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Cited by 38Genomics and Phylogenetic StudiesViral Infectious Diseases and Gene Expression in InsectsVirology and Viral Diseases

A Multicenter Study To Evaluate the Performance of High-Throughput Sequencing for Virus Detection

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Khan, Arifa S., Ng, Siemon H. S., Vandeputte, Olivier, Aljanahi, Aisha, Deyati, Avisek, Cassart, Jean-Pol, Charlebois, Robert L., Taliaferro, Lanyn P.·2017·mSphere
DOI 10.1128/msphere.00307-17PMC PMC5597969
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Abstract

Recent high-throughput sequencing (HTS) investigations have resulted in unexpected discoveries of known and novel viruses in a variety of sample types, including research materials, clinical materials, and biological products. Therefore, HTS can be a powerful tool for supplementing current methods for demonstrating the absence of adventitious or unwanted viruses in biological products, particularly when using a new cell line. However, HTS is a complex technology with different platforms, which needs standardization for evaluation of biologics. This collaborative study was undertaken to investigate detection of different virus types using two different HTS platforms. The results of the independently performed studies demonstrated a similar sensitivity of virus detection, regardless of the different sample preparation and processing procedures and bioinformatic analyses done in the three laboratories. Comparable HTS detection of different virus types supports future development of reference virus materials for standardization and validation of different HTS platforms.

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  • Developing media formulations that do not require animal- or human-sourced raw materials is used in the recombinant protein manufacturing industry.

    “A better strategy, also used in the recombinant protein manufacturing industry, is to develop media formulations that do not require animal-or human-sourced raw materials 58 .”

    From Viral contamination in biologic manufacture and implications for emerging therapies (2020) · cites this paper as [58]

    The Materials and Methods (Cells; page not provided) describe media containing fetal bovine serum, while the Discussion concerns virus-detection methods. Searches for animal- or human-source-free media formulations and their use in recombinant protein manufacturing found no passage addressing the claim. (LLM, conf=0.76)

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Cited by

Sorted by citations

Authors
Khan, A. S., Ng, S. H. S., Vandeputte, O., Aljanahi, A., Deyati, A., Cassart, J.-P., Charlebois, R. L., & Taliaferro, L. P.
Affiliations
  • Center for Biologics Evaluation and Research (US)
  • Sanofi (Canada) (CA)
  • GlaxoSmithKline (Belgium) (BE)
Corresponding author
Khan, Arifa S.
ORCID
Venue
mSphere
Publisher
American Society for Microbiology
Published
2017-10-25
Volume
2
Issue
5
ISSN
2379-5042 (ISSN-L)
DOI
10.1128/msphere.00307-17
PMC
PMC5597969
OpenAlex
W2756055962
Cited by
38
References
5
Citations by year
2026: 2 · 2025: 7 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 7 · 2018: 4
FWCI
1.23
Type
journal article
Language
en
Open access
Licence
Locations
  • mSphere (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
next-generation sequencing, virus detection, biological products, detection sensitivity, standardization, bioinformatics analysis, sample preparation

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