner, C. J., Green, M. R., Golub, T. R., Lander, E. S., & Young, R. A. (1998) · Cell · doi:10.1016/s0092-8674(00)81641-4 · Cited by 1,670
Self-citationCodon Optimality Is a Major Determinant of mRNA Stability
Presnyak, V., Alhusaini, N., Chen, Y.-H., Martin, S., Morris, N., Kline, N., Olson, S., Weinberg, D., Baker, K. E., Graveley, B. R., et al. (2015) · Cell · doi:10.1016/j.cell.2015.02.029 · Cited by 1,078
“RNA-Seq rpb1-1 mutant cells (Nonet et al., 1987) were grown to mid-log phase at 24 C and shifted to a non-permissive temperature of 37 C.”
MethodInfluentialMessenger RNA degradation in eukaryotes
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Precision and functional specificity in mRNA decay
Wang, Y., Liu, C. L., Storey, J. D., Tibshirani, R. J., Herschlag, D., & Brown, P. O. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.092538799 · Cited by 707
Transcription Elongation Factors Repress Transcription Initiation from Cryptic Sites
Kaplan, C. D., Laprade, L., & Winston, F. (2003) · Science · doi:10.1126/science.1087374 · Cited by 585
Metadata
- Authors
- Nonet, M., Scafe, C., Sexton, J., & Young, R.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1987-05
- Volume
- 7
- Issue
- 5
- Pages
- 1602-1611
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1938794721
- Cited by
- 347
- References
- 24
- FWCI
- 3.70
- Type
- journal article
- Language
- en
- Open access
- green
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- Nonet, M., Scafe, C., Sexton, J., & Young, R. (1987). Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.7.5.1602
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
